Another culprit is static. Moisture helps to keep the static down.
"Clough, Bret" <clo...@medicine.tamhsc.edu> Sent by: histonet-boun...@lists.utsouthwestern.edu 04/21/2011 12:11 PM To Histonet list serv. <histonet@lists.utsouthwestern.edu> cc Subject [Histonet] How to keep paraffin sections from sticking to blade/blade holder. While sectioning paraffin embedded tissue on a rotary microtome I noticed the sections sticking to the blade holder and bunching up. What do I need to do so that my section come off the blade in a ribbon? Please note that this is my first time in sectioning tissue and this is a learning exercise for me. Thanks in advance for your suggestions . Bret _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet