Dr. Dean,
Histotechs are familiar with brain degeneration "Silver Stains" on FFPE cut
sections(slides). Common silver stains include Bielchowsky's method for
neuritic plaques and neurofibrillary tangles (most requested by my pathologists
for degenerative brain disorders), Bodian's method for nerve endings and fibers
, Holmes method for axons and nerve fibers, or Palmgren's silver impregnation
method for neural fibers. There are other histochemical stains, but these are
most common on silvers on brain sections. We have been making our own
solutions for years (and years and years...).
There are some kits for staining available through vendors such as Newcomer
Supply http://www.newcomersupply.com/products-standard-special-stains.php, or
American Master Tech
http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStyles&item=KTBIE,
and if you know the individual reagents you need, you can buy them from almost
all vendors.
I have not used (nor have an affiliation with) Newcomer Supply and American
Master Tech brain kits, but their other products have been great.
As for staining whole chunks, I have not seen or done this. It sounds
interesting.
Hope this helps,
Hugh, HTL (ASCP)
Pathology core manager
Hawaii
> From: robin_d...@compbio.com
> To: histonet@lists.utsouthwestern.edu
> Date: Mon, 25 Apr 2011 15:09:49 -0700
> Subject: [Histonet] silver stain on slide-mounted FFPE brain tissues
>
> Hi Histonetters,
>
>
>
> We want to do a degeneration-selective silver stain on slide-mounted
> formalin-fixed paraffin-embedded monkey brain sections. It looks like most
> of the kits/protocols are for free floating tissue sections or chunks of
> tissue that are stained and then sectioned. Has anyone used this stain or a
> similar stain on slide -mounted brain sections? (something more like an
> IHC protocol). We need to do multiple different stains on sections from the
> same brain, so we don't want to silver stain a whole chunk of brain tissue,
> just some individual sections. I'd appreciate any suggestions or help anyone
> might have to offer.
>
>
>
> Thank you,
>
>
>
> Robin
>
>
>
> Robin R. Dean, Ph.D.
>
> Senior Scientist & Study Director
>
> Comparative Biosciences, Inc.
>
> 786 Lucerne Dr.
>
> Sunnyvale, CA
>
> (408) 738-8060
>
> robin_d...@compbio.com
>
>
>
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