I am afraid your "decision" is wrong. "Empty nuclei" appears when the water between the section and the slide is not completely removed and "dissolves" the nuclear contents while drying and no staining after wards. Check the sections draining for this artifact. René J.
--- On Wed, 4/27/11, Marshall, Kimberly K <kkmarsh...@anthc.org> wrote: From: Marshall, Kimberly K <kkmarsh...@anthc.org> Subject: [Histonet] Biopsy Run To: histonet@lists.utsouthwestern.edu Date: Wednesday, April 27, 2011, 8:21 AM Morning and Happy Hump day Histo peeps Hope everyone is having a fun filled Lab Week, Here is Alaska we are eating more than usual... Just need to ask one more question regarding my evil "nuclear artifact". We have decided it is a processing issue due to the type of cassettes we use. In cutting down the processing time, the reagents are not able to get through the mesh on the cassettes. I have ordered other kinds but due to limited funds (cant get a new labeler) and even with the special pens for writing on cassettes and slides the hand written numbers are fading, I cant use them. My next thought is to lengthen my biopsy run. Would you folks out there share your biopsy run times with me? I don't want to go back to having the over processing issues with the biopsies that occur in the 12 hour, but maybe adding time would help here. Thanks in advance and again hope everyone is having a great Lab week. Kim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet