At 100µL per dispense, to do 50 tests you will need 50 x 100µL = 5000 µL = 5 ml You do need to dilute it René J.
--- On Thu, 4/28/11, Angela Bitting <akbitt...@geisinger.edu> wrote: From: Angela Bitting <akbitt...@geisinger.edu> Subject: Re: [Histonet] Ventana Probes To: "Angela Bitting" <akbitt...@geisinger.edu>, "histonet" <histonet@lists.utsouthwestern.edu> Date: Thursday, April 28, 2011, 11:10 AM Sorry folks, in my impaired state of mind I made a boo boo. I need 5-6 ml to do 50 tests. If the dispenses are still 100 ul each. Any thoughts? >>> "Angela Bitting" <akbitt...@geisinger.edu> 4/28/2011 11:03 AM >>> Ok. Ventana is on my last nerve with this new probe configuration of theirs. I have a 4 ml vial of EBER Probe here in my lab. I am told that this is good for approx 50 tests. So at 100 ul a dispense, I need to bring the volume up to at least 15 ml (probably 16ml). Not a soul under Ventana's employ will tell me what probe diluent to use, or what else I am supposed to do with it. I'm ready to add vodka. If anyone out there has transitioned to the new EBER probe configuration, can you give a gal a break and contact me? Sorry for the Thursday rant....anyway Happy Lab Week everyone!!! Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
