ANTIBODY LOTS I have a Dako and I have a Bond too. For the Dako I use my positive control. I use 3 slides. One is treated as the negative control - no antibody One is treated with the current lot One is treated with the new lot. I keep all QC slides together. I keep the 3 slides together.
For my Bond, I use 2 slides of my positive control When a new lot comes in I run the new lot and the negative control. Then I compare them to the previous lot that I ran in the past. I keep the slides in chronological order - the previous lot goes back with The slides from that date. The NEW Lot slides get filed with today's date. DETECTION SYSTEM LOTS For the Bond I QC the detection kit using an antibody like Ki-67, it works well and always shows good nuclear staining. I indicate the received date, and QC date. I run the QC on my Ki-67 positive control. I use 2 slides. One is treated as the negative control - no antibody. One is treated with Ki-67. I compare these to the previous Detection kit QC slides I created a written procedures for all of the above. Hope this helps. Dana Settembre Immunohistochemistry Lab University Hospital - UMDNJ Newark, NJ -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Friday, April 29, 2011 12:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cap new ANP.22760 hi everyone, just wondering how others were handling this checklist statement? thanks so much for your input. seems like a lot of extra work when running control with each show if each is working. anita dudley providence hosp mobile alabama _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet