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Today's Topics:
1. Re: Diff-Quik stain (Robert Richmond)
2. chicken immunohistochemistry (mohamed abd el razik)
3. Re: MSH 2011 Symposium (David Kemler)
4. IMEB (Sheila Haas)
5. RE: IMEB (Kaye Ryan)
6. RE: IMEB (sgoe...@mirnarx.com)
7. Re: IMEB (Sheila Haas)
8. RE: IMEB (Bartlett, Jeanine (CDC/OID/NCEZID))
9. RE: IMEB (Rene J Buesa)
10. Ventana Probes (Angela Bitting)
11. Re: Ventana Probes (Angela Bitting)
12. Re: Ventana Probes (Rene J Buesa)
13. Re: Ventana Probes (Rene J Buesa)
14. TNF alpha antibody (Michele Wich)
15. RE: TNF alpha antibody (Liz Chlipala)
16. TNF-alpha (Liz Chlipala)
----------------------------------------------------------------------
Message: 1
Date: Wed, 27 Apr 2011 21:47:10 -0400
From: Robert Richmond<rsrichm...@gmail.com>
Subject: [Histonet] Re: Diff-Quik stain
To: histonet@lists.utsouthwestern.edu
Message-ID:<BANLkTinSkBO2wNjMjEFiuRsVU=s8qb_...@mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Diff-Quik is a trade name - belongs to whatever Scientific Products is
called this week. It's often used generically for all two-stain
Romanowsky type stains, since there isn't a really satisfactory
generic name for them.
I've used several generic stains with separate eosin ("xanthine") and
blue ("thiazine") dye baths, and generally found them satisfactory.
But you do need to try them out for yourself.
Bob Richmond
Samurai Pathologist
Knoxville TN
------------------------------
Message: 2
Date: Thu, 28 Apr 2011 01:10:06 -0700 (PDT)
From: mohamed abd el razik<k8...@yahoo.com>
Subject: [Histonet] chicken immunohistochemistry
To: Histonet@lists.utsouthwestern.edu
Message-ID:<496385.41591...@web112602.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
dear all
i'm looking for vendors or companies that we could order some anti chicken antibodies
(primary& secondary). speacially for CD4& CD8
i'm new in that feild and need your help.
mohamed
------------------------------
Message: 3
Date: Thu, 28 Apr 2011 03:12:07 -0700 (PDT)
From: David Kemler<histot...@yahoo.com>
Subject: Re: [Histonet] MSH 2011 Symposium
To: Fellow HistoNetters<Histonet@Lists.UTSouthwestern.edu>
Message-ID:<177783.69813...@web120605.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi Amber -
I have the MSH Symposium scheduled to begin promos on May 8th's HistoTALK.
Hopefully, the announcements will help get listeners attention and get them to
attend. I'll be sure to mention your email on the show. :- )
Yours,
Dave
www.HistoTALK.com
________________________________
From: Amber McKenzie<amber.mcken...@gastrodocs.net>
Cc: Histonet list serv.<histonet@lists.utsouthwestern.edu>
Sent: Wed, April 27, 2011 12:08:14 PM
Subject: [Histonet] MSH 2011 Symposium
If anyone is interested in attending the annual Mississippi Society for
Histotechnology Symposium June 24-26th in Hattiesburg, MS, please
contact me directly and I can email you a program! We'd love to have
you :)
Amber McKenzie, BS, HT(ASCP)
MSH Secretary
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Thu, 28 Apr 2011 04:17:14 -0700 (PDT)
From: Sheila Haas<micropathl...@yahoo.com>
Subject: [Histonet] IMEB
To: histonet@lists.utsouthwestern.edu
Message-ID:<967305.23545...@web161712.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Thanks to everyone for the feedback on IMEB. It appears there have only been a
couple of successful recent transactions with them. Most of the feedback I
received was not favorable so it appears the company is in
a good bit of disarray for reasons unknown. At this point, I've not gotten
a response from the owner regarding the return of our entire deposit but if
he actually comes through, I will let you all know. In the mean time, be leary
of future transactions with them. Thanks again for the feedback.
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
------------------------------
Message: 5
Date: Thu, 28 Apr 2011 10:32:22 -0400
From: "Kaye Ryan"<kr...@nfderm.com>
Subject: RE: [Histonet] IMEB
To: "Sheila Haas"<micropathl...@yahoo.com>,
<histonet@lists.utsouthwestern.edu>
Message-ID:
<dcb07c3ef84d92439e5a453d8d5b5eb066a...@exchserver.nfda.com>
Content-Type: text/plain; charset="iso-8859-1"
I usually do not respond to postings because I have seen people that may not agree with a posting
get "roasted" by others but I felt like I had to chance it with this posting. I have had
many excellent transactions with IMEB and would recommend them to anyone. Their products are
trustworthy and their prices are excellent compared to other companies that sell the same products.
I have known the owner and several of the reps for many years and have never heard of an incident
like this. I too, had to put a deposit down when I made my first order because we did not have an
established account with them. I am assuming that you did not have an account already established
since they were checking your credit status? It does take time to fill out the paper work and for
them to check your credit references. It is that way with almost any new company that you place a
large order with. In this day and time "trust" is a hard thing to come by on a new
account. It is terrible tha!
t people that may not have used them now have a negative thought in their minds about
the company. We all have at some time something that we can find not quite to our liking
about any company and I think we need to be careful when we are saying statements like
" It appears there have only been a couple of successful recent transactions with
them". This can be interpreted by some to mean that there have been many negative
interactions so stay clear of them. Maybe a lot of their customers do not reply to
posting or do not even subscribe to the histonet.
Kaye Ryan
Histology Manager
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas
Sent: Thursday, April 28, 2011 7:17 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IMEB
Thanks to everyone for the feedback on IMEB. It appears there have only been a
couple of successful recent transactions with them. Most of the feedback I
received was not favorable so it appears the company is in
a good bit of disarray for reasons unknown. At this point, I've not gotten
a response from the owner regarding the return of our entire deposit but if
he actually comes through, I will let you all know. In the mean time, be leary
of future transactions with them. Thanks again for the feedback.
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Thu, 28 Apr 2011 09:46:09 -0500
From:<sgoe...@mirnarx.com>
Subject: RE: [Histonet] IMEB
To:<kr...@nfderm.com>,<micropathl...@yahoo.com>,
<histonet@lists.utsouthwestern.edu>
Message-ID:
<d957f2a7d21959488c492a2680f9920a224...@svrexch.asuragen.us>
Content-Type: text/plain; charset="iso-8859-1"
I agree Kaye!! IMEB has always gone above and beyond to help me with anything
I have ever needed. They even had one of their techs call and help me
troubleshoot some equipment that wasn't even theirs! I can never say enough
awesome things about Denise either!! She is AWESOME!! Keeps in contact and
tries to help out in any way she can!! I bought a used processor from them and
a few months later it had an issue. They sent someone to my lab (on their dime
by the way) who fixed it on the spot! From the time I called to the time it
was fixed was less than 2 days!! It was an old VIP and the repair tech even
made his own diagnostic equipment and repair parts! I would recommend this
company to anyone (and have!!). It is a shame that one person's bad experience
has been broadcast over the histonet for the world to see. With anything in
this world there is always going to be one person who has a bad taste in their
mouth about something. Because people always yell out the !
bad and rarely yell out the good here I go...IMEB IS A GREAT COMPANY!!!!
I will now step down off my box =) Have a Happy Thursday Histo-hotties!!
Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kaye Ryan
Sent: Thursday, April 28, 2011 9:32 AM
To: Sheila Haas; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IMEB
I usually do not respond to postings because I have seen people that may not agree with a posting
get "roasted" by others but I felt like I had to chance it with this posting. I have had
many excellent transactions with IMEB and would recommend them to anyone. Their products are
trustworthy and their prices are excellent compared to other companies that sell the same products.
I have known the owner and several of the reps for many years and have never heard of an incident
like this. I too, had to put a deposit down when I made my first order because we did not have an
established account with them. I am assuming that you did not have an account already established
since they were checking your credit status? It does take time to fill out the paper work and for
them to check your credit references. It is that way with almost any new company that you place a
large order with. In this day and time "trust" is a hard thing to come by on a new
account. It is terrible tha!
t people that may not have used them now have a negative thought in their minds about
the company. We all have at some time something that we can find not quite to our liking
about any company and I think we need to be careful when we are saying statements like
" It appears there have only been a couple of successful recent transactions with
them". This can be interpreted by some to mean that there have been many negative
interactions so stay clear of them. Maybe a lot of their customers do not reply to
posting or do not even subscribe to the histonet.
Kaye Ryan
Histology Manager
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas
Sent: Thursday, April 28, 2011 7:17 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IMEB
Thanks to everyone for the feedback on IMEB. It appears there have only been a
couple of successful recent transactions with them. Most of the feedback I
received was not favorable so it appears the company is in
a good bit of disarray for reasons unknown. At this point, I've not gotten
a response from the owner regarding the return of our entire deposit but if
he actually comes through, I will let you all know. In the mean time, be leary
of future transactions with them. Thanks again for the feedback.
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Thu, 28 Apr 2011 07:48:00 -0700 (PDT)
From: Sheila Haas<micropathl...@yahoo.com>
Subject: Re: [Histonet] IMEB
To: Kaye Ryan<kr...@nfderm.com>, histonet@lists.utsouthwestern.edu
Message-ID:<829976.69833...@web161717.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Kaye,
I was telling of my situation and asked if others had recent problems. I
received many responses with two being positive and most of the others negative
(a couple were offering suggestions on how to handle). I have heard this company
was very reputable which is why I chose to do business with them in the first
place but have since heard that recently things have been quite rocky. I do
feel, at this point, that others should be leary before ordering from this
company. I'd hate for someone else to be in the same situation as we are,
particularly during these difficult economic times (we are out a large sum of
money at this point). I also stated that if the issue is resolved, I would let
everyone know but up until now it has not been.
You are right, we did not have an account with IMEB but what was promised in the
beginning is not what played out. I have been in this field for a very long time
and realize how the accounting works but I also realize that a full refund is
not unreasonable given I jumped through every hoop they requested I jump
through. Keep in mind, restocking fees typically occur when items are already
shipped or delivered to the customer and they return them. This is not the case
here, they have both the item and our money. Again, if this issue is resolved to
my satisfaction, I will gladly let everyone know.
Sheila Haas
________________________________
From: Kaye Ryan<kr...@nfderm.com>
To: Sheila Haas<micropathl...@yahoo.com>; histonet@lists.utsouthwestern.edu
Sent: Thu, April 28, 2011 10:32:22 AM
Subject: RE: [Histonet] IMEB
I usually do not respond to postings because I have seen people that may not
agree with a posting get "roasted" by others but I felt like I had to chance it
with this posting. I have had many excellent transactions with IMEB and would
recommend them to anyone. Their products are trustworthy and their prices are
excellent compared to other companies that sell the same products. I have known
the owner and several of the reps for many years and have never heard of an
incident like this. I too, had to put a deposit down when I made my first order
because we did not have an established account with them. I am assuming that
you did not have an account already established since they were checking your
credit status? It does take time to fill out the paper work and for them to
check your credit references. It is that way with almost any new company that
you place a large order with. In this day and time "trust" is a hard thing to
come by on a new account. It is terrible that people that may not have used
them now have a negative thought in their minds about the company. We all have
at some time something that we can find not quite to our liking about any
company and I think we need to be careful when we are saying statements like "
It appears there have only been a couple of successful recent transactions with
them". This can be interpreted by some to mean that there have been many
negative interactions so stay clear of them. Maybe a lot of their customers do
not reply to posting or do not even subscribe to the histonet.
Kaye Ryan
Histology Manager
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas
Sent: Thursday, April 28, 2011 7:17 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IMEB
Thanks to everyone for the feedback on IMEB. It appears there have only been a
couple of successful recent transactions with them. Most of the feedback I
received was not favorable so it appears the company is in
a good bit of disarray for reasons unknown. At this point, I've not gotten
a response from the owner regarding the return of our entire deposit but if
he actually comes through, I will let you all know. In the mean time, be leary
of future transactions with them. Thanks again for the feedback.
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Thu, 28 Apr 2011 14:48:01 +0000
From: "Bartlett, Jeanine (CDC/OID/NCEZID)"<j...@cdc.gov>
Subject: RE: [Histonet] IMEB
To: "sgoe...@mirnarx.com"<sgoe...@mirnarx.com>, "kr...@nfderm.com"
<kr...@nfderm.com>, "micropathl...@yahoo.com"
<micropathl...@yahoo.com>, "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Message-ID:
<df1cba3d83d9a344a7d6a045188e448410ff2...@embx-cham2.cdc.gov>
Content-Type: text/plain; charset="iso-8859-1"
It would clear things up quite a bit if they would simply return her call.
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
sgoe...@mirnarx.com
Sent: Thursday, April 28, 2011 10:46 AM
To: kr...@nfderm.com; micropathl...@yahoo.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IMEB
I agree Kaye!! IMEB has always gone above and beyond to help me with anything
I have ever needed. They even had one of their techs call and help me
troubleshoot some equipment that wasn't even theirs! I can never say enough
awesome things about Denise either!! She is AWESOME!! Keeps in contact and
tries to help out in any way she can!! I bought a used processor from them and
a few months later it had an issue. They sent someone to my lab (on their dime
by the way) who fixed it on the spot! From the time I called to the time it
was fixed was less than 2 days!! It was an old VIP and the repair tech even
made his own diagnostic equipment and repair parts! I would recommend this
company to anyone (and have!!). It is a shame that one person's bad experience
has been broadcast over the histonet for the world to see. With anything in
this world there is always going to be one person who has a bad taste in their
mouth about something. Because people always yell out the !
bad and rarely yell out the good here I go...IMEB IS A GREAT COMPANY!!!!
I will now step down off my box =) Have a Happy Thursday Histo-hotties!!
Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kaye Ryan
Sent: Thursday, April 28, 2011 9:32 AM
To: Sheila Haas; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IMEB
I usually do not respond to postings because I have seen people that may not agree with a posting
get "roasted" by others but I felt like I had to chance it with this posting. I have had
many excellent transactions with IMEB and would recommend them to anyone. Their products are
trustworthy and their prices are excellent compared to other companies that sell the same products.
I have known the owner and several of the reps for many years and have never heard of an incident
like this. I too, had to put a deposit down when I made my first order because we did not have an
established account with them. I am assuming that you did not have an account already established
since they were checking your credit status? It does take time to fill out the paper work and for
them to check your credit references. It is that way with almost any new company that you place a
large order with. In this day and time "trust" is a hard thing to come by on a new
account. It is terrible tha!
t people that may not have used them now have a negative thought in their minds about
the company. We all have at some time something that we can find not quite to our liking
about any company and I think we need to be careful when we are saying statements like
" It appears there have only been a couple of successful recent transactions with
them". This can be interpreted by some to mean that there have been many negative
interactions so stay clear of them. Maybe a lot of their customers do not reply to
posting or do not even subscribe to the histonet.
Kaye Ryan
Histology Manager
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas
Sent: Thursday, April 28, 2011 7:17 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IMEB
Thanks to everyone for the feedback on IMEB. It appears there have only been a
couple of successful recent transactions with them. Most of the feedback I
received was not favorable so it appears the company is in
a good bit of disarray for reasons unknown. At this point, I've not gotten
a response from the owner regarding the return of our entire deposit but if
he actually comes through, I will let you all know. In the mean time, be leary
of future transactions with them. Thanks again for the feedback.
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Thu, 28 Apr 2011 07:51:39 -0700 (PDT)
From: Rene J Buesa<rjbu...@yahoo.com>
Subject: RE: [Histonet] IMEB
To: Sheila Haas<micropathl...@yahoo.com>,
histonet@lists.utsouthwestern.edu, Kaye Ryan<kr...@nfderm.com>
Message-ID:<119316.93664...@web65711.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Kaye:
In no way this posting is aimed at "roasting" yours, not at all, it is just to
explain MY philosophy when participating in HistoNet.
When I read a posting first I consider if I have something useful to contribute and if
that is the case I post my answer and I really do not care if it will follow a
"consensus", it is my answer based on my experience and from the start I
consider that I may be wrong or that others' answers will reflect reality in a better way.
The fact that you may have a good commercial experience with IMEB in absolutely
no way excludes the possibility that others' experiences could have been
disastrous.
Additionally, it does not really matter how others have related to this company
if the one posting the initial query has had a bad experience.
Summing up do as I do: post your answer if you consider will help, and
absolutely disregard what others may think of it, and never try to impose your
criterion.
René J.
--- On Thu, 4/28/11, Kaye Ryan<kr...@nfderm.com> wrote:
From: Kaye Ryan<kr...@nfderm.com>
Subject: RE: [Histonet] IMEB
To: "Sheila Haas"<micropathl...@yahoo.com>, histonet@lists.utsouthwestern.edu
Date: Thursday, April 28, 2011, 10:32 AM
I usually do not respond to postings because I have seen people that may not agree with a posting
get "roasted" by others but I felt like I had to chance it with this posting. I have had
many excellent transactions with IMEB and would recommend them to anyone. Their products are
trustworthy and their prices are excellent compared to other companies that sell the same products.
I have known the owner and several of the reps for many years and have never heard of an incident
like this. I too, had to put a deposit down when I made my first order because we did not have an
established account with them. I am assuming that you did not have an account already established
since they were checking your credit status? It does take time to fill out the paper work and for
them to check your credit references. It is that way with almost any new company that you place a
large order with. In this day and time "trust" is a hard thing to come by on a new
account. It is terrible that people that may not have used them now have a negative
thought in their minds about the company. We all have at some time something that we can
find not quite to our liking about any company and I think we need to be careful when we
are saying statements like " It appears there have only been a couple of successful
recent transactions with them". This can be interpreted by some to mean that there
have been many negative interactions so stay clear of them. Maybe a lot of their
customers do not reply to posting or do not even subscribe to the histonet.
Kaye Ryan
Histology Manager
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas
Sent: Thursday, April 28, 2011 7:17 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IMEB
Thanks to everyone for the feedback on IMEB. It appears there have only been a
couple of successful recent transactions with them. Most of the feedback I
received was not favorable so it appears the company is in
a good bit of disarray for reasons unknown. At this point, I've not gotten
a response from the owner regarding the return of our entire deposit but if
he actually comes through, I will let you all know. In the mean time, be leary
of future transactions with them. Thanks again for the feedback.
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Thu, 28 Apr 2011 11:03:43 -0400
From: "Angela Bitting"<akbitt...@geisinger.edu>
Subject: [Histonet] Ventana Probes
To: "histonet"<histonet@lists.utsouthwestern.edu>
Message-ID:<4db9498f.2b7f.00c...@geisinger.edu>
Content-Type: text/plain; charset="us-ascii"
Ok. Ventana is on my last nerve with this new probe configuration of theirs.
I have a 4 ml vial of EBER Probe here in my lab. I am told that this is good
for approx 50 tests.
So at 100 ul a dispense, I need to bring the volume up to at least 15 ml
(probably 16ml). Not a soul under Ventana's employ will tell me what probe
diluent to use, or what else I am supposed to do with it. I'm ready to add
vodka.
If anyone out there has transitioned to the new EBER probe configuration, can
you give a gal a break and contact me?
Sorry for the Thursday rant....anyway Happy Lab Week everyone!!!
Angela Bitting, HT(ASCP), QIHC
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!
IMPORTANT WARNING: The information in this message (and the documents attached
to it, if any) is confidential and may be legally privileged. It is intended
solely for the addressee. Access to this message by anyone else is
unauthorized. If you are not the intended recipient, any disclosure, copying,
distribution or any action taken, or omitted to be taken, in reliance on it is
prohibited and may be unlawful. If you have received this message in error,
please delete all electronic copies of this message (and the documents attached
to it, if any), destroy any hard copies you may have created and notify me
immediately by replying to this email. Thank you.
-------------- next part --------------
BEGIN:VCARD
VERSION:2.1
X-GWTYPE:USER
FN:Bitting, Angela
TEL;WORK:570-271-6844
ORG:;Histology
EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu
N:Bitting;Angela
END:VCARD
------------------------------
Message: 11
Date: Thu, 28 Apr 2011 11:10:45 -0400
From: "Angela Bitting"<akbitt...@geisinger.edu>
Subject: Re: [Histonet] Ventana Probes
To: "Angela Bitting"<akbitt...@geisinger.edu>, "histonet"
<histonet@lists.utsouthwestern.edu>
Message-ID:<4db94b35.2b7f.00c...@geisinger.edu>
Content-Type: text/plain; charset=US-ASCII
Sorry folks, in my impaired state of mind I made a boo boo. I need 5-6 ml to do
50 tests. If the dispenses are still 100 ul each. Any thoughts?
"Angela Bitting"<akbitt...@geisinger.edu> 4/28/2011 11:03 AM>>>
Ok. Ventana is on my last nerve with this new probe configuration of theirs.
I have a 4 ml vial of EBER Probe here in my lab. I am told that this is good
for approx 50 tests.
So at 100 ul a dispense, I need to bring the volume up to at least 15 ml
(probably 16ml). Not a soul under Ventana's employ will tell me what probe
diluent to use, or what else I am supposed to do with it. I'm ready to add
vodka.
If anyone out there has transitioned to the new EBER probe configuration, can
you give a gal a break and contact me?
Sorry for the Thursday rant....anyway Happy Lab Week everyone!!!
Angela Bitting, HT(ASCP), QIHC
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!
IMPORTANT WARNING: The information in this message (and the documents attached
to it, if any) is confidential and may be legally privileged. It is intended
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unauthorized. If you are not the intended recipient, any disclosure, copying,
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prohibited and may be unlawful. If you have received this message in error,
please delete all electronic copies of this message (and the documents attached
to it, if any), destroy any hard copies you may have created and notify me
immediately by replying to this email. Thank you.
------------------------------
Message: 12
Date: Thu, 28 Apr 2011 08:13:18 -0700 (PDT)
From: Rene J Buesa<rjbu...@yahoo.com>
Subject: Re: [Histonet] Ventana Probes
To: histonet<histonet@lists.utsouthwestern.edu>, Angela Bitting
<akbitt...@geisinger.edu>
Message-ID:<433054.26360...@web65712.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Before adding vodka, take some "sips".
René J.
--- On Thu, 4/28/11, Angela Bitting<akbitt...@geisinger.edu> wrote:
From: Angela Bitting<akbitt...@geisinger.edu>
Subject: [Histonet] Ventana Probes
To: "histonet"<histonet@lists.utsouthwestern.edu>
Date: Thursday, April 28, 2011, 11:03 AM
Ok. Ventana is on my last nerve with this new probe configuration of theirs.
I have a 4 ml vial of EBER Probe here in my lab. I am told that this is good
for approx 50 tests.
So at 100 ul a dispense, I need to bring the volume up to at least 15 ml
(probably 16ml). Not a soul under Ventana's employ will tell me what probe
diluent to use, or what else I am supposed to do with it. I'm ready to add
vodka.
If anyone out there has transitioned to the new EBER probe configuration, can
you give a gal a break and contact me?
Sorry for the Thursday rant....anyway Happy Lab Week everyone!!!
Angela Bitting, HT(ASCP), QIHC
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!
IMPORTANT WARNING: The information in this message (and the documents attached
to it, if any) is confidential and may be legally privileged. It is intended
solely for the addressee. Access to this message by anyone else is
unauthorized. If you are not the intended recipient, any disclosure, copying,
distribution or any action taken, or omitted to be taken, in reliance on it is
prohibited and may be unlawful. If you have received this message in error,
please delete all electronic copies of this message (and the documents attached
to it, if any), destroy any hard copies you may have created and notify me
immediately by replying to this email. Thank you.
-----Inline Attachment Follows-----
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------------------------------
Message: 13
Date: Thu, 28 Apr 2011 08:17:16 -0700 (PDT)
From: Rene J Buesa<rjbu...@yahoo.com>
Subject: Re: [Histonet] Ventana Probes
To: Angela Bitting<akbitt...@geisinger.edu>, histonet
<histonet@lists.utsouthwestern.edu>, Angela Bitting
<akbitt...@geisinger.edu>
Message-ID:<533509.43351...@web65712.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
At 100µL per dispense, to do 50 tests you will need
50 x 100µL = 5000 µL = 5 ml
You do need to dilute it
René J.
--- On Thu, 4/28/11, Angela Bitting<akbitt...@geisinger.edu> wrote:
From: Angela Bitting<akbitt...@geisinger.edu>
Subject: Re: [Histonet] Ventana Probes
To: "Angela Bitting"<akbitt...@geisinger.edu>,
"histonet"<histonet@lists.utsouthwestern.edu>
Date: Thursday, April 28, 2011, 11:10 AM
Sorry folks, in my impaired state of mind I made a boo boo. I need 5-6 ml to do
50 tests. If the dispenses are still 100 ul each. Any thoughts?
"Angela Bitting"<akbitt...@geisinger.edu> 4/28/2011 11:03 AM>>>
Ok. Ventana is on my last nerve with this new probe configuration of theirs.
I have a 4 ml vial of EBER Probe here in my lab. I am told that this is good
for approx 50 tests.
So at 100 ul a dispense, I need to bring the volume up to at least 15 ml
(probably 16ml). Not a soul under Ventana's employ will tell me what probe
diluent to use, or what else I am supposed to do with it. I'm ready to add
vodka.
If anyone out there has transitioned to the new EBER probe configuration, can
you give a gal a break and contact me?
Sorry for the Thursday rant....anyway Happy Lab Week everyone!!!
Angela Bitting, HT(ASCP), QIHC
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!
IMPORTANT WARNING: The information in this message (and the documents attached
to it, if any) is confidential and may be legally privileged. It is intended
solely for the addressee. Access to this message by anyone else is
unauthorized. If you are not the intended recipient, any disclosure, copying,
distribution or any action taken, or omitted to be taken, in reliance on it is
prohibited and may be unlawful. If you have received this message in error,
please delete all electronic copies of this message (and the documents attached
to it, if any), destroy any hard copies you may have created and notify me
immediately by replying to this email. Thank you.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Thu, 28 Apr 2011 11:07:42 -0500
From: "Michele Wich"<mw...@7thwavelabs.com>
Subject: [Histonet] TNF alpha antibody
To:<histonet@lists.utsouthwestern.edu>
Message-ID:
<62A8156F8071C8439080D626DF8C33A60174E398@wave-mail.7thwave.local>
Content-Type: text/plain; charset="us-ascii"
Can anyone recommend a good TNF-alpha antibody for frozen mouse tissue?
Any suggestions are greatly appreciated!
------------------------------
Message: 15
Date: Thu, 28 Apr 2011 10:15:48 -0600
From: "Liz Chlipala"<l...@premierlab.com>
Subject: RE: [Histonet] TNF alpha antibody
To: "Michele Wich"<mw...@7thwavelabs.com>,
<histonet@lists.utsouthwestern.edu>
Message-ID:
<EE33BE5C905A3046A7FF8F58A64C8E4B1B376E@server.PremierLab.local>
Content-Type: text/plain; charset="us-ascii"
We have used the antibody from abcam on FFPE mouse tissue with good
success, have not tired it on frozen sections
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michele
Wich
Sent: Thursday, April 28, 2011 10:08 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] TNF alpha antibody
Can anyone recommend a good TNF-alpha antibody for frozen mouse tissue?
Any suggestions are greatly appreciated!
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 16
Date: Thu, 28 Apr 2011 10:30:28 -0600
From: "Liz Chlipala"<l...@premierlab.com>
Subject: [Histonet] TNF-alpha
To:<histonet@lists.utsouthwestern.edu>
Message-ID:
<EE33BE5C905A3046A7FF8F58A64C8E4B1B3770@server.PremierLab.local>
Content-Type: text/plain; charset="us-ascii"
I was incorrect in my earlier post, we actually use the antibody from
Sigma for TNF- alpha, I was thinking of IL-6 that's the antibody we get
from abcam
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504
------------------------------
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End of Histonet Digest, Vol 89, Issue 34
****************************************
