Sara, The same problem with air bubbles developed for me as well. I hadn't changed anything but it slowly got worse. On a hunch I changed the xylene substitute and it corrected everything. Can't say that I know why, but I my guess was that the miscibility with the mounting media was becoming worse. The shelf life was still good on the mounting media but it had been in stock for some time. I don't know if it makes any difference but I cover slipping manually. Of course, it was our first guess that someone was being inattentive while cover slipping but it became clear that that wasn't the case.
good luck, Jason On Tue, May 17, 2011 at 7:17 AM, Lees, Sara <sara.l...@covance.com> wrote: > Hi All, > > I am having horrible problems with air bubbles , can anyone help I have > changed the speed increased the mountant volume, clean it, kissed it, u > name it and nothing has worked...Can anyone help > > > Thanks Sara > > > > > ----------------------------------------------------- > Confidentiality Notice: This e-mail transmission > may contain confidential or legally privileged > information that is intended only for the individual > or entity named in the e-mail address. If you are not > the intended recipient, you are hereby notified that > any disclosure, copying, distribution, or reliance > upon the contents of this e-mail is strictly prohibited. > > If you have received this e-mail transmission in error, > please reply to the sender, so that we can arrange > for proper delivery, and then please delete the message > from your inbox. Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet