Sounds to me like you may want to check your slide lots to make sure the charge is consistent. I have purchased slides were there was variability within the back from slide to slide. Also, if you use CC1 and CC2 I would check to make sure that the solutions were not inadvertently switched, since harsher solutions tend to wreak havoc on samples. You may want to pH them as well to make sure they have broken down. Tech support will give you the pH range for each solution if you don't already know them.
I hope one of these will resolve your issue. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wben...@cua.md ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J [mjdess...@wvhcs.org] Sent: Wednesday, May 18, 2011 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana E-cadherin problem Hello Histonet, We've been having a recurring problem with E-cadherin from Ventana on Benchmark Ultra. It's spanned two different lots of antibody. Our tissues are suddenly falling off the slides. Negative controls are ok, just seems to be after antibody treatment. The protocol hasn't changed, it's the manufacturer recommended protocol. It also seems to happen to various tissue types and fixation times. We've tried drying the slides longer and it doesn't seem to help. Anyone else having problems with this antibody? Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdess...@wvhcs.org <mailto:mjdess...@wvhcs.org> | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
