Hello,
We have successfully stained ferret tissue using an antibody to Feline
Infectious Peritonitis Virus (which also cross-reacts with swine
Transmissible Gastroenteritis Virus). We buy our antibody from Custom
Monoclonals, Inc. and use heat-induced epitope retrieval (pressure cooker
and target retrieval solution). If you would like more details than this, I
can send an uncontrolled copy of our SOP to you.
Best regards,
Jan Shivers
Senior Scientist
IHC/Histology/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN 55108
612-624-7297
shive...@umn.edu
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----- Original Message -----
From: "Chakib Boussahmain" <chak_...@yahoo.com>
To: <histonet@lists.utsouthwestern.edu>
Sent: Tuesday, May 24, 2011 11:34 AM
Subject: [Histonet] Coronavirus antibody
Hi Histonetters,
We are interesting in doing immunostain on ferret tissues that have been
fixed in formalin and embedded in paraffin. I am just wondering if anyone
used this antibody in their lab, if so, can you please share with us your
staining protocol? antigen retrieval protocol as well?
Your help will be very much appreciated.
Thank you
Chakib HTL(ASCP)
Histology lab
MIT
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