Fortunate enough to purchase and use several TEM and SEM's over the
ye= ars, I always found Zeiss to have the best prices and products for
TEM and = Hitachi for the SEM's. Last one I bought was 200K plus.
Good Luck and= let me know if you know of anyone looking for an EM
tech in the dc area.
Nick(Rocky) Madary, HT/HTL(ASCP)QIHC
Joni Madary, PhD(in life)May 2= 4, 2011 12:01:20 PM,
histonet@lists.utsouthwestern.edu wrote:
Send Histonet= mailing list submissions to
histonet@lists.utsouthwestern.edu
To= subscribe or unsubscribe via the World Wide Web, visit
http://lists.uts= outhwestern.edu/mailman/listinfo/histonet
or, via email, send a message = with subject or body 'help' to
histonet-requ...@lists.utsouthwestern.edu You can reach the person
managing the list at
histonet-owner@lis= ts.utsouthwestern.edu
When replying, please edit your Subject line s= o it is more
specific
than "Re: Contents of Histonet digest..."
<= BR>Today's Topics:
1. RE: p63 (McMahon, Loralee A)
2. Re: Histone= t Digest, Vol 90, Issue 28 (bonesli...@aol.com)
3. RE: p63 (Bell, Lynne) 4. Re: Freezing bath solutions (Robert
Richmond)
5. Jones stain boo = boo (Norton, Sally)
6. Re: Re: Freezing bath solutions (mwhite@mcleodhea= lth.org)
7. IHC Control Tissues (Goins, Tresa)
8. FISH (wassan alkadh= umi)
9. AW: [Histonet] FISH (Gudrun Lang)
10. Las Vegas Position (Hal= e, Meredith)
11. RE: p63 (Dessoye, Michael J)
12. Thank you (Margaret= Blount)
13. Re: Re: Freezing bath solutions (abright@brightinstruments. com)
14. decal (Cynthia Pyse)
15. Re: decal (Rene J Buesa)
16. Pro= cessor power supply Back-up (kristen arvidson)
17. Coronavirus antibody = (Chakib Boussahmain)
18. Pathology Transmission Electron Micrscope (Vick= roy, Jim)
------------------------------------------------------ ----------------
Message: 1
Date: Mon, 23 May 2011 13:33:27 -0400 From: "McMahon, Loralee A"
Subj= ect: [Histonet] RE: p63
To: Joanne Clark ,
"histonet= @lists.utsouthwestern.edu"
Messag= e-ID:
Content-Type: text/plain; charset="us-ascii"
Bioca= re Medical
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Super= visor
Strong Memorial Hospital
Department of Surgical Pathology
(5= 85) 275-7210
________________________________________
From: histonet-= boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu= ] On Behalf Of Joanne
Clark [jcl...@pcnm.com]
Sent: Monday, May 23, 2011= 12:31 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] p= 63
I need to find a source for p63 antibody. Where does everyone pur
chase theirs from?
Joanne Clark,HT
Pathology Consultants of New M= exico
_______________________________________________
Histonet ma= iling list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwest= ern.edu/mailman/listinfo/histonet
------------------------------ Message: 2
Date: Mon, 23 May 2011 13:41:36 -0400 (EDT)
From: = bonesli...@aol.com
Subject: [Histonet] Re: Histonet Digest, Vol 90, Issu= e 28
To: histonet@lists.utsouthwestern.edu
Message-ID: <8CDE787CC3 64fab-17a0-7f...@webmail-d058.sysops.aol.com>
Content-Type: text/plai= n; charset="utf-8"
Dear Miss Margaret Blount:
Congratulatio= ns on your retirement!! May you enjoy good health
and many years doing the = things you WANT to do!!
God Bless!
-----Origi= nal Message-----
From: histonet-request
To: histonet
Sent: Mo= n, May 23, 2011 1:00 pm
Subject: Histonet Digest, Vol 90, Issue 28
Send Histonet mailing list submissions to
histonet@lists.utsouthwe= stern.edu
To subscribe or unsubscribe via the World Wide Web, visit
h= ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet
r, via email, s= end a message with subject or body 'help' to
histonet-requ...@lists.utso= uthwestern.edu
You can reach the person managing the list at
histonet= -ow...@lists.utsouthwestern.edu
When replying, please edit your Subject = line so it is more
specific
han "Re: Contents of Histonet digest..."
oday's Topics:
1. Immuno controls (Carrie Disbrow)
2. This is goo= dbye (Margaret Blount)
3. In vitro mouse embryo culture (Carmen Maria Ga= rcia Pascual)
4. Re: Immuno controls (Rene J Buesa)
5. RE: Histonet D= igest, Vol 90, Issue 27 (Barone, Carol )
6. RE: Histonet Digest, Vol 90,= Issue 27 (Barone, Carol )
7. Freezing bath solutions (Scott, Allison D) 8. p63 (Joanne Clark)
------------------------------------------ ---------------------------
Message: 1
ate: Sun, 22 May 2011 22:00:05= -0400
rom: Carrie Disbrow
ubject: [Histonet] Immu= no controls
o:
essage-ID:
ontent-Type: text/plain; charset= ="iso-8859-1"
i all,
fter consideration I disagree with the= consensus and also agree
with Curt's
athologist. More supplies and lab= or will be involved as cutting
more controls
ill be neccessay to meet t= he requests of the pathologist. The
patient tissue is
ut on two control= s slides. One slide is used as the negative
control and has
oth the pat= ient tissue and the known positive control which
should both be
egative= after the run. The other slide with the known positive
control and the
atient tissue in question is also stained with the positive control
staini= ng
ositive.
hank you!
------------------------------
Messa= ge: 2
ate: Mon, 23 May 2011 09:04:54 +0100
rom: "Margaret Blount"
ubject: [Histonet] This is goodbye
o:
essage-ID:
ontent-Type: text/plain; charset= ="US-ASCII"
To everyone in histoland,
This is my last full week= at work. From next Tuesday I will be
retiring
rom my job at Cambridge U= niversity. I have thoroughly enjoyed my
chosen
rofession as a research t= echnician specialising in histology. I
realise
hat because I have always= worked in research my experience is
somewhat
imited: I haven't been ask= ed for the same range of techniques
that you
ind in a routine clinical l= ab. However, I have enjoyed my work
and have
earnt and devised some tech= niques for a range of interesting
samples.
ow it's time to learn somethi= ng new!
I hope to spend more time with my lace pillow and learn to = do it
roperly, maybe learn to do Northamptonshire lace which I believe i s a
ariation on Bucks point. (I come originally from Northants) I have
h= ad
go at Bedfordshire and will learn more, it is very pretty. I also
pla= n
o travel; my next trip is to New Zealand in November.
I won't = go on. I just want to say this: You are all great guys
and
irls; you sha= re your experience and friendship with great
generosity,
hich I have alw= ays felt is what a scientist should do. Thank you
for
our comradeship an= d help over the years. Maybe I'll get to meet
some of
ou by chance durin= g my travels.
Best wishes to you all and a great big Thank You,
<= BR>From
Margaret
Miss Margaret Blount
Histology Manager
Metabolic Research Laboratories
Level 4 Institute of Metabolic Science<= BR>Box 289, Addenbrooke's
Hospital
Hills Road, Cambridge, CB2 0QQ
Tel 01223 769061/336079
------------------------------
Messa= ge: 3
ate: Mon, 23 May 2011 10:35:20 +0200 (CEST)
rom: "Carmen Maria = Garcia Pascual"
ubject: [Histonet] In vitro mouse= embryo culture
o: histonet@lists.utsouthwestern.edu
essage-ID: <6= 395165382carma...@uv.es>
ontent-Type: text/plain; charset="ISO-8859= -1"
Good Moorning to everyone:
I am Carmen Garcia a PhD student f= rom Valencia, we are know
interesting
n make in vitro culture of mouse = embryos to do toxicity tests and
in
ur lab we have not experience. some= one can give me some advise?
or
ecommend me some bibliography?
ow lo= ng can I have the embryos in culture?
hank you so much!!!
Best regard= s,
armen
------------------------------
Message: 4
ate:= Mon, 23 May 2011 07:48:50 -0700 (PDT)
rom: Rene J Buesa
ubject: Re: [Histonet] Immuno controls
o: Carrie Disbrow ,
"histonet@lists.utsouthwestern.edu"
essage-ID: <87688.31423...@web65714.mail.ac4.yahoo.co= m>
ontent-Type: text/plain; charset=iso-8859-1
Also Sprach Zarat= hustra!!!!
en� J.
From: Carrie Disbrow
o: histonet@lists.utsouthwestern.edu
ent: Sunday, May 22, 2011 = 10:00 PM
ubject: [Histonet] Immuno controls
i all,
fter co= nsideration I disagree with the consensus and also agree
with Curt's
at= hologist. More supplies and labor will be�
involved as�= �¿½ cutting more controls
ill be neccessay to meet the requ= ests of the pathologist. The
patient tissue is
ut on two controls slide= s. One slide is used as the negative
control and has
oth the patient ti= ssue and the known positive control which
should both be
egative after = the run. The other slide with the known positive
control and the
atient= tissue in question is also stained with the positive
control staining
= ositive.
hank you!
��ï¿= ½
���
ï¿= ½ï¿½ï¿½
� ï¿ ½ï¿½ï¿½
�ï¿= ½ï¿½
� _______________________________ ________________
istonet mailing list
isto...@lists.utsouthwestern.ed= u
ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet
----------= --------------------
Message: 5
ate: Mon, 23 May 2011 11:02:16 -0400<= BR>rom: "Barone, Carol "
ubject: [Histonet] RE: Hi= stonet Digest, Vol 90, Issue 27
o: essage-ID:
ontent-Type: text/plain; charset="us-ascii"
-----Original Me= ssage-----
rom: histonet-boun...@lists.utsouthwestern.edu
mailto:hist= onet-boun...@lists.utsouthwestern.edu] On Behalf Of
istonet-request@list= s.utsouthwestern.edu
ent: Sunday, May 22, 2011 1:01 PM
o: histonet@li= sts.utsouthwestern.edu
ubject: Histonet Digest, Vol 90, Issue 27
Send= Histonet mailing list submissions to
To subscribe or unsubscribe vi= a the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/list= info/histonet
r, via email, send a message with subject or body 'help' t= o
histonet-requ...@lists.utsouthwestern.edu
You can reach the person = managing the list at
histonet-ow...@lists.utsouthwestern.edu
When rep= lying, please edit your Subject line so it is more
specific than
Re: Con= tents of Histonet digest..."
oday's Topics:
1. to Rene J Buesa (M= axim Peshkov)
------------------------------------------------------ ---------------
Message: 1
ate: Sun, 22 May 2011 13:43:07 +0400
ro= m: Maxim Peshkov
ubject: [Histonet] to Rene J Buesao: Rene J Buesa
c: histonet@lists.utsouthwestern.e= du
essage-ID: <1585823684.20110522134...@mail.ru>
ontent-Type: = text/plain; charset=windows-1251
I am sorry, that uses histonet for pe= rsonal goals.
Dear Rene!
ast 3 my answers for you are returned back t= o me.
our e-mails I got successfully.
upport service of mail.ru wrote= that problem is at yahoo.com
server.
lease check your postmaster servic= e.
Here is my post-address:
axim Peshkov,
l. Mikhailovskaya, 90-A, 47909,
aganrog,
ussia.
Sincerely,
axim.
mailto:Maxim_71@= mail.ru
-----------------------------
_______________________= ________________________
istonet mailing list
istonet@lists.utsouthwe= stern.edu
ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet
En= d of Histonet Digest, Vol 90, Issue 27
*********************************= ******
------------------------------
Message: 6
ate: Mon, 23 = May 2011 11:15:34 -0400
rom: "Barone, Carol "
u= bject: [Histonet] RE: Histonet Digest, Vol 90, Issue 27
o:
essage-ID:
ontent-Type: text/plain; charset="us-ascii" Histonetters: Needed to
stain xenograft with "Bunny"-IgG-direct,
thatad already been in formailin two weeks (was FFPE). Normally
would do
n= a frozen 1:40? ....We have background everywhwere on first
un...suggest= ions? Neg is also shining??? Crazy researcher's! Got
to
ove them...when = will they learn to ask first?
We are diluting primary in plan two, and w= ill stain over-night at
4
egrees....but, this thing is lit-up like the S= UN!
was expecting low signal? Go figure?
----Original Message---= --
rom: histonet-boun...@lists.utsouthwestern.edu
mailto:histonet-bou= n...@lists.utsouthwestern.edu] On Behalf Of
istonet-request@lists.utsout= hwestern.edu
ent: Sunday, May 22, 2011 1:01 PM
o: histo...@lists.utso= uthwestern.edu
ubject: Histonet Digest, Vol 90, Issue 27
Send Histone= t mailing list submissions to
histonet@lists.utsouthwestern.edu
To su= bscribe or unsubscribe via the World Wide Web, visit
http://lists.utsout= hwestern.edu/mailman/listinfo/histonet
r, via email, send a message with= subject or body 'help' to
histonet-requ...@lists.utsouthwestern.edu
= You can reach the person managing the list at
histonet-owner@lists.utsou= thwestern.edu
When replying, please edit your Subject line so it is more
specific than
Re: Contents of Histonet digest..."
oday's Topics: 1. to Rene J Buesa (Maxim Peshkov)
----------------------------- ----------------------------------------
Message: 1
ate: Sun, 22 May = 2011 13:43:07 +0400
rom: Maxim Peshkov
ubject: [Hi= stonet] to Rene J Buesa
o: Rene J Buesa
c: histon= e...@lists.utsouthwestern.edu
essage-ID: <1585823684.20110522134307@mai= l.ru>
ontent-Type: text/plain; charset=windows-1251
I am sorry, = that uses histonet for personal goals.
Dear Rene!
ast 3 my answers fo= r you are returned back to me.
our e-mails I got successfully.
upport= service of mail.ru wrote that problem is at yahoo.com
server.
lease che= ck your postmaster service.
Here is my post-address:
axim Peshkov,
l. Mikhailovskaya, 90-A,
47909,
aganrog,
ussia.
Sincerely,
= axim.
mailto:maxim...@mail.ru
-----------------------------______________________________________
_________
istonet mailing lististo...@lists.utsouthwestern.edu
ttp://lists.utsouthwestern.edu/mailma= n/listinfo/histonet
End of Histonet Digest, Vol 90, Issue 27
********= *******************************
------------------------------
Me= ssage: 7
ate: Mon, 23 May 2011 11:22:24 -0500
rom: "Scott, Allison D" ubject: [Histonet] Freezing bath solutions
o:
essage-ID:
<1872B4A455B= 7974391609ad8034c79fc026df...@lbexch01.hchd.local>
ontent-Type: text/= plain; charset="us-ascii"
Hello to all in histoland. What type of solu= tions are being used
if any
o freezze your chucks for frozen sections. O= ur sister hospital is
sing methyl butane and there was a flash fire last= week. We use
the
ame solution. Any suggestions will be greatly apprecia= ted.
Allison Scott HT(ASCP)
istology Supervisor
BJ Hospital
ONF= IDENTIALITY NOTICE:
f you have received this e-mail in error, please imm= ediately
notify the
ender by return e-mail and delete this e-mail and an= y attachments
from
our computer system.
To the extent the informatio= n in this e-mail and any attachments
contain
rotected health informatio= n as defined by the Health Insurance
Portability
nd Accountability Act = of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts
160 and
64; or Chapter 181,= Texas Health and Safety Code, it is
confidential and/or
rivileged. Thi= s e-mail may also be confidential and/or privileged
under
exas law. The= e-mail is for the use of only the individual or
entity named
bove. If = you are not the intended recipient, or any authorized
epresentative of = the intended recipient, you are hereby notified
that any
eview, dissemi= nation or copying of this e-mail and its
attachments is
trictly prohibi= ted.
------------------------------
Message: 8
ate: Mon, 23 Ma= y 2011 16:31:08 +0000
rom: Joanne Clark
ubject: [Hi= stonet] p63
o: "histonet@lists.utsouthwestern.edu"
essage-ID:
<0494A7D4E8CC254EA2FB81464982E3786DCA= 7...@s10maild001n1.sh10.lan>
ontent-Type: text/plain; charset="us-asc= ii"
I need to find a source for p63 antibody. Where does everyone
purcha= se theirs
rom?
Joanne Clark,HT
athology Consultants of New Mexico ------------------------------
_________________________________= ______________
istonet mailing list
isto...@lists.utsouthwestern.edu<
BR>ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histo= net Digest, Vol 90, Issue 28
*
--------------------------= ----
Message: 3
Date: Mon, 23 May 2011 13:45:50 -0400
From: "B= ell, Lynne"
Subject: [Histonet] RE: p63
To: 'Jo= anne Clark' ,
"histonet@lists.utsouthwestern.edu"
Message-ID:
Content-Type: text/plain; char= set="us-ascii"
I believe the only place you can buy p63 is through= Biocare,
www.biocare.net.
Lynne Bell, HT (ASCP)
Histology Te= am Leader
802-371-4923
------------------------------
Message: 4
Date: Mon, 23 May 2011 14:12:37 -0400
From: Robert Ric= hmond
Subject: [Histonet] Re: Freezing bath solut= ions
To: histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset= =ISO-8859-1
Allison Scott after a flash fire with the stuff asks a= bout
alternatives to 2-methylbutane for freezing specimens.
There= is indeed a safe alternative, though I haven't had a chance
to
try it. = I've posted about it on Histonet more than once - here's
a
copy of an ea= rlier post of mine:
3M(tm) Novec(tm) Engineered Fluid HFE-7100
Th= is product belongs to a class of fluorocarbons called
"segregated hydrof= luoroethers (HFE's)"
According to various MSDS, HFE-7100 is
methyl no= nafluoroisobutyl ether
C4F9-O-CH3
It melts and freezes at -135 C, so = that it would remain liquid in
the
Histobath. (It would freeze solid in = liquid nitrogen, however.)
It boils at 60 C., and is listed as non-flamm= able. It has almost
no
vapor pressure at freezing temperatures.
It co= st around $230 a gallon a few years ago.
Bob Richmond
Samurai Pat= hologist
Knoxville TN
------------------------------
<= BR>Message: 5
Date: Mon, 23 May 2011 11:28:28 -0700
From: "Norton, Sa= lly"
Subject: [Histonet] Jones stain= boo boo
To:
Message-ID:
<1= 6e0693c7018c245959ac729fe66ede52db...@s107.childrens.sea.kids>
Conten= t-Type: text/plain; charset="us-ascii"
I accidentally put my renal= slides in the Methanimine Silver
(after
Periodic Acid) before putting t= hem in the Thiosemicarbizine.
Slides
take on a yellow hue which makes th= e H&E counterstain muddy. Is
there a
way to correct this?
Thank you,
Sally Norton
Histotech
CONFIDENTIALITY NOTICE: This e-mail message, including any attach
ments, is for the sole use of the intended recipient(s) and may
contain con= fidential and privileged information protected by law.
Any unauthorized rev= iew, use, disclosure or distribution is
prohibited. If you are not the inte= nded recipient, please contact
the sender by reply e-mail and destroy all c= opies of the original
message.
-----------------------------= -
Message: 6
Date: Mon, 23 May 2011 14:46:29 -0400
From: mwhit= e...@mcleodhealth.org
Subject: Re: [Histonet] Re: Freezing bath solutionsTo: Robert
Richmond
Cc: histonet@lists.utsouthw= estern.edu,
histonet-boun...@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset="us-ascii"
Allison and = Dr. Richmond:
We are using Novec 7000 successfully in our freezing b= ath and
have been
doing so for about 6 months with no major problems.
It does have some annoying traits ; however the safety benefits
outwei= gh
those issues , in my opinion. The issues are: a little more time
requ= ired
for specimen to freeze, tissues tending to float in the solution,
a= nd
evaporation. I worried that the evaporation would be a big problem
, = but
it has not .My techs discovered through experimentation that by
lowe= ring
the tissue slowly into the fluid, it would stop the flotation
probl= em.
Overall, I would recommend it. 3M has been very helpful to us. ( I'm
not
being paid in any way to endorse this!!) See below for 3M contac= t
information.
Additionally, through earlier Histonet inquiries, = I was told
about a
freezing bath for those of you who still use the old = Histobath
that is no
longer made. If anyone wants information about it, = It's on
www.ftssystems.com and the item is called a "Mult-Cool Bath". We
don't have
one , but it looks like it could be a suitable replacement.<= BR>
Here's our purchasing contact person at 3M:
Christina Cerecedes1-888-509-5224
Melanie S. White, MT(ASCP)
Laboratory Superv= isor, Systems/Anatomic Pathology
McLeod Regional Medical Center
Flore= nce, SC
(843) 777-2072
NOTICE: This e-mail message and al= l attachments transmitted with
it may contain legally PRIVILEGED and CO= NFIDENTIAL information
intended solely for the use of the addressee. If= the reader of
this
message is not the intended recipient, you are here= by notified
that any reading, dissemination, distribution, copying, or = other
use
of this message or its attachments is strictly prohibited. If= you
have received this message in error, please notify the sender
= immediately and/or notify the postmaster
(postmas...@mcleodhealth.org), and= delete this message and all
copies and backups thereof. Thank You.
------------------------------
Message: 7
Date: Mon, = 23 May 2011 18:56:05 +0000
From: "Goins, Tresa"
Subje= ct: [Histonet] IHC Control Tissues
To: "histo...@lists.utsouthwestern.ed= u"
Message-ID:
Content-Type: text/pla= in; charset="us-ascii"
If you haven't made up your mind yet on thi= s issue, here are a
couple of references that may - or may not - help:
<= BR>Veterinary Diagnostics:
http://www.ncbi.nlm.nih.gov/sites/entrez?
orig_db=PubMed&db=pubmed&cmd=Search&term=%22Journal%20o
f%20veterinary%20diagnostic%20investigation%20%3A%20official%20publ
ication%
20of%20the%20American%20Association%20of%20Veterinary%20Laboratory%
20Diagno
sticians%2C%20Inc%22%5BJour%5D%20AND%2020%5Bvolume%5D%20AND%20393%5
Bpage%5D= %20AND%202008%5Bpdat%5D
FDA
F.20 Quality Control:
http://www.fda.gov/downloads/MedicalDevices/DeviceRegulationandGuid
ance/Gui= danceDocuments/ucm094015.pdf
Tresa Goins
Histopathology Super= visor
Department of Livestock
Bozeman, Montana
-------= -----------------------
Message: 8
Date: Mon, 23 May 2011 12:23:5= 4 -0700 (PDT)
From: wassan alkadhumi
Subject:= [Histonet] FISH
To: h n
Message-= ID: <539226.53686...@web45202.mail.sp1.yahoo.com>
Content-Type: te= xt/plain; charset=iso-8859-1
Dear members
We jus= t started doing FISH using HER2 FISH pharma Dx Dako kit,
Code number K5331 on malignant breast tissues.
I was wondering what is the succe= ss ratio or percentage for say
a one run?? my
hospital is the firs= t in our region to start FISH
and I am the first to run it. = I have no experience with the
hybridizer but by
reading the&= nbsp;hybridizer manual and the kit insert i discover
it is user friendly. <= BR>I run the first batch (six slides
only) yesterday. Continue an= d finish it today.
only three of the slides worked.
I will star= t a new batch next week and I appreciate any help you
may offer to
avoi= d inaccurate result.
Thank you
Wassan
Histotec= hnician
Shorsh General Hospital
North of Iraq
----------------= --------------
Message: 9
Date: Mon, 23 May 2011 22:15:37 +0200From: "Gudrun Lang"
Subject: AW: [Histonet] FISH
T= o: "'wassan alkadhumi'"
Cc: histonet@lists.utsou= thwestern.edu
Message-ID: <00DDDF450F674127ACFF7D694679751A@dielangs.= at>
Hi Wassan,
you may depart the FISH procedure in a tissue-d= ependant first
part and a
probe-dependant second part.
The first part= comprises the pretreatment of the tissue to
retrieve and
permeabilises = it. The amount of pretreatment is dependant on the
fixation-duration of = the tissue.
The longer the fixation - the higher the temperature of the
buffer, the
longer the incubation time in the buffer, the longer the inc
ubation time in
the protease.
Troubleshooting in this part:
To= o low temperature of some slides because of inconsistent
temperature in
= the waterbath. Tissueslides mounted on the glass-slides in
different
hei= ghts.
Too short protease-incubation in relation to the fixation time. Us
ually
nuclei can be digested until you see the first tiny holes. Underdi
gested
nuclei are dense and look grey in the triplefilter.
If the nuc= lei are overdigested, they get bigger holes and fuzzy
borders.
Different= staining results due to variing fixation-times.
The second part= comprises the stringent wash. The temperature,
buffer-quality, duration= are probe-depending and are usually
provided in the
kit-handbook.
Troubleshooting second part:
Too high temp leads to loosing the bound = probe.
Too low temp leads to high background because of unspecifc bound
probes.
With your problem I would try to prolong the digestion in 10= min
steps,
until a good result is found.
Hint: standardization of fi= xation is the crucial point. In our
lab the
tumor-block is immediately f= ixed in a cassette as a FISH-block.
So it will
be fixed very well and wi= thstands the harsh treatment. The
disadvantage is
stronger autofluoresce= nce.
A further hint: be sure, that the gum around the coverslip is
harde= ned
before starting the hybridizer-protocol. 10 min drying time.
= I hope this helps
Gudrun Lang
-----Ursprüngliche= Nachricht-----
Von: histonet-boun...@lists.utsouthwestern.edu
[mailt= o:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von
wassan
alkad= humi
Gesendet: Montag, 23. Mai 2011 21:24
An: h n
Betreff: [Histon= et] FISH
Dear members
We just started doing FISH u= sing HER2 FISH pharma Dx Dako kit,
Code number
K5331
on malignant bre= ast tissues.
I was wondering what is the success ratio or percenta= ge for say
a one
run?? my
hospital is the first in our region&nb= sp;to start FISH
and I am the first to run it. I have no experien= ce with the
hybridizer
but by
reading the hybridizer = manual and the kit insert i discover it is
user
friendly.
I run the f= irst batch (six slides only) yesterday. Continue
and finish = it
today.
only three of the slides worked.
I will start a new batc= h next week and I appreciate any help you
may offer
to
avoid inaccura= te result.
Thank you
Wassan
Histotechnician
Shorsh General Hospital
North of Iraq
______________________________= _________________
Histonet mailing list
Histonet@lists.utsouthwestern= .edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Mo= n, 23 May 2011 16:53:16 -0500
From: "Hale, Meredith" Subject: [Histonet] Las Vegas Position
To:
Message-ID:
<6F33D8418806044682A391273399860F08374FCA@s
-irv-ex301.PathologyPartners.intranet>
Content-Type: text/plain; = charset="us-ascii"
Great opportunity for a Histotechnician= in a brand new laboratory!
We
are a 5 physician gastroenterology practi= ce located in Las Vegas,
NV
looking for a certified HT or HTL. Candidate= must meet CLIA
grossing
requirements. The candidate will be responsible= for routine
histology
duties. This is a part-time position that offers = a competitive
salary
and flexible hours. Interested applicants should co= ntact Meredith
Hale,
phone (214)596-2219 or e-mail mh...@carisls.com
Meredith Hale HT (ASCP) CM
Operations Liaison Di= rector and Education Coordinator
Caris Life Sciences
6655 North MacArthur Blvd, Irving Texas 75039
direct: 214-596-2219<= BR>
cell: 469-648-8253
fax: 214-596-7095
mh...@carisls.com ------------------------------
Message: 11
Date: Tue,= 24 May 2011 07:14:23 -0400
From: "Dessoye, Michael J"
Subject: RE: [Histonet] p63
To: "Joanne Clark" = ,
Message-ID:
Content-Type: text/p= lain; charset="iso-8859-1"
Biocare has a very good, and fairly ine= xpensive, p63 that we have
had good results with: http://biocare.net/produc
ts/antibodies/p/163/
________________________________
From: J= oanne Clark [mailto:jcl...@pcnm.com]
Sent: Mon 5/23/2011 12:31 PM
To:= histonet@lists.utsouthwestern.edu
Subject: [Histonet] p63
I need to find a source for p63 antibody. Where does everyone
purchase th= eirs from?
Joanne Clark,HT
Pathology Consultants of New Mexico
_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ = _ _ _ _
_ _ _ _ _
This email and any files transmitted with it are c= onfidential and
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If you have received this email in error = please notify the
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Any views expressed in this message are those of the individua= l
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Scannin= g of this message and addition of this footer is performed
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------------------------------
Message: 12
Date: Tue= , 24 May 2011 12:14:02 +0100
From: "Margaret Blount"
Subject: [Histonet] Thank you
To:
Message-ID:
Content-Type: text/plain; charset="US-ASCII"
Hello everyone!
Thank you all for your good wishes on my r= etirement. I knew you
were all
great people: you have just proved it big= time!
Thank you all from the bottom of my heart.
Keep smiling!
Much love
Margaret
------------------------------
Message: 13
Date: Tue, 24 = May 2011 13:03:21 +0000
From: abri...@brightinstruments.com
Subject: = Re: [Histonet] Re: Freezing bath solutions
To: mwh...@mcleodhealth.org,<
BR>histonet-boun...@lists.utsouthwestern.edu, "Robert Richmond"
Cc: Histonet
Messa= ge-ID:
<757762693-1306242203-cardhu_decombobulator_blackberry.rim.net
-1362851250-@b15.c2.bise7.blackberry>
Content-Type: text/plain; c= harset="Windows-1252"
Melanie,
In our Clini-RF rapid freezer we= have a device for keeping tissue
submerged in Novec 7000.
Regards <= BR>
Alan Bright
www.brightinstruments.com
Sent from my BlackBerry= ® wireless device
-----Original Message-----
From: mwhite@mc= leodhealth.org
Sender: histonet-boun...@lists.utsouthwestern.edu
Date= : Mon, 23 May 2011 14:46:29
To: Robert RichmondCc: ;
Subject: Re: [Histonet] Re: Freezing bath solutions
A= llison and Dr. Richmond:
We are using Novec 7000 successfully in our= freezing bath and have
been
doing so for about 6 months with no major p= roblems.
It does have some annoying traits ; however the safety bene= fits
outweigh
those issues , in my opinion. The issues are: a little mor= e time
required
for specimen to freeze, tissues tending to float in the = solution,
and
evaporation. I worried that the evaporation would be a big= problem
, but
it has not .My techs discovered through experimentation t= hat by
lowering
the tissue slowly into the fluid, it would stop the flot= ation
problem.
Overall, I would recommend it. 3M has been very helpf= ul to us.
(I'm not
being paid in any way to endorse this!!) See below fo= r 3M contact
information.
Additionally, through earlier Histonet = inquiries, I was told
about a
freezing bath for those of you who still u= se the old Histobath
that is no
longer made. If anyone wants information= about it, It's on
www.ftssystems.com and the item is called a "Mult-Coo= l Bath". We
don't have
one , but it looks like it could be a suitable re= placement.
Here's our purchasing contact person at 3M:
Christina = Cerecedes
1-888-509-5224
Melanie S. White, MT(ASCP)
Labora= tory Supervisor, Systems/Anatomic Pathology
McLeod Regional Medical Cent= er
Florence, SC
(843) 777-2072
NOTICE: This e-mail mes= sage and all attachments transmitted with
it may contain legally PRIVIL= EGED and CONFIDENTIAL information
intended solely for the use of the ad= dressee. If the reader of
this
message is not the intended recipient, y= ou are hereby notified
that any reading, dissemination, distribution, c= opying, or other
use
of this message or its attachments is strictly pro= hibited. If you
have received this message in error, please notify the = sender
immediately and/or notify the postmaster (postmaster@mcleodhealt
h.org), and delete this message and all copies and backups thereof.
Thank Y= ou.
_______________________________________________
Histonet = mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwe= stern.edu/mailman/listinfo/histonet
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Message: 14
Date: Tue, 24 May 2011 10:56:52 -0400
From: "= Cynthia Pyse"
Subject: [Histonet] decal
To: "'H= istonet'"
Message-ID: <001101cc1a= 22$d100e9d0$7302bd70$@com>
Content-Type: text/plain; charset="us-as= cii"
Hello Histonetters
What is everyone using to decal speci= mens. I haven't had to use
it in a few
years, before that I just purchas= ed formic acid and made a 10%
solution.
Just wanted to see if the criter= ia has changes over the years.
Thanks in
advance for the responses.
<= BR>
Cindy Pyse, CLT, HT (ASCP)
Laboratory/Histology Superviso= r
X-Cell Laboratories
e-mail cp...@x-celllab.com
<= BR>
------------------------------
Message: 15
Dat= e: Tue, 24 May 2011 08:50:13 -0700 (PDT)
From: Rene J Buesa
Subject: Re: [Histonet] decal
To: Cynthia Pyse , 'Histonet'
Message-ID: &= lt;259132.9447...@web65711.mail.ac4.yahoo.com>
Content-Type: text/pla= in; charset=iso-8859-1
For tissues containing bone, or bone specim= ens I always used the
commercial brand RDO.
For bone marrow biopsi= es I used EDTA (prepared in the lab).
René J.
From: Cynthia = Pyse
To: 'Histonet'
Sent: Tuesday, May 24, 2011 10:56 AM
Subject: [Histonet] decal<= BR>
Hello Histonetters
What is everyone using to decal specimens.= I haven't had to use it
in a few
years, before that I just purchased fo= rmic acid and made a 10%
solution.
Just wanted to see if the criteria ha= s changes over the years.
Thanks in
advance for the responses.
Cindy Pyse, CLT, HT (ASCP)
Laboratory/Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com
_______________________________________________
Histonet mailing l= ist
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu= /mailman/listinfo/histonet
------------------------------
Mes= sage: 16
Date: Tue, 24 May 2011 09:01:03 -0700 (PDT)
From: kristen ar= vidson
Subject: [Histonet] Processor power s= upply Back-up
To: histonet
Messag= e-ID: <2613.13896...@web65711.mail.ac4.yahoo.com>
Content-Type: te= xt/plain; charset=iso-8859-1
Does anyone have any suggestions on p= ower supply back-up for
Leica ASP 300? We are an independant lab and = are not hooked up
to back-up if we have an outage. Thought it might b= e a good idea
:)
Thanks.
------------------------------ Message: 17
Date: Tue, 24 May 2011 09:34:55 -0700 (PDT)
From:= Chakib Boussahmain
Subject: [Histonet] Coronavirus= antibody
To: histonet@lists.utsouthwestern.edu
Message-ID: <42506= 8.63659...@web161818.mail.bf1.yahoo.com>
Content-Type: text/plain; ch= arset=us-ascii
Hi Histonetters,
We are interesting in doing imm= unostain on ferret tissues that
have been fixed in formalin and embedded in= paraffin. I am just
wondering if anyone used this antibody in their lab, i= f so, can
you please share with us your staining protocol? antigen retrieva l
protocol as well?
Your help will be very much appreciated.
Thank yo= u
Chakib HTL(ASCP)
Histology lab
MIT
----------------------= --------
Message: 18
Date: Tue, 24 May 2011 11:46:17 -0500
Fro= m: "Vickroy, Jim"
Subject: [Histonet] Pathology = Transmission Electron Micrscope
To: "histonet@lists.utsouthwestern.edu"<= BR>
Message-ID:
<24A4826E8EF096 4d86bc5317306f58a55df5bec...@mmc-mail.ad.mhsil.com>
Content-Type: tex= t/plain; charset="us-ascii"
We are in the market to purchase a= new TEM. Our old Hitachi
instrument is 31 years old and is getting tired. = The use for
transmission em in pathology has somewhat diminished in our hos
pital environment. We still however use TEM routinely on all renal
biopsies= and nerve biopsies. Has anyone priced TEM scopes for
pathology usage latel= y. In the past there have not been many
options for a TEM that was for path= ology usage verses one for
research. Can anyone steer me in the right direc= tion. Of course
cost has become a major issue also so we are trying to make= the
best choice for our usage.
Thanks
James Vickroy BS, HT(A= SCP)
Surgical and Autopsy Pathology Technical Supervisor
Memorial= Medical Center
217-788-4046
________________________________ This message (including any
attachments) contains confidential
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is protected by law= . If you are not the intended recipient, you
should delete this message. An= y disclosure, copying, or
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End of Histonet Digest, Vol= 90, Issue 29
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<= /mailto:mh...@carisls.com>
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Histonet mailing list
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