What dilution do you guys use on a rabbit polyclonal Ki-67 antibody...I just did 1/500 and got a crap ton of noise in the areas where the positive staining was. There are definite negative "rivers" flowing through the samples, but even the tonsil control has this same fuzziness to it. Any suggestions?
Thanks PS-The antibody is Abcam Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
