Good morning everyone, I am curious to see what the standard protocol is on sentinel lymph node for melanomas. I need to establish a protocol at my institution and I have been reading a lot of literature and it seems to be a wide variety of thinking out there from bread loafing the lymph node first then cutting multiple H&E sections and IHC in between to bivalving. Some of the protocols call for up to as many as 46 H&E sections at 3 microns but it doesn't say where to take the sections for the IHC (S100, pan-melan cocktail). Would any of you like to share your protocols? thank you- Amy Farnan Histology Supervisor Northeast Health Albany, New York
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