I had major problems with frozen trichrome years ago and Jamie Watson from GNF, La Jolla said to fix in the Bouins and not fix in formalin and then mordant in Bouins. (duh) I am not sure why I never tried that, but when I fixed in Bouins for 10 minutes I got consistent trichrome on frozen skin sections.
Donna Harclerode, HT,HTL (ASCP) QIHC, SLS Arista Molecular Immunohistochemist 10455 Pacific Center Court San Diego, CA 92121 858-866-5421 donna.harcler...@aristamolecular.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
