Make sure that ALL your slides are properly dried in the oven and that NONE has any remnant of water underneath before going into the oven to dry. René J.
From: Jean Brinker <j.brin...@att.net> To: histonet@lists.utsouthwestern.edu Sent: Thursday, June 2, 2011 6:03 AM Subject: [Histonet] nuclear haziness We are having an issue with nuclear haziness on prostate biopsies in our small urology laboratory. It occurs randomly, appearing on some levels and not others within the same block. Another scenario is that we sometimes see it occur only in one small area of a core and the remaining tissue in the core looks beautiful. It can occur throughout a case (12 blocks) or not at all. We have checked for obvious problems, i.e. reagent contamination on the processor and stainer, ruled out temperature issues, microtome problems, etc. to no avail. I would appreciate any input/suggestions that would help to alleviate this problem. Thank you, Jean Brinker St. Louis Urological Surgeons St. Peters, MO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet