We do ours manually also (about 20 different antibodies). We use a combo of BioGenex and Dako. I like both set ups, but Dako is more expensive to perform than BioGenex. I like that you can buy the in dividual components from BioGenex instead of a whole ket from DAKO.
Dawn -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Smith, Allen Sent: Thursday, June 02, 2011 1:06 PM To: Marshall, Kimberly K Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Counter top immunos I like the Vector ABC horseradish peroxidase kits, especially with the Nova Red chromogen. Mouse primary antibodies usually give me better results than rabbit or goat primaries. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly K Sent: Thursday, June 02, 2011 9:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Counter top immunos Hello Histo folks Hope everyone is having a great week....I am writing to get some info on immuno kits for staining just by hand, like back when I first started in this field. We are a small lab in Alaska and all of our immunos are sent out of state, We are looking to do some of them here and try to save money, but don't really need a stainer. So are there any kits that are better than others? Is there anyone that does immunos this way that would give some much needed advise??? Again thanks in advance for all the imput... Kimberly _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
