When I was in the field of histology and performing IHC manually, I
fo= und the sequenza chambers to be clean, allowing for less reagent
usage, eas= y clean up(and forget one use, you can clean them and
reuse). Slide a= re vertical and use capillary action. Now I am
waiting for the next job,any= one need a tech?
Nick(Rocky) Madary, HT/HTL(ASCP)QIHC
Jun 3, 2011 12:01:25 P= M, histonet@lists.utsouthwestern.edu wrote:
Send Histonet= mailing list submissions to
histonet@lists.utsouthwestern.edu
To= subscribe or unsubscribe via the World Wide Web, visit
http://lists.uts= outhwestern.edu/mailman/listinfo/histonet
or, via email, send a message = with subject or body 'help' to
histonet-requ...@lists.utsouthwestern.edu You can reach the person
managing the list at
histonet-owner@lis= ts.utsouthwestern.edu
When replying, please edit your Subject line s= o it is more
specific
than "Re: Contents of Histonet digest..."
<= BR>Today's Topics:
1. RE: Counter top immunos (Smith, Allen)
2. l= aminin and collagen4 in cat and dog (Perry, Margaret)
3. RE: [IHCRG] lam= inin and collagen4 in cat and dog (Liz
Chlipala)
4. RE: Counter top immu= nos (hymclab)
5. Alternatives to Cytospin? (JR R)
6. RE: Counter top = Immunos... (JR R)
7. RE: Alternatives to Cytospin? (Tony Henwood)
8. = Re: RE: printed requisitions (histot...@imagesbyhopper.com)
9. Working V= entana Probe Protocols (Marc Shaeffer)
10. Re: Bone Marrow H&E Proto= cols (Dawud AlYasa)
11. Re: nuclear haziness (Jean Brinker)
12. prici= ng for EM on kidney (Della Speranza, Vinnie)
13. Counter top immunos (Ji= m Reilly)
14. Submission of tonsils to pathology (Richard Cartun)
15.= Processing Mouse Pancreas (Stoll, Kathryn)
16. Re: Submission of tonsil= s to pathology (Rene J Buesa)
17. Re: Processing Mouse Pancreas (Rene J = Buesa)
18. RE: Submission of tonsils to pathology (Horn, Hazel V)
19.= Submission of tonsils to Pathology - #2 (Richard Cartun)
------ ----------------------------------------------------------------
Mes= sage: 1
Date: Thu, 2 Jun 2011 18:05:30 +0000
From: "Smith, Allen"
Subject: [Histonet] RE: Counter top immunos
To: = "Marshall, Kimberly K"
Cc: "histonet@lists.utsout= hwestern.edu"
Message-ID:
= Content-Type: text/plain; charset="us-ascii"
I like the Vector ABC= horseradish peroxidase kits, especially with
the Nova Red chromogen. Mouse= primary antibodies usually give me
better results than rabbit or goat prim= aries.
-----Original Message-----
From: histonet-boun...@lists.ut= southwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Beha= lf Of
Marshall, Kimberly K
Sent: Thursday, June 02, 2011 9:57 AM
To: = histonet@lists.utsouthwestern.edu
Subject: [Histonet] Counter top immuno= s
Hello Histo folks
Hope everyone is having a great week....I= am writing to get some
info
on immuno kits for staining just by hand, l= ike back when I first
started
in this field. We are a small lab in Alask= a and all of our
immunos are
sent out of state, We are looking to do som= e of them here and try
to
save money, but don't really need a stainer. S= o are there any
kits
that are better than others? Is there anyone that d= oes immunos
this way
that would give some much needed advise???
A= gain thanks in advance for all the imput...
Kimberly
____________= ___________________________________
Histonet mailing list
Histonet@li= sts.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/= histonet
------------------------------
Message: = 2
Date: Thu, 2 Jun 2011 13:05:28 -0500
From: "Perry, Margaret"
Subject: [Histonet] laminin and collagen4 in cat = and dog
To: "ih...@googlegroups.com" ,
"histo= n...@lists.utsouthwestern.edu"
Mes= sage-ID:
Content-Type: text/plain; charset="us-ascii"
We want to use = laminin and Collagen IV to sort out the origin of
some spindle cell tumors.= Specifically, malignant peripheral nerve
sheath tumors reportedly show lam= inin and collagen IV basement
membranes vs. Fibrosarcoma which does not. We= need antibodies that
will work in both cat and dog. Is there anyone who ha= s had
success with these? Do you have any recommendations for these
antibod= ies?
Margaret Perry HT(ASCP)
Dept of Veterina= ry and Biomedical services
Box 2175
South Dakota State Univer= sity
Brookings SD 57007
605-688-5638
-= -----------------------------
Message: 3
Date: Thu, 2 Jun 2011 12= :27:00 -0600
From: "Liz Chlipala"
Subject: [Hist= onet] RE: [IHCRG] laminin and collagen4 in cat and
dog
To: "Perry, Marga= ret" ,
,
Message-ID:
Content-Type: text/plain; charset="us= -ascii"
Margaret
We have used the Collagen IV listed = below on canine but not
feline, for
laminin we have not used the antibod= y from abcam on canine or
feline but
the spec sheet states it works on m= ost mammals.
Good Luck
Liz
Col= lagen IV
SouternBio
0340-01
UNLB
Goat
Laminin
abcam
ab11575
Rabbit
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
= Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308<= BR>
office (303) 682-3949
fax (303) 682-9060
www.premierl= ab.com
Ship to Address:
1567 Skyway Drive, Un= it E
Longmont, Colorado 80504
-----Original Message-----
F= rom: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On
Behalf
Of= Perry, Margaret
Sent: Thursday, June 02, 2011 12:05 PM
To: ihcrg@goo= glegroups.com; histonet@lists.utsouthwestern.edu
Subject: [IHCRG] lamini= n and collagen4 in cat and dog
We want to use laminin and Co= llagen IV to sort out the origin of
some
spindle cell tumors. Specifical= ly, malignant peripheral nerve
sheath
tumors reportedly show laminin and= collagen IV basement membranes
vs.
Fibrosarcoma which does not. We need= antibodies that will work in
both
cat and dog. Is there anyone who has = had success with these? Do
you
have any recommendations for these antibo= dies?
Margaret Perry HT(ASCP)
Dept of Veter= inary and Biomedical services
Box 2175
South Dakota State Uni= versity
Brookings SD 57007
605-688-5638
--
You received this message because you are subscribed to the Google
Grou= ps "ihcrg" group. The IHC Resource Group is a standing
committee
within = the National Society for Histotechnology.
To post to this group, sen= d email to ih...@googlegroups.com
To unsubscribe from this group, send e= mail to
ihcrg+unsubscr...@googlegroups.com
For more options, visit th= is group at
http://groups.google.com/group/ihcrg?hl=en
To conta= ct the National Society for Histotechnology, email:
hi...@nsh.org or cal= l 443.535.4060.
------------------------------
Messag= e: 4
Date: Thu, 2 Jun 2011 13:50:43 -0500
From: hymclab
Subject: [Histonet] RE: Counter top immunos
T= o: "'Smith, Allen'" , "Marshall, Kimberly K"
Cc: "histonet@lists.utsouthwestern.edu"
Message-ID:
Content-Type: text/pla= in; charset="us-ascii"
We do ours manually also (about 20 differen= t antibodies). We use
a combo of BioGenex and Dako. I like both set ups, bu= t Dako is
more expensive to perform than BioGenex. I like that you can buy the
in dividual components from BioGenex instead of a whole ket
from DAKO.<= BR>
Dawn
-----Original Message-----
From: histonet-bounces@lis= ts.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On= Behalf Of
Smith, Allen
Sent: Thursday, June 02, 2011 1:06 PM
To: Mar= shall, Kimberly K
Cc: histonet@lists.utsouthwestern.edu
Subject: [His= tonet] RE: Counter top immunos
I like the Vector ABC horseradish per= oxidase kits, especially
with the Nova Red chromogen. Mouse primary antibod= ies usually
give me better results than rabbit or goat primaries.
--= ---Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Marshall, = Kimberly K
Sent: Thursday, June 02, 2011 9:57 AM
To: histonet@lists.u= tsouthwestern.edu
Subject: [Histonet] Counter top immunos
Hello H= isto folks
Hope everyone is having a great week....I am writing to g= et some
info on immuno kits for staining just by hand, like back when I
fir= st started in this field. We are a small lab in Alaska and all
of our immun= os are sent out of state, We are looking to do some
of them here and try to save money, but don't really need a stainer.
So are there any kits
t= hat are better than others? Is there anyone that does immunos
this way that= would give some much needed advise???
Again thanks in advance for a= ll the imput...
Kimberly
________________________________________= _______
Histonet mailing list
Histonet@lists.utsouthwestern.edu
ht= tp://lists.utsouthwestern.edu/mailman/listinfo/histonet
________= _______________________________________
Histonet mailing list
Histone= t...@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listi= nfo/histonet
CONFIDENTIALITY NOTICE: This e-mail communication and a= ny
attachments may contain confidential and privileged information for
the = use of the designated recipient(s) named above. If you are
not the intended= recipient, you are hereby notified that you have
received this communicati= on in error and that any review,
disclosure, dissemination, distribution or= copying of it or its
contents is prohibited. If you have received this com= munication
in error, please notify the sender at the electronic mail addres= s
noted above and destroy all copies of this communication and any
attachme= nts. Thank you for your cooperation.
-----------------------= -------
Message: 5
Date: Thu, 2 Jun 2011 16:54:29 -0700
From: = JR R
Subject: [Histonet] Alternatives to Cyto= spin?
To:
Message-ID:
Content-Type: text/plain; charset="= iso-8859-1"
I will be collecting bronchoalveolar lavage fluid (B= ALF) from
mice and want to do do a diff-quick stain on the fluid to charact
erize the leukocyte population.
My colleagues usually use a cytospin= to concentrate cells onto a
slide but I'll be at a lab that doesn't have t= he equipment so I'm
looking for alternatives.
Could I just put a dro= p of the BALF onto a charged slide and air
dry it? Or treat the BALF like a= blood smear?
Alternatively, could I fix the cells in...ethanol mayb= e and then
cytospin them the next day when I get back to my lab?
Any= ideas will be much appreciated.
Jerry Ricks
Research ScientistUniversity of Washington
Department of Pathology
--= ----------------------------
Message: 6
Date: Thu, 2 Jun 2011 17:= 03:46 -0700
From: JR R
Subject: [Histonet]= RE: Counter top Immunos...
To:
M= essage-ID:
Content-Type: t= ext/plain; charset="iso-8859-1"
For mouse and rabbit primary a= ntibodies I like the Vector
ImmPRESS kits. They save time. For HRP substrat= es Nova Red looks
too dull to me. I have had good results with AEC and also= with
Vector ImmPACT DAB.
Jerry Ricks
Research Scientist
Unive= rsity of Washington
Department of Pathology
-----Original Message= -----
From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-b
oun...@lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly K
Sent:= Thursday, June 02, 2011 9:57 AM
To: histonet@lists.utsouthwestern.eduSubject: [Histonet] Counter
top immunos
Hello Histo folks
H= ope everyone is having a great week....I am writing to get some
info on imm= uno kits for staining just by hand, like back when I
first started in this = field. We are a small lab in Alaska and all
of our immunos are sent out of = state, We are looking to do some
of them here and try to
save money, but= don't really need a stainer. So are there any kits
that are better than= others? Is there anyone that does immunos
this way that would give some mu= ch needed advise???
Again thanks in advance for all the imput...
Kimberly
------------------------------
Message: 7
Da= te: Fri, 3 Jun 2011 00:41:23 +0000
From: Tony Henwood
Subject: RE: [Histonet] Alternatives to Cytospin?
To: "'JR R'" ,
"histonet@lists.utsouthwestern.edu"
Message-ID: <6D6BD1DE8A5571489398B392A38 a715718859...@xmdb02.nch.kids>
Content-Type: text/plain; charset="u= s-ascii"
Jerry,
Firstly, I would not recommend fixing in etha= nol since cells will
shrink and Romanowsky staining will be compromised (th= e cells
stain too darkly since they are smaller).
I would use a cent= rifuge to concentrate the cells and smear like
a blood film or if you do no= t have a centrifuge available, place
a drop of the BALF on the slide and ei= ther air dry as you
suggested (if cell count is low) or treat it like blood= (if it
looks quite opaque - lots of cells/ & other junk).
Regar= ds
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fa= x: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury = Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145,= AUSTRALIA
-----Original Message-----
From: histonet-bounces@lis= ts.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On= Behalf Of JR
R
Sent: Friday, 3 June 2011 9:54 AM
To: histonet@lists.= utsouthwestern.edu
Subject: [Histonet] Alternatives to Cytospin?
I will be collecting bronchoalveolar lavage fluid (BALF) from mice
and = want to do do a diff-quick stain on the fluid to characterize
the leukocyte= population.
My colleagues usually use a cytospin to concentrate cel= ls onto a
slide but I'll be at a lab that doesn't have the equipment so I'm
looking for alternatives.
Could I just put a drop of the BALF onto = a charged slide and air
dry it? Or treat the BALF like a blood smear?
Alternatively, could I fix the cells in...ethanol maybe and then
cytospin= them the next day when I get back to my lab?
Any ideas will be much= appreciated.
Jerry Ricks
Research Scientist
University of Was= hington
Department of Pathology
_____________________________= __________________
Histonet mailing list
Histonet@lists.utsouthwester= n.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
* *******************************************************************
********= *****
This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom
they are ad= dressed. If you are not the intended recipient, please
delete it and notify= the sender.
Views expressed in this message and any attachments are= those of
the individual sender, and are not necessarily the views of The C
hildren's Hospital at Westmead
This note also confirms that this ema= il message has been virus
scanned and although no computer viruses were det= ected, The
Childrens Hospital at Westmead accepts no liability for any cons
equential damage resulting from email containing computer viruses.
*****
*******************************************************************
********= *
------------------------------
Message: 8
Date: = Thu, 02 Jun 2011 20:49:14 -0400
From: "histot...@imagesbyhopper.com"
Subject: Re: [Histonet] RE: printed requisit= ions
To: "Horn, Hazel V"
Cc: "histonet@lists= .utsouthwestern.edu"
Message-ID: <92f4768f-f009-41bb-a10c-02b799a4f...@imagesbyhopper.com>
Content-= Type: text/plain; charset=us-ascii
We currently use paper reqs, bu= t coming soon, we will be going
paperless. I am not sure I am going t= o like this... ;o). Surgical
staff will enter the "req" in the computer, we= receive the order &
the specimen and enter into our AP system.
= There are going to be growing pains!!!
Sent from my iPhone
On Jun 2, 2011, at 8:18 AM, "Horn, Hazel V" wrote= :
> We still use paper reqs.
>
> Hazel Horn
> = Hazel Horn, HT/HTL (ASCP)
> Supervisor of Autopsy/Histology/Transcrip= tion
> Arkansas Children's Hospital
> 1 Children's Way Slot 820 > Little Rock, AR 72202
>
> phone 501.364.4240
> = fax 501.364.3155
>
> visit us on the web at: www.archildrens.o= rg
>
>
> -----Original Message-----
> From: histo= net-boun...@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouth= western.edu] On Behalf Of
Rathborne, Toni
> Sent: Wednesday, June 01,= 2011 3:13 PM
> To: histonet@lists.utsouthwestern.edu
> Subject= : [Histonet] printed requisitions
>
>
> Does everyone s= till receive printed requisitions with your
surgical specimens? How many of= your OR's have a printer in each
room? Our OR has a shared printer for all= of the procedure rooms,
but would like to have a printer for each OR suite= . Our IT
department is saying there would be problems and has suggested tha t
we go paperless. I see many problems arising from this. If anyone
is doin= g this successfully, I'd be very interested in hearing how
you accomplished= it.
>
>
> Toni
>
> Regards,
> Toni = Rathborne
> Pathology Supervisor
> Somerset Medical Center
&= gt; 110 Rehill Ave.
> Somerville, NJ 08876
>
>
> >
> CONFIDENTIALITY NOTICE
> This message and any inclu= ded attachments are from Somerset
Medical Center
> and are intended o= nly for the addressee. The information
contained in this
> message is= confidential and may contain privileged,
confidential,
> proprietary= and/or trade secret information entitled to
protection and/or
> exem= ption from disclosure under applicable law. Unauthorized
forwarding,
>= ; printing, copying, distribution, or use of such information is
strictly> prohibited and may be unlawful. If you are not the
addressee, please > promptly delete this message and notify the
sender of the
delivery= error
> by e-mail or you may call Somerset Medical Center's computer
Help Desk
> at 908-685-2200, ext. 4050.
>
> Be sure to = visit Somerset Medical Center's Web site -
> www.somersetmedicalcent= er.com - for the most up-to-date news,
> event listings, health info= rmation and more.
> _______________________________________________> Histonet mailing
list
> Histonet@lists.utsouthwestern.edu
&= gt; http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
&= gt;
*******************************************************************
****
*******************************************************************
********
*******************************************************************
********
*******************************************************************
********
*******************************************************************
********
*******************************************************************
********
*******************************************************************
********
*******************************************************************
********= **********************************
> The information contained in thi= s message may be privileged and
confidential
> and protected from dis= closure. If the reader of this message is
not the
> intended recipie= nt, or an employee or agent responsible for
delivering this
> messag= e to the intended recipient, you are hereby notified that
any
> diss= emination, distribution or copying of this communication is
strictly
&g= t; prohibited. If you have received this communication in
error, please not= ify
> us immediately by replying to the message and deleting it from
your computer.
> Thank you.
>
> _______________________= ________________________
> Histonet mailing list
> Histonet@lis= ts.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listi= nfo/histonet
>
------------------------------
= Message: 9
Date: Thu, 2 Jun 2011 20:33:45 -0700
From: Marc Shaeffer <= mshaef...@cox.net>
Subject: [Histonet] Working Ventana Probe ProtocolsTo:
histonet@lists.utsouthwestern.edu
Message-ID: <20110602233345.O= BB79.737102.imail@fed1rmwml44>
Content-Type: text/plain; charset=ut= f-8
> Working Ventana Probe Protocols!
>
> Heres a = good starting point when using the Kappa/Lambda/EBER
Ventana Probes
>= on the Ultra:
> Kappa Probe
>
> -Depar 16 min
&= gt; - Enzyme Protease 2 for 4 min,
> -ISH (Kappa) Probe 4min
> = -Denature @ 85 degrees for 12min
> -Hybe 1 hour
> - 3 stringenc= y washes for 8 minutes each
> -Blue detection for 20 minutes
> = -counterstain 4 min
> Lambda and EBER Probes
>
> Sam= e as Kappa but use the ISH (Lambda and or EBER) Probe 4
minutes. Everything= else is
> the same.
> I also learned that there is no need= to dilute out Ventana's
probe as they
> are predilutes and ready to = use. The probe diluent that they
sell is just
> for using other probe= s.
> Hopefully this helps everyone running these probes that nee= d a
starting point!
I am not affiliated with ventana.
Marc S= haeffer
------------------------------
Message: 10
Date: Thu, 2 Jun 2011 21:27:38 -0700 (PDT)
From: Dawud AlYasa
Subject: Re: [Histonet] Bone Marrow H&E Protocols
To= : histonet@lists.utsouthwestern.edu
Message-ID: <726474.45849.qm@web3= 4506.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859= -1
What's up Tena!
Long time. The bone marrows will be fixe= d in B-5.
Dawud
------------------------------
Message: 11 Date: Fri, 3 Jun 2011 02:57:55 -0700 (PDT)
From: Jean Brinker
Subject: Re: [Histonet] nuclear haziness
To: Rene J Bue= sa ,
"histonet@lists.utsouthwestern.edu"
Message-ID: <50710.84775...@web180304.mail= .gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Thanks for your response. I had that thought as well, and the techs
have <= BR>adjusted their technique to make sure excess water is
drained from slide= s before
they are placed in the oven. They check them again before stai
ning.
________________________________
From: Ren= e J Buesa
To: Jean Brinker ; "his= to...@lists.utsouthwestern.edu"
= Sent: Thu, June 2, 2011 9:50:08 AM
Subject: Re: [Histonet] nuclear hazin= ess
Make sure that ALL your slides are properly dried in the ove= n and
that NONE has
any remnant of water underneath before going into t= he oven to
dry.
René J.
From: Jean Brinker
To: histonet@lists.utsouthwestern.edu
Sent: Thursday, June 2, 2011= 6:03 AM
Subject: [Histonet] nuclear haziness
We are having an is= sue with nuclear haziness on prostate biopsies
in our small
urology lab= oratory. It occurs randomly, appearing on some levels
and not ot= hers
within the same block. Another scenario is that we sometimes s= ee
it occur only
in one small area of a core and the remaining tissue i= n the core
looks
beautiful. It can occur throughout a case (12 blocks) = or not at
all. We have
checked for obvious problems, i.e. reagent conta= mination on the
processor and
stainer, ruled out temperature issue= s, microtome problems, etc.
to no avail. I
would appreciate any input/s= uggestions that would help to
alleviate this
problem.
Thank you= ,
Jean Brinker
St. Louis Urological Surgeons
St. Peters, MO
_______________________________________________
Histonet mailing listhisto...@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mail= man/listinfo/histonet
------------------------------
Message:= 12
Date: Fri, 3 Jun 2011 06:09:14 -0400
From: "Della Speranza, Vinni= e"
Subject: [Histonet] pricing for EM on kidney
To:= "histonet@lists.utsouthwestern.edu"
Message-ID:
Content-Type: text/plain; charset="us-ascii"
I've been = asked to get pricing for Electron Microscopy on kidneys
from labs that acce= pt out reach samples. we are interested in
your fee for EM only and not a c= omplete kidney work up.
In addition to pricing for kidney samples pr= ovided in
gluteraldehyde, I'll also need to know if your pricing is differe nt
if you are asked to work from a paraffin block.
thank you,
Vinnie Della Speranza, MS, HTL(ASCP)
Manager for Anatomic Pathology Ser= vices
Medical University of South Carolina
165 Ashley Avenue Suite 30= 9
Charleston, SC 29425
ph (843) 792-6353
fax (843) 792-8974
___= _____________________________________
----------= --------------------
Message: 13
Date: Fri, 3 Jun 2011 12:42:32 += 0100
From: "Jim Reilly"
Subject: [Histon= et] Counter top immunos
To:
Messa= ge-ID:
Content-Type: text/plain; charset="us-ascii"
Hello= Kimberly
We do all our immunos manually and the nearest thing to a = kit we
use is
the ImmPRESS Peroxidase Polymer Detection System or VECTAS= TAIN
ABC
System with ImmPACT DAB Peroxidase Substrate all from Vector
Laboratories. The majority of our Ab's are used on human tissue and
the
first choice for primary Ab's for us is mouse anti-human raised in
hors= e.
Cheers
Jim Reilly
Senior Histology Technician
Instit= ute Of Infection, Immunity, Inflammation College Of
Medical,
Veterinary = and Life Sciences University Of Glasgow 120 University
Place
Glasgow
= G12 8TA
Tel: +44 141 330 7573
-----------------------= -------
Message: 14
Date: Fri, 03 Jun 2011 11:55:21 -0400
From= : "Richard Cartun"
Subject: [Histonet] Submission= of tonsils to pathology
To: "Histonet"
Message-ID: <4de8cba8.7400.007...@harthosp.org>
Content-Typ= e: text/plain; charset=US-ASCII
What is your policy on the submiss= ion of tonsils to pathology for
examination (gross or microscopic)? Thank y= ou.
Richard
Richard W. Cartun, MS, PhD
Director, Histology= & Immunopathology
Director, Biospecimen Collection Programs
Assi= stant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Stree= t
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
<= BR>
------------------------------
Message: 15
Dat= e: Fri, 3 Jun 2011 11:03:27 -0500
From: "Stoll, Kathryn"
Subject: [Histonet] Processing Mouse Pancreas
To: "histonet@lists.u= tsouthwestern.edu"
Message-ID:
<110e7925e2b91945a9b79edfd0dc2b34e2a1888...@mcwmbx2.mcwcorp.net>
= Content-Type: text/plain; charset="us-ascii"
Happy Friday Everyone= ,
Does anyone have a protocol for processing mouse pancreas? We have
a V= IP6.
I am not familiar with the processing any animal tissue and do not
= know if it is similar to human.
Thanks in advance.
Kathryn St= oll, HT(ASCP)
Depatment of Pathology
Medical College of Wisconsin
= 9200 W Wisconsin Ave
Milwaukee WI 53226
414.805.1525
kst...@mcw.ed= u
------------------------------
Message: 16
Date:= Fri, 3 Jun 2011 09:10:35 -0700 (PDT)
From: Rene J Buesa
Subject: Re: [Histonet] Submission of tonsils to pathology
To: H= istonet , Richard Cartun
Message-ID: <231345.32485...@web65716.mail.ac4.yahoo.com&g= t;
Content-Type: text/plain; charset=iso-8859-1
Labelled "gross= only".
René J.
--- On Fri, 6/3/11, Richard Cartun wrote:
From: Richard Cartun <= BR>Subject: [Histonet] Submission of
tonsils to pathology
To: "Histonet" Date: Friday, June 3, 2011, 11:55 A= M
What is your policy on the submission of tonsils to pathology = for
examination (gross or microscopic)? Thank you.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology Director, Biospecimen Collection
Programs
Assistant Director, Anatom= ic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT = ; 06102
(860) 545-1596 Office
(860) 545-2204 Fax
_____= __________________________________________
Histonet mailing list
Hist= o...@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/li= stinfo/histonet
------------------------------
Message: 1= 7
Date: Fri, 3 Jun 2011 09:12:18 -0700 (PDT)
From: Rene J Buesa
Subject: Re: [Histonet] Processing Mouse Pancreas
To: = "histonet@lists.utsouthwestern.edu"
,= KathrynStoll
Message-ID: <722585.84511.qm@web65712.= mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Develop a good general protocol for animal tissue. You cannot have
"dedicated" protocols for every type of tissue.
René J.
---= On Fri, 6/3/11, Stoll, Kathryn wrote:
From: Sto= ll, Kathryn
Subject: [Histonet] Processing Mouse Pancre= as
To: "histonet@lists.utsouthwestern.edu"
Date: Friday, June 3, 2011, 12:03 PM
Happy Friday Ever= yone,
Does anyone have a protocol for processing mouse pancreas? W= e
have a VIP6.
I am not familiar with the processing any animal tissue a= nd do
not know if it is similar to human.
Thanks in advance.
Kathryn Stoll, HT(ASCP)
Depatment of Pathology
Medical College = of Wisconsin
9200 W Wisconsin Ave
Milwaukee WI 53226
414.805.1525<= BR>kst...@mcw.edu
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists= .utsouthwestern.edu/mailman/listinfo/histonet
------------------= ------------
Message: 18
Date: Fri, 3 Jun 2011 11:13:44 -0500
= From: "Horn, Hazel V"
Subject: RE: [Histonet] S= ubmission of tonsils to pathology
To: "'Rene J Buesa'" , Histonet
, Richard Cartun
Message-ID:
<25A4DE08332B19499904459F00AAACB719= 87e03...@evs1.archildrens.org>
Content-Type: text/plain; charset="i= so-8859-1"
Generally all are gross only. There are some exceptions d= epending
on patient history or if one is significantly larger than the othe r
the pathologists have the discretion to do a microscopic.
Hazel H= orn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcr= iption
Arkansas Children's Hospital
1 Children's Way Slot 820
Litt= le Rock, AR 72202
phone 501.364.4240
fax 501.364.3155
visi= t us on the web at: www.archildrens.org
-----Original Message---= --
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun= c...@lists.utsouthwestern.edu] On Behalf Of
Rene J Buesa
Sent: Friday, Ju= ne 03, 2011 11:11 AM
To: Histonet; Richard Cartun
Subject: Re: [Histo= net] Submission of tonsils to pathology
Labelled "gross only".
Re= né J.
--- On Fri, 6/3/11, Richard Cartun = wrote:
From: Richard Cartun
Subject: [= Histonet] Submission of tonsils to pathology
To: "Histonet"
Date: Friday, June 3, 2011, 11:55 AM
= What is your policy on the submission of tonsils to pathology for
examinati= on (gross or microscopic)? Thank you.
Richard
Richard W= . Cartun, MS, PhD
Director, Histology & Immunopathology
Director,= Biospecimen Collection Programs
Assistant Director, Anatomic Pathology<= BR>Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(8= 60) 545-1596 Office
(860) 545-2204 Fax
__________________= _____________________________
Histonet mailing list
histo...@lists.ut= southwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histon= et
_______________________________________________
Histonet mailing l= ist
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu= /mailman/listinfo/histonet
*****************************************
*******************************************************************
********
*******************************************************************
********
*******************************************************************
********
*******************************************************************
********
*******************************************************************
********
*******************************************************************
********
*******************************************************************
********
****************************************************************
The inf= ormation contained in this message may be privileged and
confidential
an= d protected from disclosure. If the reader of this message is
not the
i= ntended recipient, or an employee or agent responsible for
delivering this message to the intended recipient, you are hereby
notified that any
dissemination, distribution or copying of this communication is
strictly <= BR>prohibited. If you have received this communication
in error, please not= ify
us immediately by replying to the message and deleting it from
your= computer.
Thank you.
------------------------------
<= BR>Message: 19
Date: Fri, 03 Jun 2011 12:40:47 -0400
From: "Richard C= artun"
Subject: [Histonet] Submission of tonsils = to Pathology - #2
To: "Histonet"
= Message-ID: <4de8d64e.7400.007...@harthosp.org>
Content-Type: text= /plain; charset=US-ASCII
I apologize; I should have stated my ques= tion differently. Our
Children's Hospital wants to stop sending tonsils to = Pathology
unless the surgeon requests pathological examination. An article
published in Otolaryngology-Head and Neck Surgery (Nelson et al.)
concluded= that pathologic examination of tonsils is not a
cost-effective use of limi= ted health care resources. "Zero cases
of unsuspected pathology were identi= fied on planned gross
specimen evaluation (n=4186) and positive pathologi= c findings
on microscopic analysis (n=1066) were only identified in post-
transplant patients."
Have any of you exempted tonsils from submissi= on to Pathology? If
not, maybe we should.
Richard
Richard W. = Cartun, MS, PhD
Director, Histology & Immunopathology
Director, B= iospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-= 1596 Office
(860) 545-2204 Fax
------------------= ------------
_______________________________________________
Hist= onet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utso= uthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol= 91, Issue 4
***************************************
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
