I used pH 6 HEIR instead of HCl/enzyme for BrdU - it works very well on mouse and monkey intestine. Did not switch to HCl after that. But now I prefer to use EdU instead of BrdU (Click-iT kit from Invitrogen). It is more expensive, but much more sensitive, fast, easy to use and well compatible with all range of antigens(for multi-color fluorescence). Unfortunately, there are no enzymatic reaction for EdU - only fluorescence.
Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: agleiber...@cbiolabs.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of C B Sent: Wednesday, June 08, 2011 8:37 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] BRDU Is anyone using ph6 HEIR instead of HCl/enzyme digestion for BRDU staining? I've read 2 manufacturer datasheets that recommend pH 6 HEIR. I thought the routine pretreatment was HCl/enzyme digestion. We have FFPE rat intestine to stain. Any feeback is appreciated. Cindy Baranowski, HT SJTRI Atlanta, GA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
