Hello Tora, We surface decal the trimmed block, for one hour, using a commercial product called Rapid Decalcifier (Cellpath, UK). I don't know what it contains, other than Hydrochloric acid, but it works a treat. Other alternatives for fish have been Nair hair removal cream (applied to the surface for 10 mins or so) and fabric softner (dilute 1:10 in distilled water and soak for 30 min to an hour) Hope you are successful
Debbie Faichney Institute of Aquaculture University of Stirling Scotland -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tora Bardal Sent: 15 June 2011 10:16 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decal fish larvae Hi Does anyone have a suggestion for decal solution/ time for (weakly) ossified bone in fish larvae? Paraffin section tends to split due to the harder tissue, nice longitudinal section are hard to get. ------------------------------------------------------------------------ Tora Bardal Overingeniør/ Senior Engineer NTNU Senter for fiskeri og havbruk /NTNU Center of Fisheries and Aquaculture NTNU, 7491 Trondheim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The Sunday Times Scottish University of the Year 2009/2010 The University of Stirling is a charity registered in Scotland, number SC 011159. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
