Hi, I am using a mouse monoclonal antibody on PFA-fixed mouse brain slices, and the Vector mouse-on-mouse kit. My background with the provided avidin-biotyn is ridiculously high even after blocking endogenous biotyn. However, I get an overall low background using an Alexa-conjugated goat anti-mouse (with the slices pre-treated in Normal Goat Serum). The problem is that in the control sections with no primary, I still get staining of isolated cells. Does anyone have any thoughts/experience/recommendation with using an Alexa conjugated anti mouse IgG secondary on mouse tissue (with a mouse-on-mouse block)?
Many thanks in advance Iris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
