Why not save yourself some time and simply purchase the staining kit from 
Polysciences or Dorn and Hart Micredge.

Jack



On Jun 23, 2011, at 3:48 PM, Jesus Hernandez <jesus.w....@gmail.com> wrote:

> Dear all, 
> 
> Does anyone have a protocol on how to prepare Sirius Red stain with known 
> concentration. We are unsure about which concentration to use. We are trying 
> to stain collagen. The Sirius Red is in powder-form. Thank you.  
> 
> Jesse Hernandez
> University of Texas - San Antonio
> Department of Biomedical Engineering
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