Hi, I have a problem with some blocks that were prepared with Histogel. I was hoping someone else might have had a similar problem and figured it out. I took a photo of the blocks that were mads and put them in a Picassa album here: https://picasaweb.google.com/lh/photo/APO3HsIMa2_jPOEs3QRGUjhz3qi22FNPb2i5JJnBCAk?feat=directlink The long & short of it is that the blocs were prepared by the researcher for me to process. They are mouse kidneys. Now it is entirely possible for him to have goofed something up in preparing the Histogel blocks. I wasn't there when he did it, but when I looked at them before processing, they all looked fine. (Like the adjacent good one in the photo). When they were processed they were placed in the VIP right next to each other. When I went to embed them this morning all but one of the four looked fine. The one that didn't come out well looked like the Histogel had shrunk up and shriveled around the kidneys. I am sure this will be aweful to cut, and the researcher is going to have a bird over it since this happened with another project previously. I would like to have a decent explanation for him, so if anyone knows what might have happened and has suggestions I would love to hear it (yes vendors too are welcome to answer this of course!). By the way, this was processed on a rather short cycle of 15-20 min per station of graded ETOH from 70% to 100% with 3 xylene stations and 4 paraffin stations (45 min for these). It seems fine for everything else that was on the processor. Just that one Histogel block was the issue.
Thank you, Amos _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet