Candice: Quenching is usually done in IHC (immuno-histo-chemical) procedures and is intended to eliminate the reaction of the peroxidase (enzyme) present in all cells. If this step is not done the background (noise) in the form of dark color present in the final section could mask the actual IHC reaction. Go to the DAKO website and download a free IHC manual that can explain you this and any other aspect of the IHC general procedure. René J.
--- On Wed, 6/29/11, Candice Smoots <candice_cami...@yahoo.com> wrote: From: Candice Smoots <candice_cami...@yahoo.com> Subject: [Histonet] Quenching To: "Histonet" <histonet@lists.utsouthwestern.edu> Date: Wednesday, June 29, 2011, 11:20 AM Hi there my fellow histonetters! I was wondering if anyone out there can tell me the purpose of quenching? Thanks I remain yours truely, Candice Camille _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
