I continuation of same discussion , i would like to have clarification for our lab..we use ....beaded paraffin "Leica Paraplast" both for processing and embedding . Do you advice us to use different paraffin for both steps?
Paula, Please share if you get any feed back on Richard Allan product. Amita From: "Tim Higgins" <[email protected]> To: <[email protected]> Date: 07/08/2011 11:34 PM Subject: [Histonet] Re: Embedding Media Sent by: [email protected] Hi Paula, Obviously there are no "compression free" paraffin's on the market. Look for a paraffin with a higher polymer content, this makes the paraffin firmer and less likely to compress. Other factor for into section compression but paraffin is a good place to start. The higher polymer paraffin's are not as suited for processing, it is more for the embedding portion of the process. Use a paraffin with lower polymers content in the processor and higher for embedding and you will like the results. Tim Date: Fri, 8 Jul 2011 09:44:51 -0700 From: "Paula Lucas" <[email protected]> Subject: [Histonet] Embedding Media To: <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset="us-ascii" Hello, We are considering a switch to a different brand of paraffin and this is because I feel we are having too many compressions in some of our tissue sections. Currently, we use Tissue Path Paraplast, regular. I would like to get feedback from you as to what you prefer. Looking on line, Richard Allan has a product called Signature Series Paraffin that comes in a type L that offers compression-free sections and I was also hoping to get any feedback on that product. I would greatly appreciate any suggestions and thoughts. Thanks in advance, Paula _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
