I would think that you could potentially use an SOP for frozen sections in the clinical setting, such as following bloodborne pathogens standards. I would ban the use of freeze spray in the cryostat and that you may want to use a particulate respirator such as the #M N95 1860S, and other standard PPE's that you use when sectioning frozen sections in the clinical setting.
Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, July 11, 2011 9:50 AM To: histonet@lists.utsouthwestern.edu; SaroBascaramurty Subject: Re: [Histonet] Written SOP Really? Do you think that anybody is going to have an SOP for such an specific and narrow subject? Please remember that an SOP procedure is nothing other than the description of the method you use in a way that anybody else can follow it, and do the same thing always. I think that you will be better of if you describe how do you complete such an assignment. René K. --- On Mon, 7/11/11, Bascaramurty, Saro <saro.bascaramu...@nrc-cnrc.gc.ca> wrote: From: Bascaramurty, Saro <saro.bascaramu...@nrc-cnrc.gc.ca> Subject: [Histonet] Written SOP To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Date: Monday, July 11, 2011, 11:40 AM Hi, I am wondering if anyone of you have a written SOP on 'Cryosectioning of animal tissues implanted with human cells that are totally not screened for Hep B, HEP C, HIV, Mycobacterium tuberculosis and other adventitious agents' . If you have one written, would you be kind enough to share with me? Thanks in advance! Saro Bascaramurty Technical Officer Institute for Biodiagnostics National Research Council 435 Ellice Avenue, Winnipeg, Manitoba. R3B 1Y6 Tel: 204-984-7166 Fax:204-984-6978 email:saro.bascaramu...@nrc-cnrc.gc.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet