I would think that you could potentially use an SOP for frozen sections in the 
clinical setting, such as following bloodborne pathogens standards.  I would 
ban the use of freeze spray in the cryostat and that you may want to use a 
particulate respirator such as the #M N95 1860S, and other standard PPE's that 
you use when sectioning frozen sections in the clinical setting.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
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-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Monday, July 11, 2011 9:50 AM
To: histonet@lists.utsouthwestern.edu; SaroBascaramurty
Subject: Re: [Histonet] Written SOP

Really? Do you think that anybody is going to have an SOP for such an specific 
and narrow subject?
Please remember that an SOP procedure is nothing other than the description of 
the method you use in a way that anybody else can follow it, and do the same 
thing always.
I think that you will be better of if you describe how do you complete such an 
assignment.
René K.

--- On Mon, 7/11/11, Bascaramurty, Saro <saro.bascaramu...@nrc-cnrc.gc.ca> 
wrote:


From: Bascaramurty, Saro <saro.bascaramu...@nrc-cnrc.gc.ca>
Subject: [Histonet] Written SOP
To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>
Date: Monday, July 11, 2011, 11:40 AM


Hi,

I am wondering if anyone of you have a written SOP on 'Cryosectioning of animal 
tissues implanted with human cells that are totally not screened for Hep B, HEP 
C, HIV, Mycobacterium tuberculosis and other adventitious agents' . If you have 
one written, would you be kind enough to share with me?

Thanks in advance!


Saro Bascaramurty

Technical Officer
Institute for Biodiagnostics
National Research Council
435 Ellice Avenue,
Winnipeg, Manitoba. R3B 1Y6

Tel: 204-984-7166
Fax:204-984-6978
email:saro.bascaramu...@nrc-cnrc.gc.ca


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