B220 is a pretty good B-cell marker, although you're right that it's not restricted to the B-cell lineage. It's also expressed in some T-cells and NK cells, for example. Cells that are double positive for B220 and IgG or IgM are B-cells almost exclusively. CD19 is a good marker for mature B-cells, although pre-pro B cells do not express CD19. If you're looking at any organ other than bone marrow or spleen, there shouldn't be many pre-pro B cells and you can probably just use CD19.
Adam On Mon, Jul 11, 2011 at 1:37 PM, Michele Wich <mw...@7thwavelabs.com> wrote: > I am trying to find the most appropriate pan B-cell marker for IHC on > frozen mouse tissue. I know that CD45R/B220 used to be the most commonly > used, but recent publications suggest PAX5 or CD19 as more restricted to > the B-cell lineage. Does anyone have any other suggestions, or are these > generally what people are using these days? > > Thanks for any info. > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
