Amanda, Im not exactly sure which part of your machine is freezing based on your description, but, I have a leica 1510 and it has freezing issues. Everyone I have ever used does this. The bar that hold the specimen chucks ices and freezes over like crazy.. It will frezze the chuck in the bar holder solid. I have to get a hammer and beat it sometimes..lol. So, before I use my machine I wipe 100% alcohol across the bar to de-ice it and the chucks dont get stuck..Do not use so much that you lower your temp. Also, do not get on the oct or the stage because your blocks will not cut and be mush... Just wipe the areas daily with alcohol.. Hope this helps.
Nicole Tatum, HT ASCP Hi Histonetters! > > A few months back I emailed regarding a service contract through Leica for > our CM 3050S, and (unfortunately?) we chose not to purchase one. This week > we have had a serious issue with it... the specimen head and arm is > covered > in frost, and the object temperature sensor is reading ## instead of a > temp. > In any case, we called Leica and asked for a service call, but it is > extremely expensive and they couldn't give us an estimate of when they > will > be here because cryostats used for clinical applications have priority > over > those, like ours, that are used for research. Understandable, but > frustrating nonetheless. So my question is: does anyone know of a good, > reputable cryostat service technician (who is authorized by leica, if > possible) that is located in the Boston, MA area? > > Any help would be greatly appreciated! > > Thanks in advance, > Amanda > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet