Message: 9 Date: Fri, 22 Jul 2011 07:06:19 -0400
From: "histot...@imagesbyhopper.com" <histot...@imagesbyhopper.com> Subject: [Histonet] Recycled alcohol To: "Histonet@Lists. Utsouthwestern. Edu" <histonet@lists.utsouthwestern.edu> Message-ID: <a7b6c430-34ed-4666-86e5-8d2fa304f...@imagesbyhopper.com> Content-Type: text/plain; charset=us-ascii Hi Histonetters! For those who use recycled alcohol, I have a few questions. Do you use it in your automatic H&E stainers? Are you having any troubles with proper, consistent H&E staining? We are using recycled alcohol, and wish to continue to do so IF we can. We are having issues with our staining - hematoxylin isn't as purple as it should be, eosin is way too pink/red. We use recycled alcohol when deparaffinizing and running down to water. For the record, we are also using recycled xylene. After staining, we use 2 recycled 100s and two fresh 100s. I have increased the hem time, decreased the eosin time, adjusted the acid alcohol and bluing... I'm tearing my hair out trying to troubleshoot this! Can anyone help me? Thanks! Michelle We test and use our recycled alcohol as 95% alcohol - we do not sub it for the 100%. We also use recycled xylene with no issues. Nancy Schmitt HT, MLT(ASCP) Histology Coordinator NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet