Hi Mark -
We have had good results with:
Vimentin Clone V9 (CM048), Factor VIII (PAb-CP039) and pan-Cytokeratin,
Clone Lu-5 (CM043) from Biocare
CD3 C7930 from Sigma
CD79a Clone HM47/A9 (MS357), Melan A (MS799) from Thermo Scientific -
no luck with CD20.
CD117 (RB9038) from Thermo Scientific - data sheet states heat
retrieval is required, but we get good results without retrieval.
I have also found that while Melan A is excellent in canine tissue, S100
(PA1-38584, ThermoSci) works better in feline tissue - more positive hits with
higher intensity of specific staining.
Best regards,
Tresa Goins
Veterinary Diagnostic Lab
South 19th and Lincoln
Bozeman, MT 59718
406-994-6353 - phone
406-994-6344 - fax
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango
Sent: Monday, July 25, 2011 12:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Antibody suggestions for dog and cat
Could anyone suggest suituble antibodies for the following markers in dog
and cat tissue:
Vimentin, Pankeratin, CD3 and CD20, CD18, MelanA, Factor VIII
Also could use a suggestion for C-kit in Dog tissue only.
thanks
Mark
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