More likely buffered with calcium acetate.
The late Ralph Lillie advocated 2% calcium acetate as a good buffer for
formalin mixtures.
One gets buffering plus some calcium ions to help with the stabilization
of lipids.Also easy, just one weighing.
Anything Lillie recommends is on a very sound scientific footing.
Geoff
On 7/28/2011 11:28 AM, Harvey, Jennifer Lynn wrote:
I had a investigator say that he is using 10% Buffered Formalin Acetate to fix
his mouse brains. They order it from Fisher (127-09-3). It is buffered with
Sodium Acetate instead. Has anyone ever used this and why would you want to? I
have never heard of it before! It is a new one to me!
Jennifer Harvey, HT(ASCP) QIHC
Vanderbilt University Medical Center
Neuropathology Lab Supervisor
C-2309 Medical Center North
Nashville, TN 37232-2561
Phone: 615-343-0083
Fax: 615-343-7089
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcaul...@umdnj.edu
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