Years ago, we used to distinguish human fibroblasts in mouse tissue with vimentin; which stains human cells quite nicely, but does not cross to mouse. Kim
Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Carl Postenka <cposte...@hotmail.com> To: sdys...@mirnarx.com; histonet@lists.utsouthwestern.edu Sent: Tuesday, August 2, 2011 11:16 AM Subject: RE: [Histonet] Mouse vs. Human cells We've had succes identifying human cells in a mouse background using an antibody against human mitochondria (Neomarkers cat#MS-1372P). The primary is a monoclonal mouse anti-human, so we use a Dako ARK kit (#K3954) to eliminate the "mouse on mouse" background. HIER using citrate pH=6.0. H2O2 block. Primary diluted 1/100 (through ARK kit), for 20min @ R/T. -------------------------------------- Carl PostenkaLondon Regional Cancer Program > Date: Mon, 1 Aug 2011 12:55:54 -0500 > From: sdys...@mirnarx.com > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] Mouse vs. Human cells > > Hello Histo-hotties! > > Question: We are working with xenograft tumors in mice. The tumor cells > are human in origin. I am trying to come up with some kind of stain > that will stain all the mouse cells and none of the human cells. Just > to be able to determine if any of the human tumor cells are in a normal > looking say liver. I was thinking maybe Ki-67? It doesn't have to be > any specific marker, just to be able to see a really blue mouse organ, > and then if there are human cells of any type in the organ light up that > one cell. > > Thanks > > > > > > Sarah Goebel-Dysart, BA, HT(ASCP) > > Histotechnologist > > Mirna Therapeutics > > 2150 Woodward Street > > Suite 100 > > Austin, Texas 78744 > > (512)901-0900 ext. 6912 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
