That processing schedule should be fine for skin samples, we add an additional 100% alcohol step so we have three absolute steps at 1 hour each (I would remove one 95%). Thickness of your samples is also important they should be around 3mm in thickness if they are thicker than that they may not process properly. The other thing is that your solutions need to be fresh for samples to process properly.
Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of crochierest...@aol.com Sent: Tuesday, August 02, 2011 4:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processing of derm specimens I have recently had a problem with my skin specimens being "underprocessed". I use a Leica 300ASP. The schedule is as follows: 10% NBF x 2 for 1 hour ea. 80% Reagent Alcohol for 1 hour 95% Reagent Alcohol x 2 for 1 hour each 100% Reagent Alcohol for 1 hour each Xylene x 3 for 1 hour each Paraffin x 3 for 1 hour each The specimens are "mushy and swell on the ice" Any input is welcome. send response to : _scrochiere@nedlc.com_ (mailto:scrochi...@nedlc.com) thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
