Whenever I have to reembed an OCT block, I leave it out at RT for just a couple of minutes to let it that a little bit. Just take a razor blade and cut out the tissue (leaving some OCT around it); re-oreint it in a mold with RT OCT and then freeze it. Don't freeze it too fast, you want the old OCT to melt a little bit with the new OCT, that way you will have a block without any seams in it. Good luck! Kim
Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Daniela Bodemer <daniela.bode...@mcri.edu.au> To: histonet@lists.utsouthwestern.edu Sent: Tuesday, August 2, 2011 7:44 PM Subject: [Histonet] Reorienting frozen tissue Hi all, I would like to know if it is possible and how to reorient or reembedd human tissue, that has been frozen at -80C in OCT. Many thanks, Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9345 5930 T (03 9345 4116) E daniela.bode...@mcri.edu.au <mailto:firstname.surn...@mcri.edu.au> www.mcri.edu.au <http://www.mcri.edu.au/> This e-mail and any attachments to it (the "Communication") are, unless otherwise stated, confidential, may contain copyright material and is for the use only of the intended recipient. If you receive the Communication in error, please notify the sender immediately by return e-mail, delete the Communication and the return e-mail, and do not read, copy, retransmit or otherwise deal with it. Any views expressed in the Communication are those of the individual sender only, unless expressly stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any of its related entities. MCRI does not accept liability in connection with the integrity of or errors in the Communication, computer virus, data corruption, interference or delay arising from or in respect of the Communication. P Please consider the environment before printing this email ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet