I've used 10% zinc buffered formalin from Anatech (no alcohol) on mouse bones with good results. It's possible that the alcohol is denaturing your antigens of interest. Is there a reason you need to use alcoholic formalin?
Adam On Tue, Aug 16, 2011 at 7:45 AM, Liang, Frank <fli...@wellstatoc.com> wrote: > Hello, > > Does anyone have used alcoholic zinc-formalin to fix mouse tissues? We > tried a few times with this fixative for immunohistochemical staining, it > did not work, but when switched back to 4% paraformaldehyde, the > immunostaining worked. We like alcoholic zinc-formalin as it gives better > morphology. Any suggestions? > > Thanks, > > Frank > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Louise Renton > Sent: Tuesday, August 16, 2011 4:17 AM > To: Barone, Carol; Histonet > Subject: Re: [Histonet] protocol for in situ RT_PCR > > hi could you add me on too pelase? > > On Mon, Aug 15, 2011 at 11:09 PM, Barone, Carol <cbar...@nemours.org> > wrote: > > > Anyone got a hot protocol for In situ RT-PCR? Haven't done one in 5 > > years (on my Perk and Elmer)...Know there must be some great new > > reagents and kits out there...opinions? Send to cbar...@nemours.org. Thx > > CB > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > +27 11 717 2298 (tel & fax) > 073 5574456 (emergencies only) > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet