Dear Colleagues: I have just received the following e-mail from Leica Microsystems. It is evident that I was wrong and that now the tissues in the Peloris instrument go directly from the 2-propanol to the parafin wax and are not air dried. I just wanted to share this correction with you. René J.
--- On Mon, 8/15/11, mary.che...@leica-microsystems.com <mary.che...@leica-microsystems.com> wrote: From: mary.che...@leica-microsystems.com <mary.che...@leica-microsystems.com> Subject: Re: [Histonet] Peloris Rapid tissue processor To: "Rene J Buesa" <rjbu...@yahoo.com> Date: Monday, August 15, 2011, 7:03 PM Dear Rene, I read your statement on Peloris today. It is certainly not our intent to try to censor the information placed on Histonet. However, I would like to point out that there is some inaccuracy in your assessment. The inaccuracy is in the statement that tissues are subjected to "DRY HOT evaporation.....". This is simply not true. Within the xylene-free protocols of the instrument, isopropyl does act as a clearant instead of xylene. Therefore, tissue transitions from isopropyl directly into paraffin. The temperature of the 1st two paraffins are elevated to "evaporate" the isopropyl but the tissue is physically in paraffin undergoing infiltration during this simultaneous process. If you have any questions, we would be happy to answer them. I am hopeful that you will see fit to write in to Histonet with a clarifying statement. We would be happy to send you white papers on the Peloris technology if you are interested in receiving them. Best Regards, Mary Cheles, MPH, HTL, DLM (ASCP) Director, Technical Services & Education Programs Leica Microsystems Biosystems Division 1700 Leider Lane Buffalo Grove, IL 60089 847.317.5916 Office 847.236.3058 Fax www.leica-microsystems.com Rene J Buesa <rjbu...@yahoo.co m> To Sent by: histonet@lists.utsouthwestern.edu, histonet-bounces@ tahs...@brain.net.pk lists.utsouthwest cc ern.edu histol...@skm.org.pk Subject Re: [Histonet] Peloris Rapid tissue 08/13/2011 09:19 processor AM Muhammad: The only thing I dislike about the Peloris technology (it is a technology in itself) is that after the dehydration with 2-propanol, the tissues are subjected to a DRY HOT evaporation of the 2-propanol in vacuum before the infiltration step with melted paraffin. That step of drying out the tissues to eliminate the 2-propanol to "facilitate" the infiltration is the one I do not like because the tissues are exposed to a very high gradient. If you end buying the instrument I think you should run a large series of validation tests to find out if the results you obtain with the Peloris compare with what you are used to, not referring to the sectioning quality of the blocks, but to their microscopic appearance.. Peloris was developed in Australia by VisionBioSystems and later bought by Leica Microsystems. Although there were tests published by VisionBioSystems, all referred to animal tissues, and the instrument was never independently validated. Leica did not make known (published) independent validations. I for one would never subject the tissues to a hot dry desiccation before infiltration. I hope this will help you in your decision. René J. --- On Sat, 8/13/11, tahs...@brain.net.pk <tahs...@brain.net.pk> wrote: From: tahs...@brain.net.pk <tahs...@brain.net.pk> Subject: [Histonet] Peloris Rapid tissue processor To: histonet@lists.utsouthwestern.edu Cc: histol...@skm.org.pk Date: Saturday, August 13, 2011, 6:18 AM Dear All, Our lab is in the process of purchasing our automated tissue processor (Peloris Rapid tissue processor). I would really appreciate comments from anyone who really likes, or dislikes the processor that they are using. Thanks in advance!!! Muhammad Tahseen Histology Supervisor Deptt. Pathology Shaukat Khanum Cancer Hospital And Research Center Lahore. Pakistan Ph. +92 42 5180725-36 Ext 2369, Fax. +92 42 5180723 e-mail. Histology <@t> skm.org.pk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet