The un-coupled Naphthol salts (from the phosphatase reaction, dissolved in
the lipids of the tissue) degrade and cause N2 bubbles .
We solved this problem (in the 60ties!)with a post treatment after the
reaction:
After the reaction, rinse in dist water,
post fix in formalin 1:4 parts water (just dist. water) for 15 min.
(re-usable), Rinse in dist water and post-treat in a solution of 1%
Sulfanilic acid in 10% Acetic acid (re-usable) for 15 min., rinse in dist
water 4x and mount.
(we used at that time glycerin jelly and/or Aquamount)
Regards,
Markus F. Meyenhofer
Microscopy Labs
Box 338
Red Bank, NJ 07701
732 747 6228
mi...@superlink.net
----- Original Message -----
From: "Kevin Bennett" <bennett...@gmail.com>
To: <histonet@lists.utsouthwestern.edu>
Sent: Tuesday, August 16, 2011 2:48 PM
Subject: [Histonet] Aqueous mounting media
All,
Can anyone recommend a quality aqueous mounting media? I was using Aquatex
from EMD chemicals but it's been discontinued, tried Aqua-Mount from
Thermo
but show air bubbles a few days after coverslipping.
Thanks,
Kevin Bennett HT
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