Anyone have advice for how to get sections from epon blocks that are very soft?? I used a rapid embedding protocol for making epon blocks that I found online. Unfortunately, I misunderstood the instruction that I was to use araldite alone for the embedding media. I used the kit that came with the araldite (ddsa and dmp 30). I didn't actually use the steps for polymerizing recommended below. I just used the early steps for speeding up the processing. I just put my samples in at 60 degrees overnight, as usual.
WASH...............PBS..................2X5 MIN. 1%OsO4 in PBS...........................20 MIN RTEMP. WASH...............PBS..................2X5 MIN *70% ETOH...............................3 MIN 95% ETOH................................3 MIN 100% ETOH...............................2X5 MIN PROPYLENE OXIDE.........................2X5 MIN 1:1 P.O.:ARALDITE 502...................30 MIN (REMOVE Caps) 1:3 " " ...................20 MIN PURE ARALDITE 502.......................10 MIN EMBED CURE IN 75C OVEN FOR 50 MIN. PLACE IN 95C OVEN FOR 50 MIN. PLACE IN FREEZER AND LET COOL 10 MIN. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
