I would like to give input on this problem. We had a similar situation. The investigators couldn't flush the fecal material before fixing-destroyed the treated area of interest. After fixing, you couldn't flush the material out. We ended up manually "picking out the material" before embedding (messy and not fun!). (P.S. Food was withheld from the rats before euthanization).
If anyone has a suggestion, we would greatly appreciate hearing about it. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Thursday, August 18, 2011 3:13 PM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Histonet Digest, Vol 93, Issue 24 Has she tried opening the colons and allowing them to fix and then gently flushing the fecal matter out before processing? Andi On Aug 18, 2011, at 10:34 AM, Jennifer Sipes wrote: > Hi everyone! > > First let me thank EVERYONE for the huge response on the H&E stain on frozen tissue!!! My slides turned out great! > > Now, I've got a question that I'm thinking will stump you as much as it's stumped a co-worker and I! She is doing a study on the mucus membrane in the intestinal tract(cecum and colon mainly) of the mouse. She tried flushing the fecal matter out of the colon and cecum but lost the membrane. She decided to leave the fecal matter in, however now when I section it, it tears up my blade and gives sections that are not all that great. I was wondering if anyone knows how to acheive great sections while keeping the membrane? > > Thank you so much in advance for any and all help you can give us! > > Regards, > > Jen > > Jennifer K. Sipes, ALAT > Sr. Laboratory Technician > Johns Hopkins University > Ross 933 > 720 Rutland Avenue > Baltimore, MD 21205 > phone: 410-614-0131 > fax: 410-955-9677 > cell: 443-631-6361 > e-mail: jsip...@jhmi.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet