Hi all - a somewhat morbid question for the weekend. A student in the anatomy dept came to me with wax embedded samlpes of demineralized bone from dissection cadavers. The bone is very flinty and difficult to section. Is this perhaps due to the preseravation/embalming process that the bodies undergo? Is there something I can do to alleviate this problem prior to processing?
I have tried dewaxing and furher demineralization, but the problem sems to be in the matrix itself-- BTW, using my usual protocol I ahv been able to section elephant tusk - so I think the prob is the bone rather than what I am doing to it best love & haev a great weekend Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet