J Histochem Cytochem. 1987 May;35(5):619-21. Application of nile blue and nile red, two fluorescent probes, for detection of lipid droplets in human skeletal muscle. Bonilla E, Prelle A. Abstract Using frozen sections from human muscle biopsies, we assessed the value of Nile blue and Nile red, two fluorescent probes, as stains for lipid droplets in normal and pathological skeletal muscle fibers. In normal muscle, lipid storage disorders, and mitochondrial myopathies, Nile blue stained the lipid droplets as yellow-gold fluorescent structures. The lipid droplets were also seen as yellow-gold fluorescent structures in Nile red-stained sections, but the outstanding feature in these preparations was the staining of the membrane network of the muscle fibers and membrane proliferations in pathological muscle as red-orange fluorescent structures. These results suggest that both Nile blue and Nile red stains are useful for visualization of lipid droplets and membrane proliferations in pathological muscle biopsies.
PMID:3559182[PubMed - indexed for MEDLINE] Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Allen Sent: Tuesday, August 23, 2011 10:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nile Red stain on Froz. muscle bx's Hi, Does anyone have a method for staining frozen skeletal muscle bx's with Nile Red &/or Nile Blue for the detection of lipid droplets. Our Neuropathologist saw the stain at a conference & would like us to try it. Thanks Sharon Allen Senior Technologist Neuropathology Lab MS435U Health Sciences Centre 825 Sherbrook St. Winnipeg, Manitoba R3A 1R9 Ph# 787-4615 ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet