Hello All, I was wondering if anyone has a good protocol for pERK staining in PFA fixed tissues. I have tried several protocols (including Antigen retrieval using trypsin and dnase) but have been unsuccessful so far. I am trying to identify pERK in 10uM sections that have been fixed in 4% PFA for 1 hour. If anyone has any insight, I would really appreciate it!
Thanks in advance! Best, Mehlika _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
