Depending on the experiment. Our lab uses 4% PFA for cryosections, Methanol:DMSO (4:1) for whole mount IHC, acetone/4%PFA for cell fixation -Mehlika
> Date: Wed, 31 Aug 2011 07:45:47 -0700 > From: rjbu...@yahoo.com > To: histonet@lists.utsouthwestern.edu; thomas.crow...@novartis.com > Subject: Re: [Histonet] Common Fixatives > CC: > > Do not sweat, 85% = neutral buffered formalin. The remaining 15% are about 20 > other used in such a small number of labs that do not merit consideration. > René J. > > --- On Wed, 8/31/11, Crowell, Thomas <thomas.crow...@novartis.com> wrote: > > > From: Crowell, Thomas <thomas.crow...@novartis.com> > Subject: [Histonet] Common Fixatives > To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> > Date: Wednesday, August 31, 2011, 9:44 AM > > > Hi All, > > I would like to get a list of the most common fixatives that are being used > in clinical laboratories, both in the USA as well as globally. This would be > for all types of specimens that come through your labs. > > Any help would be greatly appreciated! > Thanks, > Tom > > > Thomas Crowell > Fellow (Molecular Discovery) > Novartis Institutes for BioMedical > Research, Inc. > 45 Sidney Street > Cambridge, MA 02139 > USA > > Phone +1 617 8717460 > Fax +1 N.A. > thomas.crow...@novartis.com<mailto:thomas.crow...@novartis.com> > www.novartis.com<http://www.novartis.com/> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
