That will depend on the size of the fish. If less than 5 mm you could use what I used doing DIF of skin cases: Place the chuck on the freezing bar and add 2-3 drops of distilled water. Introduce the fish inside the drop of water close to the chuck surface, and add another drop. Using the coolant aerosol make sure that the drop is completely frozen. Proceed to cut. René J.
--- On Tue, 9/6/11, Bruijntjes, J.P. (Joost) <joost.bruijnt...@tno.triskelion.nl> wrote: From: Bruijntjes, J.P. (Joost) <joost.bruijnt...@tno.triskelion.nl> Subject: [Histonet] (no subject) To: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Date: Tuesday, September 6, 2011, 5:54 AM Hi all I have to prepare frozen slides of zebra fish, but it is not permitted to use OCT because of the presence of different polymers in the OCT compound. Is anyone of you familiar with the preparation of frozen slides without using OCT? Thanks Joost Bruijntjes Zeist Holland TNO.NL<http://www.tno.nl/> Joost Bruijntjes T +31 88 866 17 38 F +31 30 694 49 86 E joost.bruijnt...@tno.triskelion.nl<mailto:joost.bruijnt...@tno.triskelion.nl> Disclaimer<http://www.tno.nl/tno/email/> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
