Kidney works great for Napsin -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, September 21, 2011 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 94, Issue 24
Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Legal Case SOP (Ann Angelo) 2. RE: Uni-trieve, antigen retrieval for immunofluorescence (sdys...@mirnarx.com) 3. cost of an H&E (Jennifer MacDonald) 4. RE: cost of an H&E (Burton, Lynn) 5. Effect of Freezing on Unfixed GFP (Andrew Coleman) 6. Re: Tissue handling at embedding (Rene J Buesa) 7. Re: cost of an H&E (Rene J Buesa) 8. Tissue handling at embedding (Tim Higgins) 9. RE: Uni-trieve, antigen retrieval for immunofluorescence (Andrea Marion) 10. used microscope vendors (Caroline Bass) 11. C4d (Algeo, Lacie A) 12. FW: Procedure for SOS11 (elizabeth.dick...@hcahealthcare.com) 13. unstructured proteins (Perry, Margaret) 14. RE: used microscope vendors (Rathborne, Toni) 15. Napsin A controls (Sara Baldwin/mhhcc.org) 16. Re: used microscope vendors (Rene J Buesa) 17. RE: Napsin A controls (Mike Pence) 18. Paid Survey ($50) Opportunity - Digital Pathology (Tieuvi Nguyen) 19. Klotz Solution (Breeden, Sara) 20. Urgent!! Need per diem (Heckford, Karen - SMMC-SF) ---------------------------------------------------------------------- Message: 1 Date: Tue, 20 Sep 2011 13:20:14 -0400 (EDT) From: Ann Angelo <thisis...@aol.com> Subject: [Histonet] Legal Case SOP To: histonet@lists.utsouthwestern.edu Message-ID: <8ce45d0b1bf937b-2198-21...@webmail-d008.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Can anyone share their SOP regarding the handling of legal cases (I.e. if a specimen is being requested from an outside source which is not the patient). Thank you, Ann ------------------------------ Message: 2 Date: Tue, 20 Sep 2011 12:49:06 -0500 From: <sdys...@mirnarx.com> Subject: RE: [Histonet] Uni-trieve, antigen retrieval for immunofluorescence To: <amar...@uic.edu>, <histonet@lists.utsouthwestern.edu> Message-ID: <d957f2a7d21959488c492a2680f9920a296...@svrexch.asuragen.us> Content-Type: text/plain; charset="us-ascii" I actually attended a class they put on a few months ago where they used Unitrieve. It works really well. It doesn't seem to produce any kind of background and yes, it is used at much lower temps. However the retrieval process takes a little longer, but...you can put it in an even lower temp. water bath overnight, come in to work in the morning, and your slides are reading to go. Contact Zara over there and she might be able to hook you up with a sample. Good Luck!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrea Marion Sent: Tuesday, September 20, 2011 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Uni-trieve, antigen retrieval for immunofluorescence Hello all, Has anyone used the reagent 'Uni-trieve' from Innovex? It is purported to be a universal antigen retrieval solution that can be used at lower temperatures (65-70 C for cytoplasmic antigens, and 75-80 C for nuclear antigens): http://innvx.com/unitrievepage.html The company claims that the reagent is a universal retrieval solution for all antibodies and tissues (which is silly of course - how could they know?). Does anyone have any experience with the product? I am interested because I see that increased heat during antigen retrieval causes greater tissue autofluorescence during immunofluorescence stainings. My current protocol is to use 20 minutes at 90-95 C on a hot plate using sodium citrate buffer. Does anyone else either Uni-trieve or a different reagent/protocol for immunofluorescence stainings? Thanks, Andrea Andrea Marion Graduate Student University of Illinois at Chicago _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 20 Sep 2011 11:23:55 -0700 From: Jennifer MacDonald <jmacdon...@mtsac.edu> Subject: [Histonet] cost of an H&E To: histonet@lists.utsouthwestern.edu Message-ID: <of844b0dfc.3746a884-on88257911.0064ea4b-88257911.00651...@mtsac.edu> Content-Type: text/plain; charset="US-ASCII" Has anyone calculated the approximate cost of an H&E, materials only, no labor? Including the cost of the cassette, paraffin, slide, hematoxylin, eosin and staining reagents? Thanks, Jennifer MacDonald Mt. San Antonio College ------------------------------ Message: 4 Date: Tue, 20 Sep 2011 13:30:12 -0500 From: "Burton, Lynn" <lynn.bur...@illinois.gov> Subject: RE: [Histonet] cost of an H&E To: Jennifer MacDonald <jmacdon...@mtsac.edu>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <4a6e2caca1e017408eba1b9911952cc00643dd1...@il084exmbx214.illinois.gov> Content-Type: text/plain; charset="us-ascii" We calculated that to be right around $4.05 per slide. That does not include labor. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald [jmacdon...@mtsac.edu] Sent: Tuesday, September 20, 2011 1:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cost of an H&E Has anyone calculated the approximate cost of an H&E, materials only, no labor? Including the cost of the cassette, paraffin, slide, hematoxylin, eosin and staining reagents? Thanks, Jennifer MacDonald Mt. San Antonio College _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Tue, 20 Sep 2011 15:50:51 -0400 From: Andrew Coleman <andrewcoleman...@gmail.com> Subject: [Histonet] Effect of Freezing on Unfixed GFP To: histonet@lists.utsouthwestern.edu Message-ID: <cafajl_zelxrwmclxog5rxhbfpmbkoxc5vo+-_oovqvcwwmp...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 We have performed lentiviral injections with a turboGreenFluorescentProtein (tGFP) into the brains of rats for an RNAi experiment. We did not fix the tissue but froze in isopentane on dry ice and stored the tissue at -80 deg Celsius. We want to use some sections for a reference of the injection site (GFP fluorescence) relative to the brain morphology (Neurotracer staining) in order to selecitvely laser microdissect (LMD) infected cells from other sections. Our initial studies showed that fixation of sections on slides in 4% paraformaledhyde (PFA) at 4 deg C for 5 minutes provided tGFP flourescence comparable to that found in perfused tissue, but lately we see very weak or no fluorescence with this procedure, even when increasing the time. The only difference I can think of that we saw best results soon after the brains were removed (ie 1 to 2 weeks) and now we are having trouble visualizing the tGFP ~2 months of the brains being stored at -80 degrees celsius. Does anyone have experience with or an explanation of fluorescence decreasing with a frozen, unfixed GFP over time? In the future we plan on doing these experiments including a perfusion with a low conc of PFA (to preserve RNA integrity) but for now we are trying to figure out how to identify the GFP-positive cells in the animals we have already performed surgery on. Another option would anti-tGFP IHC, but we would prefer at this point to use on slide-fixed sections as a references for our LMD. Thanks! -Andrew ------------------------------ Message: 6 Date: Tue, 20 Sep 2011 12:51:45 -0700 (PDT) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] Tissue handling at embedding To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, TimothyMorken <timothy.mor...@ucsfmedctr.org> Message-ID: <1316548305.7251.yahoomailclas...@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 This is how we do it: 1- the person grossing will dictate how many pieces should go into each cassette 2- the person cassetting will check that information against the vial with the pieces and write down in the cassettes summary form the number of pieces 3- the person embedding checks the pieces in the cassette after processing against the cassettes log. If any piece is missing between any two steps, it will be looked for. Sometimes is small and is in the vial before cassetting, or small and in the retort. Any irregularity is documented in the QA log. René J. --- On Tue, 9/20/11, Morken, Timothy <timothy.mor...@ucsfmedctr.org> wrote: From: Morken, Timothy <timothy.mor...@ucsfmedctr.org> Subject: [Histonet] Tissue handling at embedding To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Date: Tuesday, September 20, 2011, 12:44 PM What procedures do you have in place to prevent tissue loss at embedding? What do you do if tissue appears to have been lost? And do you clean embedding molds before each reuse, or after one-days use (which may be many re-uses)? Thanks for all info! Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.mor...@ucsfmedctr.org<mailto:tim.mor...@ucsfmedctr.org> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 20 Sep 2011 12:53:12 -0700 (PDT) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] cost of an H&E To: histonet@lists.utsouthwestern.edu, Jennifer MacDonald <jmacdon...@mtsac.edu> Message-ID: <1316548392.93704.yahoomailclas...@web65710.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Under separate cover I am sending something I wrote about this subject. René J. --- On Tue, 9/20/11, Jennifer MacDonald <jmacdon...@mtsac.edu> wrote: From: Jennifer MacDonald <jmacdon...@mtsac.edu> Subject: [Histonet] cost of an H&E To: histonet@lists.utsouthwestern.edu Date: Tuesday, September 20, 2011, 2:23 PM Has anyone calculated the approximate cost of an H&E, materials only, no labor? Including the cost of the cassette, paraffin, slide, hematoxylin, eosin and staining reagents? Thanks, Jennifer MacDonald Mt. San Antonio College _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 20 Sep 2011 15:03:06 -0500 From: "Tim Higgins" <thigg...@cddmedical.com> Subject: [Histonet] Tissue handling at embedding To: <histonet@lists.utsouthwestern.edu> Message-ID: <001101cc77d0$50b7afa0$e001a...@cdd.loc> Content-Type: text/plain; charset="iso-8859-1" I have worked at labs that reuse molds and ones that clean between uses. Guess it all depends on what your definition of a "clean" mold is. If you have the time and space, not reusing the molds is best practice but not always practical. Thanks, Tim Message: 10 Date: Tue, 20 Sep 2011 09:44:26 -0700 From: "Morken, Timothy" <timothy.mor...@ucsfmedctr.org> Subject: [Histonet] Tissue handling at embedding To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <8d7c2d242dbd45498006b21122072bf85e2ae...@mcinfrwem003.ucsfmedicalcenter.org > Content-Type: text/plain; charset=us-ascii What procedures do you have in place to prevent tissue loss at embedding? What do you do if tissue appears to have been lost? And do you clean embedding molds before each reuse, or after one-days use (which may be many re-uses)? Thanks for all info! Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.mor...@ucsfmedctr.org<mailto:tim.mor...@ucsfmedctr.org> ------------------------------ Message: 9 Date: Tue, 20 Sep 2011 15:19:48 -0500 From: "Andrea Marion" <amar...@uic.edu> Subject: RE: [Histonet] Uni-trieve, antigen retrieval for immunofluorescence To: sdys...@mirnarx.com Cc: histonet@lists.utsouthwestern.edu, amar...@uic.edu Message-ID: <defdb03fcad8f889eb681e91977d8bee.squir...@webmail.uic.edu> Content-Type: text/plain;charset=iso-8859-1 Hi Sarah, Thanks for the info! Have you used Unitrieve for immunofluorescence? I am wondering whether I will see a reduction in the autofluorescence caused by heating? I will request a sample to try and report back to all. Andrea Andrea Marion Graduate Student University of Illinois at Chicago On Tue, September 20, 2011 12:49 pm, sdys...@mirnarx.com wrote: > I actually attended a class they put on a few months ago where they used > Unitrieve. It works really well. It doesn't seem to produce any kind > of background and yes, it is used at much lower temps. However the > retrieval process takes a little longer, but...you can put it in an even > lower temp. water bath overnight, come in to work in the morning, and > your slides are reading to go. > Contact Zara over there and she might be able to hook you up with a > sample. > Good Luck!! > > Sarah Goebel-Dysart, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrea > Marion > Sent: Tuesday, September 20, 2011 11:59 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Uni-trieve, antigen retrieval for immunofluorescence > > Hello all, > > Has anyone used the reagent 'Uni-trieve' from Innovex? It is purported > to > be a universal antigen retrieval solution that can be used at lower > temperatures (65-70 C for cytoplasmic antigens, and 75-80 C for nuclear > antigens): > > http://innvx.com/unitrievepage.html > > The company claims that the reagent is a universal retrieval solution > for > all antibodies and tissues (which is silly of course - how could they > know?). Does anyone have any experience with the product? > > I am interested because I see that increased heat during antigen > retrieval > causes greater tissue autofluorescence during immunofluorescence > stainings. My current protocol is to use 20 minutes at 90-95 C on a hot > plate using sodium citrate buffer. Does anyone else either Uni-trieve or > a > different reagent/protocol for immunofluorescence stainings? > > Thanks, > > Andrea > > Andrea Marion > Graduate Student > University of Illinois at Chicago > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 10 Date: Tue, 20 Sep 2011 17:30:26 -0400 From: Caroline Bass <ceb...@buffalo.edu> Subject: [Histonet] used microscope vendors To: histonet@lists.utsouthwestern.edu Message-ID: <49365679-58dd-48de-80d5-0355bba95...@buffalo.edu> Content-Type: text/plain; charset=us-ascii Can anyone recommend a good source for either used microscopes or an inexpensive brand? I'm looking for a basic inverted microscope for very basic cell counts, and quick looks for cell viability and health. This is very much a quick in and out scope for students to kick around and abuse. I'm purchasing a fluorescent microscope for more extensive needs. I've looked on ebay and a couple other places, but the market is inundated with scopes that it makes it difficult to figure out. Ideally, I'd love a recommendation for a basic used scope and maybe some names of reputable vendors of refurbished units. Thanks, Caroline ------------------------------ Message: 11 Date: Tue, 20 Sep 2011 16:21:05 -0700 From: "Algeo, Lacie A" <lacie.al...@providence.org> Subject: [Histonet] C4d To: <histonet@lists.utsouthwestern.edu> Message-ID: <6a73dcdb9e46d2489d4001b4001c92ae012c4...@irmexch05.irm.inhs.org> Content-Type: text/plain; charset="us-ascii" Hi Everyone, Does anyone know of a FITC C4d that is ASR or IVD? All I have been able to find is RUO. Also, is anyone using something different to show rejection in transplant kidneys? Thank you, Lacie Lacie Algeo, HTL, MP(ASCP) Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue Spokane, WA 99220 509-474-4418 FAX 509-474-2052 lacie.al...@providence.org ------------------------------ Message: 12 Date: Wed, 21 Sep 2011 05:45:14 -0500 From: <elizabeth.dick...@hcahealthcare.com> Subject: [Histonet] FW: Procedure for SOS11 To: <histonet@lists.utsouthwestern.edu> Message-ID: <0226177269dfde48a23f40d00634258b15ef6c8...@nadcwpmsgcms10.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" Elizabeth Ann Dickert Southern Pathology 2333 McCallie Ave. Chattanooga, TN. 423-493-6904 _____________________________________________ From: Dickert Elizabeth Sent: Thursday, September 15, 2011 10:35 AM To: 'histonet-requ...@lists.utsouthwestern.edu' Subject: SOX11 Anyone doing a sox11 with good results? Have Abcam antibody / Ventana equipment (ultraview detection) Elizabeth Ann Dickert Southern Pathology 2333 McCallie Ave. Chattanooga, TN. 423-493-6904 elizabeth.dick...@hcahealthcare.com ------------------------------ Message: 13 Date: Wed, 21 Sep 2011 12:55:47 +0000 From: "Perry, Margaret" <margaret.pe...@sdstate.edu> Subject: [Histonet] unstructured proteins To: "ih...@googlegroups.com" <ih...@googlegroups.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <25F4FBA34BE9D142964ECC4525B82AEE012722@SDSU-EX03.jacks.local> Content-Type: text/plain; charset="us-ascii" Have any of you worked with the unstructured proteins? Do they form the same crosslinks when fixed with formalin? Would antigen retrieval be of any use? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ------------------------------ Message: 14 Date: Wed, 21 Sep 2011 13:54:00 +0000 From: "Rathborne, Toni" <trathbo...@somerset-healthcare.com> Subject: RE: [Histonet] used microscope vendors To: "'Caroline Bass'" <ceb...@buffalo.edu>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <3ad061fe740d464fac7bf6b5cfb7570711f56...@smcmail01.somerset-healthcare.com> Content-Type: text/plain; charset="us-ascii" What part of the country are you in? -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Caroline Bass Sent: Tuesday, September 20, 2011 5:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] used microscope vendors Can anyone recommend a good source for either used microscopes or an inexpensive brand? I'm looking for a basic inverted microscope for very basic cell counts, and quick looks for cell viability and health. This is very much a quick in and out scope for students to kick around and abuse. I'm purchasing a fluorescent microscope for more extensive needs. I've looked on ebay and a couple other places, but the market is inundated with scopes that it makes it difficult to figure out. Ideally, I'd love a recommendation for a basic used scope and maybe some names of reputable vendors of refurbished units. Thanks, Caroline _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ------------------------------ Message: 15 Date: Wed, 21 Sep 2011 10:10:59 -0400 From: "Sara Baldwin/mhhcc.org" <sbald...@mhhcc.org> Subject: [Histonet] Napsin A controls To: <histonet@lists.utsouthwestern.edu> Message-ID: <OFB1EA30C6.094302F1-ON85257912.004DE8FD-85257912.004DE903@LocalDomain> Content-Type: text/plain; charset=ISO-8859-1 Hi Histonetters Are there any other controls for Napsin A other than Lung adenocarcinoma? Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. ------------------------------ Message: 16 Date: Wed, 21 Sep 2011 07:12:40 -0700 (PDT) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] used microscope vendors To: histonet@lists.utsouthwestern.edu, Caroline Bass <ceb...@buffalo.edu> Message-ID: <1316614360.28378.yahoomailclas...@web65703.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Try eBay. I have seen good instruments for sale there. René J. --- On Tue, 9/20/11, Caroline Bass <ceb...@buffalo.edu> wrote: From: Caroline Bass <ceb...@buffalo.edu> Subject: [Histonet] used microscope vendors To: histonet@lists.utsouthwestern.edu Date: Tuesday, September 20, 2011, 5:30 PM Can anyone recommend a good source for either used microscopes or an inexpensive brand? I'm looking for a basic inverted microscope for very basic cell counts, and quick looks for cell viability and health. This is very much a quick in and out scope for students to kick around and abuse. I'm purchasing a fluorescent microscope for more extensive needs. I've looked on ebay and a couple other places, but the market is inundated with scopes that it makes it difficult to figure out. Ideally, I'd love a recommendation for a basic used scope and maybe some names of reputable vendors of refurbished units. Thanks, Caroline _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Wed, 21 Sep 2011 09:15:32 -0500 From: "Mike Pence" <mpe...@grhs.net> Subject: RE: [Histonet] Napsin A controls To: "Sara Baldwin/mhhcc.org" <sbald...@mhhcc.org>, <histonet@lists.utsouthwestern.edu> Message-ID: <661949901a768e4f9cc16d8af8f2838c03974...@is-e2k3.grhs.net> Content-Type: text/plain; charset="US-ASCII" Kidney -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Wednesday, September 21, 2011 9:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Napsin A controls Hi Histonetters Are there any other controls for Napsin A other than Lung adenocarcinoma? Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Wed, 21 Sep 2011 08:22:15 -0700 From: Tieuvi Nguyen <tngu...@kaiserassociates.com> Subject: [Histonet] Paid Survey ($50) Opportunity - Digital Pathology To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <61C52EFB4F0AF8498D9ECADA6796B46620E26EDE@VA3DIAXVS3D1.RED001.local> Content-Type: text/plain; charset="us-ascii" Hi Everyone, I had sent a note out to the group about a month ago inviting participants to take part in phone interviews to discuss Digital Pathology technology. We are now following up those interviews with an online survey on the same subject. We are particularly interested in individuals that work in high volume commercial or reference labs (i.e. process more than 100,000 slides a year, and process more than 5,000 IHC slides a year). You do not have to be a current user of digital pathology to participate. The survey will begin with a few questions to determine if you qualify for the survey. If you qualify and complete the survey you will receive an honoraria in the form of a $50 American Express gift card for your participation. All answers will remain anonymous. The survey should take about 15-20 minutes to complete. You can access the survey here: http://www.keysurvey.com/survey/387885/297f/ Please feel free to contact me if you have any questions. Tieuvi Nguyen, PhD / Kaiser Associates, Inc. M +1 202 506 0775 / tngu...@kaiserassociates.com 1201 Connecticut Ave NW, 12th Floor / Washington, DC 20036 USA www.kaiserassociates.com ------------------------------ Message: 19 Date: Wed, 21 Sep 2011 10:07:31 -0600 From: "Breeden, Sara" <sbree...@nmda.nmsu.edu> Subject: [Histonet] Klotz Solution To: <histonet@lists.utsouthwestern.edu> Message-ID: <4d14f0fc9316dd41972d5f03c070908b051df...@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" I've just "googled" and "histosearched" for Klotz solution but cannot find anything that doesn't require chloral hydrate. Has anyone made a Big Breakthrough in making Klotz that can be made without that pesky controlled substance? Any help will be appreciated...who knew the recipe joined the Witness Protection Program! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ------------------------------ Message: 20 Date: Wed, 21 Sep 2011 09:29:07 -0700 From: "Heckford, Karen - SMMC-SF" <karen.heckf...@chw.edu> Subject: [Histonet] Urgent!! Need per diem To: <histonet@lists.utsouthwestern.edu> Message-ID: <9ecf174e7da83046be6ebfde009e28a388c...@chw-msg-301.chw.edu> Content-Type: text/plain; charset="us-ascii" I am looking for a Certified ASCP HistoTech. that is local to the San Francisco Bay Area. I need this person to cover me while I am out having knee surgery. I will be out 2-6 weeks probably more like 4 weeks starting October 5th. But need somebody by October 3rd to do some training. You will need to know IHC and Special stains and be able to work independently because I am the Chief, Cook and Bottle washer here. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 94, Issue 24 **************************************** -------------------------------------------------------- Confidentiality Notice: The information contained in this e-mail is intended solely for the person(s) to whom it is addressed. This information is the property of Bay Medical Center and may be confidential. If you are not the intended addressee, you should not distribute, copy or disclose this e-mail. Please notify the sender immediately if you received this e-mail by mistake and delete this email from your system and destroy all printed copies. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet