Loralee: Please enlighten me! As far as I know FDA requirements extend to fixation time and NBF is required by some kit FDA approved, but not to how the tissues are processed. Even there are studies that show that xylene has extracting effects over some epitopes. I know that you cannot argue with FDA, but you can always validate any changes in your procedure. René J.
--- On Tue, 9/27/11, McMahon, Loralee A <loralee_mcma...@urmc.rochester.edu> wrote: From: McMahon, Loralee A <loralee_mcma...@urmc.rochester.edu> Subject: RE: [Histonet] Xylene sensitivity To: "Rene J Buesa" <rjbu...@yahoo.com>, "Histonet Listserv (E-mail)" <histonet@lists.utsouthwestern.edu>, "ShelleyD'Attilio" <sdatt...@stormontvail.org> Date: Tuesday, September 27, 2011, 9:40 AM Rene, I understand the desire to remove Xylene from the lab completely. But what if you are running FDA approved kits that are only FDA approved if the tissue is processed in Xylene? Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa [rjbu...@yahoo.com] Sent: Tuesday, September 27, 2011 9:33 AM To: Histonet Listserv (E-mail); ShelleyD'Attilio Subject: Re: [Histonet] Xylene sensitivity Shelley: As you correctly think this employee is most likely sensitive to xylene. Having a filtration system is the wrong solution. Is like having a pain when you use your arm and immobilizing it to prevent the pain. Your long term and complete solution is to eliminate xylene from your lab and I mean from ALL tasks using it now. Under separate cover I am sending 2 publications explaining how to do that. René J. --- On Tue, 9/27/11, D'Attilio, Shelley <sdatt...@stormontvail.org> wrote: From: D'Attilio, Shelley <sdatt...@stormontvail.org> Subject: [Histonet] Xylene sensitivity To: "Histonet Listserv (E-mail)" <histonet@lists.utsouthwestern.edu> Date: Tuesday, September 27, 2011, 9:27 AM Hi all, I have a new employee who is developing a scratchy, painful throat and some difficulty breathing when exposed to xylene (for instance, when the cover is raised on the coverslipper). This is her first job in a lab of any sort. We are investigating all the usual culprits--air handling system, hoods, allergies or virus unrelated to histology, etc. Right now she is wearing a PAPR to work, which is obviously not a long-term solution. Ultimately, I think we will conclude that this employee has a sensitivity to xylene and possibly other chemicals in the histology lab, as other employees are not complaining about symptoms related to chemicals. Does anyone have any experience with activated charcoal air cleaners? I am looking at a portable unit that sits on casters and provides 4 air exchanges per hour. It's not cheap at $1000, but well worth it if it will provide relief for this employee and allow her to continue her employment. Our lab is approximately 800-1000 sq. ft in size with 8 foot ceilings. We have 1 standard bio-hood for processing cytology fluids and 2 wall-mounted "air suckers" above our processors. I am open to any suggestions. Thanks, Shelley D'Attilio MT(ASCP) Manager, Chemistry, Cytology and Histology Dept. of Pathology and Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ****************************************************************************************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ****************************************************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet