Make your own with moldy bread. Works great!

G Micek 
Sent from my iPhone

On Oct 1, 2011, at 12:07 PM, histonet-requ...@lists.utsouthwestern.edu wrote:

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> Today's Topics:
> 
>   1. Control Tissue (Terry&Star Bullard)
>   2. Position Available @ NIH (Bethesda, MD)  (Jason Blaine)
>   3. Histonet Digest, Vol 94, Issue 40 Nothing down the drain
>      (Steve McClain)
>   4. washing the placenta (An Eerdekens)
>   5. Microtome Alignment Tool (Marcia Fisher)
>   6. RE: AFB Controls (Farish, Craig)
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Fri, 30 Sep 2011 13:29:43 -0400
> From: "Terry&Star Bullard" <tnsb...@gmail.com>
> Subject: [Histonet] Control Tissue
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
>    <cadvtc3zm+cqj-6fk_luk_3nlewd_9wb6yoq5qevwvbzj-pu...@mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
> 
> Hi all. Does anyone have a resource for control blocks? We are in need
> of fungus controls and are trying to save the cost of buying slides.
> Thanks all.
> 
> Sheila Haas
> Laboratory Supervisor
> MicroPath Laboratories, Inc
> 
> 
> Sheila,
> National Society for Histotechnology has a Tissue Control Bank, If you
> contact the person below she can help:
> 
> Melinda A Hamilton HT (ASCP)
> NSH Tissue Control Bank Curator
> Melinda Sokol <m.soko...@gmail.com>
> 
> Hope that help!
> Star Bullard HT(ASCP)
> 
> 
> ------------------------------
> 
> Message: 2
> Date: Fri, 30 Sep 2011 17:33:27 +0000
> From: Jason Blaine <jbla...@astrixinc.com>
> Subject: [Histonet] Position Available @ NIH (Bethesda, MD) 
> To: "histonet@lists.utsouthwestern.edu"
>    <histonet@lists.utsouthwestern.edu>
> Message-ID:
>    
> <A115DD9FF6F4A14D8DCF77948DCE580C74C98E@MBX021-W3-CA-1.exch021.domain.local>
>    
> Content-Type: text/plain; charset="us-ascii"
> 
> Seeking highly skilled scientist or histologist who is well versed in IHC.  
> This position is in the National Institute of Mental Health (NIMH) and thus 
> requires prior neuroscience and non-human primate experience.  Please note 
> that we are NOT necessarily seeking ASCP histology certificated individuals 
> and that prior related experience will be preferred over certification.
> 
> The position will support research in the NIMH.  The position will provide 
> histology support service for the complete laboratory (has 5 sections within 
> it).  This person will be the go to person for histology and IHC within the 
> lab and its 5 sections.  This individual will be expected to function as a 
> resource to all of the lab's not solely as a technician but rather as a 
> histology/IHC expert to advise the teams with their histology/IHC needs.  
> This will be a collaborative research effort and will not offer personal 
> independent research.  Non-human primate model is the principal concern.  
> Experience with IHC, Fluoro-ruby and green, D2, BrDu, tyrosine hyroxylase, 
> perfusion, brain removal and prep, and much more is desired.
> 
> If this opportunity is not right for you I ask that you point me in the 
> direction of someone who may be appropriately qualified - maybe a colleague 
> of yours, or former boss, or collaborator.  The opportunity is a fulltime, 
> salaried, indefinite, contractor position with benefits.
> 
> Qualified candidates please submit a current CV/resume (in MS Word format) to 
> jbla...@astrixinc.com.
> 
> Thanks -
> Jason Blaine
> jbla...@astrixinc.com
> 
> 
> 
> ------------------------------
> 
> Message: 3
> Date: Fri, 30 Sep 2011 14:08:27 -0400
> From: "Steve McClain" <ste...@mcclainlab.com>
> Subject: [Histonet] Histonet Digest, Vol 94, Issue 40 Nothing down the
>    drain
> To: <histonet@lists.utsouthwestern.edu>
> Message-ID:
>    <EE723B268CDC7F4E85D42139B6F5416337B061@ml1.McClainLabs.local>
> Content-Type: text/plain;    charset="us-ascii"
> 
> Yes nothing down the drain (except maybe the lithium carbonate bluing).
> 
> Hematoxylin goes into medical waste service.
> 
> Used Eosin gets reused a 10-20 ml at a time into the first absolute on
> the processors as a marker of carryover (as the later alcohols turn red,
> we have a visual indicator of the need to change, also helps with
> embedding since collagen stains more than epithelium/epidermis).
> 
> Alcohols after the de-waxing xylenes and from the processor purges go to
> the medical waste service.
> 
> Other alcohols get recycled.
> 
> All the Xylenes get recycled.
> 
> Liquid Waste from the recycler goes to medical waste service.
> 
> Paraffins go into the trash.
> 
> 
> 
> 
> 
> Steve
> 
> Steve A. McClain, MD
> 
> McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000
> 
> 
> 
> 
> 
> ------------------------------
> 
> Message: 4
> Date: Fri, 30 Sep 2011 21:18:10 +0200
> From: An Eerdekens <an.eerdek...@uzleuven.be>
> Subject: [Histonet] washing the placenta
> To: "'histonet@lists.utsouthwestern.edu'"
>    <histonet@lists.utsouthwestern.edu>
> Message-ID:
>    <09f2ba9a1e06c64d9f090ff0fd0d73179763afd...@ex2007-mbx-2.uz.kuleuven.ac.be>
>    
> Content-Type: text/plain; charset="us-ascii"
> 
> Dear all,
> 
> Is there someone with experience in washing of placentae before sampling?
> 
> Regards
> 
> Dr An Eerdekens
> 
> 
> ------------------------------
> 
> Message: 5
> Date: Fri, 30 Sep 2011 19:33:59 +0000
> From: Marcia Fisher <mfis...@ecrmc.org>
> Subject: [Histonet] Microtome Alignment Tool
> To: "histonet@lists.utsouthwestern.edu"
>    <histonet@lists.utsouthwestern.edu>
> Message-ID:
>    <bac5284d8bdc9b40819e2c75ed34d065188...@exmbx01.ecrmc.ci.el-centro.ca.us>
>    
> Content-Type: text/plain; charset="us-ascii"
> 
> We just purchased one from American Master Tech and really like the results.  
> We now have added this task to our monthly maintenance sheets.
> 
> Marcia Fisher
> Histology Supervisor/Lab Safety Officer
> El Centro Regional Medical Center
> 
> 
> ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended 
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> 
> 
> ------------------------------
> 
> Message: 6
> Date: Sat, 1 Oct 2011 20:57:25 +1000
> From: "Farish, Craig" <cfar...@csu.edu.au>
> Subject: [Histonet] RE: AFB Controls
> To: "histonet@lists.utsouthwestern.edu"
>    <histonet@lists.utsouthwestern.edu>
> Message-ID:
>    <3232733c82c90d439bf48c9fef3b7d1c2f78512...@mail01.csumain.csu.edu.au>
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> 
> Hi Pamela, try contacting your local large animal vet or a veterinary 
> diagnostic lab. Any case of Ovine or Bovine Johnne's disease would be a great 
> ZN positive control. The bacteria (mycobacterium avium subsp 
> paratuberculosis) is acid fast and congregates in huge numbers in the 
> terminal ileum and ileo-caecal junction (and also the caudal mesenteric lymph 
> node in smaller numbers).
> About the only problem i've ever come across is that there are so many AFBs 
> it may mask poorly stained section unless you look for individual bacteria in 
> the margins.
> Hope this helps
> Craig
> 
> Craig Farish
> Senior TO
> Veterinary Diagnostic Laboratory
> Charles Sturt University
> Wagga Wagga
> NSW, Australia
> 
> 
> ------------------------------
> 
> Message: 12
> Date: Thu, 29 Sep 2011 18:23:07 +0000
> From: "Marcum, Pamela A" <pamar...@uams.edu>
> Subject: [Histonet] AFB Controls
> To: "histonet@lists.utsouthwestern.edu"
>        <histonet@lists.utsouthwestern.edu>
> Message-ID:
>        <41d3a1af6fef0643bdc89e0516a6ea3209697...@mail2node2.ad.uams.edu>
> Content-Type: text/plain; charset="us-ascii"
> 
> Does anyone know where we can get a good AFB control?  Ours have exhausted 
> and we can't find a case here with a positive reading.
> 
> Best Regards,
> 
> Pamela A Marcum
> UAMS
> Slot 502
> 4301 W Markham Street
> Little Rock AR 72205
> Office: 501-686-7554
> Fax: 501-686-7151
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> End of Histonet Digest, Vol 95, Issue 1
> ***************************************

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