We have had this problem before. Make sure that the glacial acetic acid has been added to the light green stock solution. Cured our problem!!!
Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center 130 Fisher Road Berlin, VT 05641 802-371-4923 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of rg...@bmnet.com Sent: Tuesday, October 04, 2011 10:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Green counterstain problems Good Morning Histonetters! I've been having a counterstain problem with my GMS and PAS-F. Both stains work as they should until the green counterstain is applied. I cannot get my tissue (control or patient) to uptake the counterstain. I have varied time, temperature, even companies. I have good controls that have been in use for years without problems, until now. Only after an extended -10+ minutes,- can I get a blush of color in them. I've even called the slide company to ask if there has been a change to the charge coating I'm open to suggestions. Thanks! Renee Grow, BA., HT (ASCP) rg...@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax ________________________________ This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Tennessee Laws. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete this message from your computer. Direct questions to the Blount Memorial Hospital Privacy Officer at 865-977-4675. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet