CLIA-88 requirements can be found here: http://wwwn.cdc.gov/clia/regs/subpart_j.aspx. Local and state requirements may vary.
Eric Gilchrist, Supervisor UNC Oral and Maxillofacial Pathology Laboratory -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Tuesday, October 04, 2011 11:08 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 95, Issue 4 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Slide/Block Retention (Richard Cartun) 2. Cpt Charge (Anita Buchiane) 3. RE: Atlas of laboratory mouse histology (Hinsinger Julie) 4. RE: Cpt Charge (Weems, Joyce) 5. RE: decal question (Sarah Dysart) 6. RE: Cpt Charge (Setlak, Lisa) 7. RE: decal question (Bernice Frederick) 8. RE: decal question (Houston, Ronald) 9. RE: decal question (Patsy Ruegg) 10. Re: Slide/Block Retention (Bob Richmond) 11. RE: Atlas of laboratory mouse histology (Andrea Marion) 12. RE: Re: Slide/Block Retention (Blazek, Linda) 13. HP controls (Marcia Funk) 14. Atlas of laboratory mouse histology (Amos Brooks) 15. RE: decal question (Jennifer MacDonald) 16. RE: decal question (Edwards, Richard E.) 17. Re: Von Kossa Stain for calcium (Sherwood, Margaret) 18. Green counterstain problems (rg...@bmnet.com) 19. RELIA Histology Careers Bulletin Special Edition for Managers and Supervisors 10-4-2011 (Pam Barker) 20. RE: Green counterstain problems (Bell, Lynne) ---------------------------------------------------------------------- Message: 1 Date: Mon, 03 Oct 2011 13:20:42 -0400 From: "Richard Cartun" <rcar...@harthosp.org> Subject: Re: [Histonet] Slide/Block Retention To: <histonet@lists.utsouthwestern.edu>, "Michael J Dessoye" <mjdess...@wvhcs.org> Message-ID: <4e89b6aa.7400.007...@harthosp.org> Content-Type: text/plain; charset=US-ASCII We currently keep slides and paraffin blocks (surgical pathology) for 17 years; I would keep more, but laboratory administration keeps tell me to discard because we don't have sufficient storage space. It's not unusual for me to receive a request for a block(s) from the 1990's for IHC, molecular, or genetic testing especially now that we have entered the arena for "Personalized Medicine". Also, I find myself re-testing specimens from the 1990's and 2000's with new generation antibodies, probes, and detection systems. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Dessoye, Michael J" <mjdess...@wvhcs.org> 10/3/2011 9:50 AM >>> Hello Histonet, What policy is everyone following for slide and block retention? We are not CAP, however we currently keep slides and blocks for 10 years. My director wants to decrease that period to 2 years, which is the Joint Commission standard. I would like to keep 10 years because we frequently are asked to send slides for consult and review that are several years old. It is also helpful when looking for hard-to-find control tissue. However these reasons are apparently not sufficient. I'm leaning towards keeping the 10 year policy, but I need additional justification. Or does anyone think the 2 year period is sufficient? Thanks, Mike Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdess...@wvhcs.org <mailto:mjdess...@wvhcs.org> | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 3 Oct 2011 17:24:16 +0000 From: Anita Buchiane <abuchi...@bmhvt.org> Subject: [Histonet] Cpt Charge To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <4034e71604330c4b8e10d1538dfb2455c...@bmhexch02.bmhvt.org> Content-Type: text/plain; charset="us-ascii" Can we still only bill 88342 per antibody /per part or has that been changed? For instance: If we do a Calponin and an Actin on 3 blocks from one part do we charge 88342 x 2 or x 6? I thought I remember reading that it was changed to allow for charging per slide but I can not find the reference. Thanks _______________________________________________________________ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. _______________________________________________________________ ------------------------------ Message: 3 Date: Mon, 3 Oct 2011 13:27:52 -0400 From: "Hinsinger Julie" <julie.hinsin...@umontreal.ca> Subject: [Histonet] RE: Atlas of laboratory mouse histology To: <histonet@lists.utsouthwestern.edu> Message-ID: <070fb8db1efa214292c54dcbeceb27cf02566...@mapiudem2.sim.umontreal.ca> Content-Type: text/plain; charset="iso-8859-1" Just to let you know about the first answers: - There is an online atlas that was brought to my attention : http://www.mouseatlas.org/data/mouse/libraries/SM202 <http://www.mouseatlas.org/data/mouse/libraries/SM202> - And most referred book was Atlas of Mouse Development by Matthew H. Kaufman with a revised edition, printed in 2002. (Good for mouse embryo stages) I might have another question: As for adult organs, is there a general histology atlas you refer to? In our lab, we use "Pathology of the Mouse" Robert R. Maronpot <http://www.amazon.ca/s?_encoding=UTF8&search-alias=books-ca&field-author=Robert%20R.%20Maronpot> As we work on both human and mouse tissues, I have found a new book that might be interesting "Comparative Anatomy and Histology: A Mouse and Human Atlas" Have you heard about it? It will be released in December 2011. http://www.amazon.ca/Comparative-Anatomy-Histology-Expert-Consult/dp/0123813611/ref=sr_1_1?s=books&ie=UTF8&qid=1317661829&sr=1-1 It aims at the new mouse investigator as well as medical and veterinary pathologists who need to expand their knowledge base into comparative anatomy and histology. It guides the reader through normal mouse anatomy and histology using direct comparison to the human. The side by side comparison of mouse and human tissues highlight the unique biology of the mouse, which has great impact on the validation of mouse models of human disease. Seems great! I don't know... Julie De : Hinsinger Julie Envoy? : 3 octobre 2011 11:41 ? : 'histonet@lists.utsouthwestern.edu' Objet : Atlas of laboratory mouse histology Does anyone know of an atlas of mouse histology? Thanks for your comments Julie Hinsinger Histology Facility Manager IRIC - Pav. M. Coutu - Local 3440 2950, chemin polytechnique - Montr?al (Qc) H3T 1J4 ------------------------------ Message: 4 Date: Mon, 3 Oct 2011 13:54:23 -0400 From: "Weems, Joyce" <jwe...@sjha.org> Subject: [Histonet] RE: Cpt Charge To: Anita Buchiane <abuchi...@bmhvt.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <92ad9b20a6c38c4587a9febe3a30e1640827b74...@chexcms10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" You can now charge per block. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anita Buchiane Sent: Monday, October 03, 2011 13:24 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cpt Charge Can we still only bill 88342 per antibody /per part or has that been changed? For instance: If we do a Calponin and an Actin on 3 blocks from one part do we charge 88342 x 2 or x 6? I thought I remember reading that it was changed to allow for charging per slide but I can not find the reference. Thanks _______________________________________________________________ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. _______________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 5 Date: Mon, 3 Oct 2011 17:55:04 +0000 From: Sarah Dysart <sdys...@mirnarx.com> Subject: RE: [Histonet] decal question To: Patsy Ruegg <pru...@ihctech.net>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <8a70a9b2ecdd084dacfe6c59fcf86d507...@sn2prd0702mb110.namprd07.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" Formalin? Isn't all decal acid decal? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, October 03, 2011 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decal question Hi Everyone, I have a new student taking course work at UND and using my lab for her practical clinical site. She took a test on decal today and there was a question we didn't know the answer to. What fixative should not be used for acid decalcification? Would it be osmium tetroxide? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email <mailto:pru...@ihctech.net> pru...@ihctech.net web site <http://www.ihctech.net> www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 3 Oct 2011 13:01:04 -0500 From: "Setlak, Lisa" <lset...@childrensmemorial.org> Subject: [Histonet] RE: Cpt Charge To: "'Weems, Joyce'" <jwe...@sjha.org>, Anita Buchiane <abuchi...@bmhvt.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <7111db39d045004c9cf29e79c71b28bc1093d4d...@cmhexcc01mbx.childrensmemorial.org> Content-Type: text/plain; charset="us-ascii" Where we can find a reference to this.... I know I'll get questions when I change it? Thanks, Lisa Children's Memorial Hospital Chicago, Il -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, October 03, 2011 12:54 PM To: Anita Buchiane; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cpt Charge You can now charge per block. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anita Buchiane Sent: Monday, October 03, 2011 13:24 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cpt Charge Can we still only bill 88342 per antibody /per part or has that been changed? For instance: If we do a Calponin and an Actin on 3 blocks from one part do we charge 88342 x 2 or x 6? I thought I remember reading that it was changed to allow for charging per slide but I can not find the reference. Thanks _______________________________________________________________ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. _______________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 3 Oct 2011 18:17:59 +0000 From: Bernice Frederick <b-freder...@northwestern.edu> Subject: RE: [Histonet] decal question To: Patsy Ruegg <pru...@ihctech.net>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <62c639732d3f274daced033ebdf6adaf1e17a...@evcspmbx3.ads.northwestern.edu> Content-Type: text/plain; charset="us-ascii" Ann Preece states acid decal uses aqueous solutions od either formic,nitric or trichloroacetic acid. Other methods mentioned are Ion-exchange resin,electrical ionization and chelation. The histo bible! Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, October 03, 2011 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decal question Hi Everyone, I have a new student taking course work at UND and using my lab for her practical clinical site. She took a test on decal today and there was a question we didn't know the answer to. What fixative should not be used for acid decalcification? Would it be osmium tetroxide? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email <mailto:pru...@ihctech.net> pru...@ihctech.net web site <http://www.ihctech.net> www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Mon, 3 Oct 2011 18:25:32 +0000 From: "Houston, Ronald" <ronald.hous...@nationwidechildrens.org> Subject: RE: [Histonet] decal question To: 'Patsy Ruegg' <pru...@ihctech.net>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <e5d0cd2352e46545a0c0ebe308ccce5317b...@l1perdwxmb01.childrensroot.net> Content-Type: text/plain; charset="us-ascii" Alcoholic formalin or 70% ethanol as, quoting Gayle Callis, "alcohol can slow or prevent decalcification" Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, October 03, 2011 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decal question Hi Everyone, I have a new student taking course work at UND and using my lab for her practical clinical site. She took a test on decal today and there was a question we didn't know the answer to. What fixative should not be used for acid decalcification? Would it be osmium tetroxide? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email <mailto:pru...@ihctech.net> pru...@ihctech.net web site <http://www.ihctech.net> www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ------------------------------ Message: 9 Date: Mon, 3 Oct 2011 12:25:52 -0600 From: "Patsy Ruegg" <pru...@ihctech.net> Subject: RE: [Histonet] decal question To: "'Sarah Dysart'" <sdys...@mirnarx.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <7104CA2AA7E64D859B3B1084AD323E5B@Patsyoffice> Content-Type: text/plain; charset="us-ascii" I think I miss phrased this question, we thought they were asking what fixative should never be used if you are planning to do acid decal after fixation? We still think it must be osmium???? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pru...@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: Sarah Dysart [mailto:sdys...@mirnarx.com] Sent: Monday, October 03, 2011 11:55 AM To: Patsy Ruegg; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] decal question Formalin? Isn't all decal acid decal? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, October 03, 2011 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decal question Hi Everyone, I have a new student taking course work at UND and using my lab for her practical clinical site. She took a test on decal today and there was a question we didn't know the answer to. What fixative should not be used for acid decalcification? Would it be osmium tetroxide? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email <mailto:pru...@ihctech.net> pru...@ihctech.net web site <http://www.ihctech.net> www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Mon, 3 Oct 2011 14:30:26 -0400 From: Bob Richmond <rsrichm...@gmail.com> Subject: [Histonet] Re: Slide/Block Retention To: histonet@lists.utsouthwestern.edu Message-ID: <CAOKsRH43itNP+YL_fgqq_0VGaoLbCU1=f18y4-qms2vtkup...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 I don't care what the herrn inshpektors say, the fact is that patient care - with the rapid growth of genetic and other molecular techniques - I think 10 years is the minimum for retaining paraffin blocks, and I'd be a lot more comfortable with 20. In the future I expect we'll be retrieving blocks to do studies on members of patients' families. And if your kid flunks out of college, I reckon you can sue the OB that delivered him - so that's >20 years for placentas. An issue rarely mentioned: when a tissue lab closes, everything is often discarded. We need requirements for tissue retention when labs close. I have no idea how this could be done. But as regulations increase and our economy declines, there'll be a lot of tissue labs closing in the next decade. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 11 Date: Mon, 3 Oct 2011 14:34:07 -0500 From: "Andrea Marion" <amar...@uic.edu> Subject: [Histonet] RE: Atlas of laboratory mouse histology To: histonet@lists.utsouthwestern.edu Cc: julie.hinsin...@umontreal.ca Message-ID: <0c00e9909cf1a85757b0f687b639347f.squir...@webmail.uic.edu> Content-Type: text/plain;charset=iso-8859-1 Hi Julie - Other than the ones you already found, I have two (okay 3) more recommendations: 1- Theiler's classic The House Mouse: Atlas of Embryonic Development has a great histology of mouse embryos. I use it side-by-side with the Kaufmann text for embryo histology. It's freely available for download, which is very handy: http://www.emouseatlas.org/Atlas/Theiler_book_download.html 2- There is a quick online guide for adult mouse histology at http://ctrgenpath.net/static/atlas/mousehistology/Windows/introduction.html It's a nice page for quickly checking something, but there is not a lot of in depth coverage. 3 - I also suggest searching on the mouse research listserv, I've seen it discussed there before and a quick search turned up a number of recommendations: http://www.informatics.jax.org/lyris-cgi/lyris.pl?visit=mgi-list Good luck, Andrea Andrea Marion Graduate Student University of Illinois at Chicago [Histonet] RE: Atlas of laboratory mouse histology Hinsinger Julie julie.hinsinger <@t> umontreal.ca Mon Oct 3 12:27:52 CDT 2011 Previous message: [Histonet] RE: Cpt Charge Next message: [Histonet] decal question Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] Just to let you know about the first answers: - There is an online atlas that was brought to my attention : http://www.mouseatlas.org/data/mouse/libraries/SM202 <http://www.mouseatlas.org/data/mouse/libraries/SM202> - And most referred book was Atlas of Mouse Development by Matthew H. Kaufman with a revised edition, printed in 2002. (Good for mouse embryo stages) I might have another question: As for adult organs, is there a general histology atlas you refer to? In our lab, we use "Pathology of the Mouse" Robert R. Maronpot <http://www.amazon.ca/s?_encoding=UTF8&search-alias=books-ca&field-author=Robert%20R.%20Maronpot> As we work on both human and mouse tissues, I have found a new book that might be interesting "Comparative Anatomy and Histology: A Mouse and Human Atlas" Have you heard about it? It will be released in December 2011. http://www.amazon.ca/Comparative-Anatomy-Histology-Expert-Consult/dp/0123813611/ref=sr_1_1?s=books&ie=UTF8&qid=1317661829&sr=1-1 It aims at the new mouse investigator as well as medical and veterinary pathologists who need to expand their knowledge base into comparative anatomy and histology. It guides the reader through normal mouse anatomy and histology using direct comparison to the human. The side by side comparison of mouse and human tissues highlight the unique biology of the mouse, which has great impact on the validation of mouse models of human disease. Seems great! I don't know... Julie De : Hinsinger Julie Envoy? : 3 octobre 2011 11:41 ? : 'histonet <@t> lists.utsouthwestern.edu' Objet : Atlas of laboratory mouse histology Does anyone know of an atlas of mouse histology? Thanks for your comments Julie Hinsinger Histology Facility Manager IRIC - Pav. M. Coutu - Local 3440 2950, chemin polytechnique - Montr?al (Qc) H3T 1J4 ------------------------------ Message: 12 Date: Mon, 3 Oct 2011 15:50:15 -0400 From: "Blazek, Linda" <lbla...@digestivespecialists.com> Subject: RE: [Histonet] Re: Slide/Block Retention To: 'Bob Richmond' <rsrichm...@gmail.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <5a2bd13465e061429d6455c8d6b40e39127401b...@ibmb7exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" There is an inspection question that addresses what happens to material if a lab closes. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Monday, October 03, 2011 2:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Slide/Block Retention I don't care what the herrn inshpektors say, the fact is that patient care - with the rapid growth of genetic and other molecular techniques - I think 10 years is the minimum for retaining paraffin blocks, and I'd be a lot more comfortable with 20. In the future I expect we'll be retrieving blocks to do studies on members of patients' families. And if your kid flunks out of college, I reckon you can sue the OB that delivered him - so that's >20 years for placentas. An issue rarely mentioned: when a tissue lab closes, everything is often discarded. We need requirements for tissue retention when labs close. I have no idea how this could be done. But as regulations increase and our economy declines, there'll be a lot of tissue labs closing in the next decade. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Mon, 03 Oct 2011 16:20:34 -0400 From: "Marcia Funk" <fu...@mercyhealth.com> Subject: [Histonet] HP controls To: <Histonet@lists.utsouthwestern.edu> Message-ID: <4e89d2c2.e948.00a...@mercyhealth.com> Content-Type: text/plain; charset=US-ASCII We are having difficult with the HP control for IHC staining - If you are purchasing controls or if you have controls that you might share I would really appreciate your help. Thanks so much -Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 ------------------------------ Message: 14 Date: Mon, 3 Oct 2011 17:27:28 -0400 From: Amos Brooks <amosbro...@gmail.com> Subject: [Histonet] Atlas of laboratory mouse histology To: histonet@lists.utsouthwestern.edu Message-ID: <CAC95ki8fiTp-N9h_wai7p7HCT+2OzEq950ePwPdAJb-KHuTO=a...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi, I would be interested in this reference as well. Please post to the group if you have any suggestions. Amos On Mon, Oct 3, 2011 at 1:00 PM, <histonet-requ...@lists.utsouthwestern.edu>wrote: > Message: 10 > Date: Mon, 3 Oct 2011 11:40:55 -0400 > From: "Hinsinger Julie" <julie.hinsin...@umontreal.ca> > Subject: [Histonet] Atlas of laboratory mouse histology > To: <histonet@lists.utsouthwestern.edu> > Message-ID: > < > 070fb8db1efa214292c54dcbeceb27cf02566...@mapiudem2.sim.umontreal.ca> > Content-Type: text/plain; charset="iso-8859-1" > > Does anyone know of an atlas of mouse histology? > > Thanks for your comments > > > > Julie Hinsinger > > Histology Facility Manager > ------------------------------ Message: 15 Date: Tue, 4 Oct 2011 04:46:50 -0700 From: Jennifer MacDonald <jmacdon...@mtsac.edu> Subject: RE: [Histonet] decal question To: "Patsy Ruegg" <pru...@ihctech.net> Cc: histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu Message-ID: <of0f04e57d.1026c4c4-on8825791f.00409d53-8825791f.0040b...@mtsac.edu> Content-Type: text/plain; charset="US-ASCII" Was there more to the question? You would not want to use any fixatives containing metals, such as mercury, if you will be doing end-point decalcification determination using x-rays. "Patsy Ruegg" <pru...@ihctech.net> Sent by: histonet-boun...@lists.utsouthwestern.edu 10/03/2011 11:27 AM To "'Sarah Dysart'" <sdys...@mirnarx.com>, <histonet@lists.utsouthwestern.edu> cc Subject RE: [Histonet] decal question I think I miss phrased this question, we thought they were asking what fixative should never be used if you are planning to do acid decal after fixation? We still think it must be osmium???? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pru...@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: Sarah Dysart [mailto:sdys...@mirnarx.com] Sent: Monday, October 03, 2011 11:55 AM To: Patsy Ruegg; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] decal question Formalin? Isn't all decal acid decal? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, October 03, 2011 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decal question Hi Everyone, I have a new student taking course work at UND and using my lab for her practical clinical site. She took a test on decal today and there was a question we didn't know the answer to. What fixative should not be used for acid decalcification? Would it be osmium tetroxide? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email <mailto:pru...@ihctech.net> pru...@ihctech.net web site <http://www.ihctech.net> www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Tue, 4 Oct 2011 13:17:15 +0100 From: "Edwards, Richard E." <r...@leicester.ac.uk> Subject: RE: [Histonet] decal question To: 'Jennifer MacDonald' <jmacdon...@mtsac.edu>, Patsy Ruegg <pru...@ihctech.net> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, "histonet-boun...@lists.utsouthwestern.edu" <histonet-boun...@lists.utsouthwestern.edu> Message-ID: <7722595275a4dd4fa225b92cdbf174a101a4edf88...@exc-mbx3.cfs.le.ac.uk> Content-Type: text/plain; charset="us-ascii" Was it decalcifying a tissue fixed in a formalin based fixative using a hydrochloric acid containing decalcifying fluid? as even storage of HCl and formalin in the same area is a bad plan. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: 04 October 2011 12:47 To: Patsy Ruegg Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] decal question Was there more to the question? You would not want to use any fixatives containing metals, such as mercury, if you will be doing end-point decalcification determination using x-rays. "Patsy Ruegg" <pru...@ihctech.net> Sent by: histonet-boun...@lists.utsouthwestern.edu 10/03/2011 11:27 AM To "'Sarah Dysart'" <sdys...@mirnarx.com>, <histonet@lists.utsouthwestern.edu> cc Subject RE: [Histonet] decal question I think I miss phrased this question, we thought they were asking what fixative should never be used if you are planning to do acid decal after fixation? We still think it must be osmium???? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pru...@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: Sarah Dysart [mailto:sdys...@mirnarx.com] Sent: Monday, October 03, 2011 11:55 AM To: Patsy Ruegg; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] decal question Formalin? Isn't all decal acid decal? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, October 03, 2011 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decal question Hi Everyone, I have a new student taking course work at UND and using my lab for her practical clinical site. She took a test on decal today and there was a question we didn't know the answer to. What fixative should not be used for acid decalcification? Would it be osmium tetroxide? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email <mailto:pru...@ihctech.net> pru...@ihctech.net web site <http://www.ihctech.net> www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Tue, 4 Oct 2011 10:36:30 -0400 From: "Sherwood, Margaret" <msherw...@partners.org> Subject: Re: [Histonet] Von Kossa Stain for calcium To: <histonet@lists.utsouthwestern.edu> Message-ID: <073ae2bea1c2ba4a8837ab6c4b943d9708db5...@phsxmb30.partners.org> Content-Type: text/plain; charset="us-ascii" We are a research lab and don't do a big volume of special stains. We just received a project for 35 slides that need Von Kossa Stain. I acid-washed some small glass staining dishes-no rack required (in the past, we only had a few requests). Can I run slides in plastic slide holders (24-place) in an acid-washed beaker for the silver nitrate step? Any tips would be appreciated. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 18 Date: Tue, 4 Oct 2011 10:48:25 -0400 From: rg...@bmnet.com Subject: [Histonet] Green counterstain problems To: histonet@lists.utsouthwestern.edu Message-ID: <of30a89d15.b402dd42-on8525791f.0050c208-8525791f.00515...@bmnet.com> Content-Type: text/plain; charset=US-ASCII Good Morning Histonetters! I've been having a counterstain problem with my GMS and PAS-F. Both stains work as they should until the green counterstain is applied. I cannot get my tissue (control or patient) to uptake the counterstain. I have varied time, temperature, even companies. I have good controls that have been in use for years without problems, until now. Only after an extended -10+ minutes,- can I get a blush of color in them. I've even called the slide company to ask if there has been a change to the charge coating I'm open to suggestions. Thanks! Renee Grow, BA., HT (ASCP) rg...@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax ________________________________ This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Tennessee Laws. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete this message from your computer. Direct questions to the Blount Memorial Hospital Privacy Officer at 865-977-4675. ------------------------------ Message: 19 Date: Tue, 4 Oct 2011 10:51:50 -0400 From: "Pam Barker" <rel...@earthlink.net> Subject: [Histonet] RELIA Histology Careers Bulletin Special Edition for Managers and Supervisors 10-4-2011 To: "'Histonet'" <histonet@lists.utsouthwestern.edu> Message-ID: <FF6FFEF1FF3E4934AA326C18A4722E1E@ownerf1abaad51> Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters!! What would make the perfect management position? Is it the location? Perhaps the type of lab? How about the size of the staff/lab? Maybe it?s the Hours? The Money and/or benefits? Could it be that a particular situation is the next logical step in your career? More than likely the answer is all of the above in different degrees. That is why I am contacting you with this special bulletin. I am starting to get a lot of management opportunities and I wanted to touch base and let you know. Your next opportunity might be just around the corner and I might have it for you. If you are looking for a position right now please contact me right away. We can talk about my current positions OR about a customized search on your behalf. If you aren?t looking right away but want to let me know what would make a perfect job for you so that I could keep an eye out, that would be great too. To do that just shoot me an email at rel...@earthlink.net or call me toll free at 866-607-3542. Here is a list of my current managerial opportunities: Pathology Lab Manager ? Modesto, CA Assistant Histology Supervisor ? Austin, TX Histology Supervisor ? Portland, OR Histology Manager ? Long Island, NY All of these clients offer autonomy in your position and histology staff eager to welcome their new manager. I really appreciate you taking time out of your busy day to read my e-mail Thanks Again! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ------------------------------ Message: 20 Date: Tue, 4 Oct 2011 10:56:43 -0400 From: "Bell, Lynne" <lynne.b...@cvmc.org> Subject: RE: [Histonet] Green counterstain problems To: "'rg...@bmnet.com'" <rg...@bmnet.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <f9e5562685af9b498d9d8c1ec996d2440f73465...@cvmc-email.cvmc.org> Content-Type: text/plain; charset="us-ascii" We have had this problem before. Make sure that the glacial acetic acid has been added to the light green stock solution. Cured our problem!!! Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center 130 Fisher Road Berlin, VT 05641 802-371-4923 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of rg...@bmnet.com Sent: Tuesday, October 04, 2011 10:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Green counterstain problems Good Morning Histonetters! I've been having a counterstain problem with my GMS and PAS-F. Both stains work as they should until the green counterstain is applied. I cannot get my tissue (control or patient) to uptake the counterstain. I have varied time, temperature, even companies. I have good controls that have been in use for years without problems, until now. Only after an extended -10+ minutes,- can I get a blush of color in them. I've even called the slide company to ask if there has been a change to the charge coating I'm open to suggestions. Thanks! Renee Grow, BA., HT (ASCP) rg...@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax ________________________________ This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Tennessee Laws. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete this message from your computer. Direct questions to the Blount Memorial Hospital Privacy Officer at 865-977-4675. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 95, Issue 4 *************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet