Thanks. Thank God that the company that supplied the product contacted her and set her straight by telling her the same information you guys have told me. I even pointed the chapter from the Frieda Carson's book again, when it clearly says they are the same thing. Now she understood! Thank God I was getting frustrated because she did not agreed with me :(.
On Thu, Oct 6, 2011 at 9:12 AM, Rene J Buesa <rjbu...@yahoo.com> wrote: > Jenny: > Formaldehyde is the GAS methanal that dissolves in water at a maximum > concentration between 37-40% vol./vol. To prevent its polymerization, small > amounts of mathanol (an alcohol)are added. > The 10% solution formaldehyde contains between 3.7 and 4.0% of formaldehyde > and is called "formalin" in many countries and "formol" in Europe. > So formalin is a 3.7 to 4.0% solution of formaldehyde. > So a 10% formalin = 3.7-4.0% formaldehyde. > NBF is prepared by added phosphate salts to prevent the formalin to acidify > and is used to fix tissues. > It is a pitty that your supervisor has so little knowledge about the area > she is supposed to supervise, but I am not do not know why, but I am > not. surprised. > To your question: SHE is wrong, really wrong! > René J. > > > --- On *Wed, 10/5/11, Jenny Vega <histotech...@gmail.com>* wrote: > > > From: Jenny Vega <histotech...@gmail.com> > Subject: [Histonet] Difference between Neutral Buffered Formalin 10% and NB > formaldehyde 3.7 to 4% > To: histonet@lists.utsouthwestern.edu > Date: Wednesday, October 5, 2011, 7:03 PM > > Ok I want to know the diferrence between Neutral Buffered Formalin 10% and > NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same > thing and in the book from Frieda Carson it says that but my supervisor > swears they are different chemicals. In the laboratory she has used 10% > neutral buffered formalin all the time, but when we ran out of it we were > sent NB formaldehyde 3.7 to 4 %she sent it back and we were sent NB > formaldehyde 3.7 to 4% once again. She says that 10% NBF is more > concentrated and pure than 3.7 to 4 % formaldehyde and that the tissues are > going to putrefied if they are put in that solution. > > Am I wrong or is she wrong? thanks > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu<http://us.mc657.mail.yahoo.com/mc/compose?to=Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
