Carmen, I routinely stain 40-60 micron sections for IHC. I currently run all my IHC with a free-floating protocol which allows for maximum staining penetration from both sides of the section and then mount them on the slides. In the past, we mounted the 60um sections on (300 bloom) gelatin coated slides that were processed by hand in a humidity chamber. If you would like additional information about this technique, please send me an email.
Regards, Tina Montina J. Van Meter, HT (ASCP) Lab Manager Dept. of Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Thursday, October 13, 2011 9:30 AM To: 'Carmen Maria Garcia Pascual'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thick cryosections I don't care what kink of slides you use if you cut 50 micron thick sections and try to do IHC you are going to lose some or all sections, in my experience. The only thing that might work and even this is questionable because of the thickness is the tape transfer system but I really doubt you are going to be able to get sections that thick to stick to the polymer coated slide and not come off with the tape. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carmen Maria Garcia Pascual Sent: Tuesday, October 11, 2011 9:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thick cryosections Good afternoon everyone! I have made 50um mice ovarian cryosections and I have used superfrost plus slides. But some of them fall of the slides when I perfomed the immuno, one colleague has told me that this is because the cuts are too thick, and I think she is rigth. I don't know if there is some thing that I can make to avoid this, like using another kind of slides... Thanks!!!!!!!!!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet