If the antibody has precipitated, it may be impossible to restore activity. The glycerol is present to keep the suspension in liquid form - and it sounds like you no longer have a liquid. Try adding an appropriate suspension buffer and keep your fingers crossed. If you do not have previous experience with this antibody and know what to expect regarding performance, I would discard it and re-order.
Tresa -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sarah Tabatabaei Sent: Friday, October 28, 2011 9:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How to save dried antibody? Dear All, We have purchased a sheep anti-CGRP antibody for our fluorescent IHC study on human tissue and kept it in -20 for a while. It came in liquid but now it's all stiff and looks like it's being crystallized. I cannot pipet any of it. It has hardened and looks like a piece of glass at bottom of its container. I tried to re-suspend it with the same amount of glycerol, but the antibody doesn't seem to be mixing with it. Does anyone know how to save this antibody? How can I bring it back to its liquid state? Thank you for your time Sarah Sadat, DDS MSc Student, Dental Sciences McGill University Montreal QC. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet